Objective:

2

To explore the molecular-level mechanism on the hematopoiesis effect of

Angelicae sinensis Radix

(ASR) with systems-based interactome analysis.

Methods:

2

This systems-based interactome analysis was designed to enforce the workflow of "ASR (herb)→compound→target protein→internal protein actions→ending regulated protein for hematopoiesis". This workflow was deployed with restrictions on regulated proteins expresses in bone marrow and anemia disease and futher validated with experiments.

Results:

2

The hematopoiesis mechanism of ASR might be accomplished through regulating pathways of cell proliferation towards hemopoiesis with cross-talking agents of spleen tyrosine kinase (SYK)

Janus kinase 2 (JAK2)

and interleukin-2-inducible T-cell kinase (ITK). The hematopoietic function of ASR was also validated by colonyforming assay performed on mice bone marrow cells. As a result

SYK

JAK2 and ITK were activated.

Conclusion:

2

This study provides a new approach to systematically study and predict the therapeutic mechanism for ASR based on interactome analysis towards biological process with experimental validations.