Pien Tze Huang (片仔癀) Overcomes Doxorubicin Resistance and Inhibits Epithelial-Mesenchymal Transition in MCF-7/ADR Cells

Xi CHEN; Fei QI; A-ling SHEN; Jian-feng CHU; Thomas Joseph Sferra; You-qin CHEN; Jun PENG

doi:10.1007/s11655-018-2992-4

Your Location：

Home >

Browse articles >

Pien Tze Huang (片仔癀) Overcomes Doxorubicin Resistance and Inhibits Epithelial-Mesenchymal Transition in MCF-7/ADR Cells

Original Article | Updated：2021-08-27

- Pien Tze Huang (片仔癀) Overcomes Doxorubicin Resistance and Inhibits Epithelial-Mesenchymal Transition in MCF-7/ADR Cells
- Chinese Journal of Integrative Medicine Vol. 25, Issue 8, Pages: 598-603(2019)
- Affiliations：
  
  1.Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou (350122), China
  2.Department of Oncology, Fuzhou General Hospital of Nanjing Military Command, Fuzhou (350003), China
  3.Fujian Key Laboratory of Integrative Medicine on Geriatric, Fujian University of Traditional Chinese Medicine, Fuzhou (350122), China
  4.School of Medicine, Case Western Reserve University, Cleveland, OH (44106), USA
- Author bio：
  
  Correspondence to: Dr. PENG Jun, Tel: 86-591-22861303, E-mail: pjunlab@hotmail.com
- Funds：
- DOI：10.1007/s11655-018-2992-4
  CLC：
- Published：01 August 2019，
  
  Published Online：22 August 2018，
  
  Accepted：14 July 2017
Scan for full text
Xi CHEN, Fei QI, A-ling SHEN, et al. Pien Tze Huang (片仔癀) Overcomes Doxorubicin Resistance and Inhibits Epithelial-Mesenchymal Transition in MCF-7/ADR Cells. [J]. Chinese Journal of Integrative Medicine 25(8):598-603(2019)
DOI：

Xi CHEN, Fei QI, A-ling SHEN, et al. Pien Tze Huang (片仔癀) Overcomes Doxorubicin Resistance and Inhibits Epithelial-Mesenchymal Transition in MCF-7/ADR Cells. [J]. Chinese Journal of Integrative Medicine 25(8):598-603(2019) DOI： 10.1007/s11655-018-2992-4.

摘要

Abstract

Objective:

To evaluate the effect of Pien Tze Huang (片仔癀

PZH) on breast cancer chemoresistance and related epithelial-mesenchymal transition (EMT) and investigate the underlying mechanisms.

Methods:

3-(4

5-Dimethyl-2-thiazolyl)-2

5-diphenyl-2-H-tetrazolium bromide (MTT) assay was used to determine the cell viability. Adriamycin (ADR) staining observed by fluorescence microscope was performed to detect the accumulation of ADR. Transwell assay was used to analyze the cell migration and invasion. Western-blot was performed to detect the protein expression of related genes.

Results:

MCF-7/ADR cells were resistant to ADR treatment

and PZH treatment inhibited the viability of MCF-7/ADR cells in a dose-dependent manner. PZH treatment also increased the intercellular accumulation of ADR and down-regulated the expression of ABCG2 and ABCB1 in MCF-7/ADR cells (

0.05). In addition

PZH treatment inhibited EMT

migration and invasion of MCF-7/ADR cells (

0.05). Moreover

PZH suppressed activation of transforming growth factor β1 (TGF-β) signaling in MCF-7/ADR cells (

0.05).

Conclusion:

PZH treatment can effectively overcome chemoresistance via down-regulating ABCG2

ABCB1 and inhibit EMT in ADR resistant human breast cancer cells via suppression of the TGF-β1 pathway.

关键词

Keywords

Pien Tze HuangChinese medicinebreast cancerchemoresistanceepithelial-mesenchymal transition

references

More>

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Effect of Shengmai Yin on Epithelial-Mesenchymal Transition of Nasopharyngeal Carcinoma Radioresistant Cells

Related Author

No data

Related Institution

State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Science, Xiamen University

Fujian Pien Tze Huang Enterprise Key Laboratory of Natural Medicine Research and Development

Institute of Chinese Medicine, The Chinese University of Hong Kong

State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants, The Chinese University of Hong Kong

Department of Chemical Pathology, The Chinese University of Hong Kong

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰