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Cytotoxic Activities of Total Saponins from Plena Clematis on Human Tumor Cell Lines In Vitro
- Chinese Journal of Integrative Medicine Vol. 24, Issue 10, Pages: 763-767(2018)
- Affiliations：
1. Department of Pharmacy, Fujian Health College,Fuzhou,China
2. Department of Thoracic Surgery, Fujian Provincial Hospital, Provincial Clinical College of Fujian Medical University,Fuzhou,China
- Author bio：
- Funds：Supported by the Medical Elite Cultivation Program of Fujian Province, China (No. 2016-ZQN-75) and the Medical Innovation Subject of Fujian Province, China (No. 2012-CX-5)
DOI：10.1007/s11655-018-2839-z
CLC：Published：2018，
Published Online：21 June 2018，
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Zhu, Fr., Li, Yn., He, Sl. et al. Cytotoxic Activities of Total Saponins from Plena Clematis on Human Tumor Cell Lines In Vitro., Chin. J. Integr. Med. 24, 763–767 (2018). https://doi.org/10.1007/s11655-018-2839-z
Fu-rong Zhu, Yong-ning Li, Shu-lan He, et al. Cytotoxic Activities of Total Saponins from Plena Clematis on Human Tumor Cell Lines In Vitro. [J]. Chinese Journal of Integrative Medicine 24(10):763-767(2018)
Zhu, Fr., Li, Yn., He, Sl. et al. Cytotoxic Activities of Total Saponins from Plena Clematis on Human Tumor Cell Lines In Vitro., Chin. J. Integr. Med. 24, 763–767 (2018). https://doi.org/10.1007/s11655-018-2839-z DOI：
Fu-rong Zhu, Yong-ning Li, Shu-lan He, et al. Cytotoxic Activities of Total Saponins from Plena Clematis on Human Tumor Cell Lines In Vitro. [J]. Chinese Journal of Integrative Medicine 24(10):763-767(2018) DOI： 10.1007/s11655-018-2839-z.
摘要
To investigate the anti-proliferative effects of saponins prepared from Plena Clematis (PC) cultured in Fujian Province
China on 4 human tumor cell lines and its possible anti-tumor mechanism. The growth inhibition assays of saponins on human esophageal squamous carcinoma cell line (EC9706)
human hepatoma cell line (HepG-2)
human oral cancer cell line (KB) and human gastric cancer cell line (BGC-823) were evaluated in vitro by thiazolyl blue (MTT) method. The inhibitory effects on EC9706 treated with different concentrations of saponins (15.62
31.25
62.50
125
250 and 500 μg/mL) were performed in vitro by MTT method. The morphology and nuclear staining with acridine orange/ethidium bromide of EC9706 cells treated with saponins were illustrated under an inverted phase fluorescence microscope. The apoptotic effects of saponins were further evaluated by annexin-V/propidium iodide dual staining experiment to examine the occurrence of phosphatidylserine externalization onto the cell surface by a flflow cytometer. MTT assay showed that the saponins could inhibit the proliferation of 4 tumor cell lines. Among them
the maximum inhibition rate of 73.1% was detected in EC9706 cells at the saponins concentration of 250 μg/mL for 24 h. Further investigation indicated that the saponins induced EC9706 cells apoposis. The EC9706 cells presented apoptotic characteristics when treated with saponins
including that the morphologies of EC9706 cells were appeared round-shaped with higher refraction
and the cell nuclear stained orange with EB after 250 μg/mL saponins exposure. The flow cytometry analysis results showed that the induction of cell cycle arrest in apoptotic system may participate in the anti-proliferative activity of saponins on EC9706 cells. The saponins from PC exhibited significant cytotoxicity against human EC9706
KB
BGC-823
and HepG-2 cells and might be beneficial to development of ethnic pharmaceutical plant for potential anti-tumor drugs.
Abstract
To investigate the anti-proliferative effects of saponins prepared from Plena Clematis (PC) cultured in Fujian Province
China on 4 human tumor cell lines and its possible anti-tumor mechanism. The growth inhibition assays of saponins on human esophageal squamous carcinoma cell line (EC9706)
human hepatoma cell line (HepG-2)
human oral cancer cell line (KB) and human gastric cancer cell line (BGC-823) were evaluated in vitro by thiazolyl blue (MTT) method. The inhibitory effects on EC9706 treated with different concentrations of saponins (15.62
31.25
62.50
125
250 and 500 μg/mL) were performed in vitro by MTT method. The morphology and nuclear staining with acridine orange/ethidium bromide of EC9706 cells treated with saponins were illustrated under an inverted phase fluorescence microscope. The apoptotic effects of saponins were further evaluated by annexin-V/propidium iodide dual staining experiment to examine the occurrence of phosphatidylserine externalization onto the cell surface by a flflow cytometer. MTT assay showed that the saponins could inhibit the proliferation of 4 tumor cell lines. Among them
the maximum inhibition rate of 73.1% was detected in EC9706 cells at the saponins concentration of 250 μg/mL for 24 h. Further investigation indicated that the saponins induced EC9706 cells apoposis. The EC9706 cells presented apoptotic characteristics when treated with saponins
including that the morphologies of EC9706 cells were appeared round-shaped with higher refraction
and the cell nuclear stained orange with EB after 250 μg/mL saponins exposure. The flow cytometry analysis results showed that the induction of cell cycle arrest in apoptotic system may participate in the anti-proliferative activity of saponins on EC9706 cells. The saponins from PC exhibited significant cytotoxicity against human EC9706
KB
BGC-823
and HepG-2 cells and might be beneficial to development of ethnic pharmaceutical plant for potential anti-tumor drugs.
关键词
Plena ClematisClematis florida var. plena Dcytotoxic activitysaponinshuman esophageal cancer cell
Keywords
Plena ClematisClematis florida var. plena Dcytotoxic activitysaponinshuman esophageal cancer cell
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