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San-Ao Decoction (三拗汤) Regulates Urine Volume on Bronchial Asthma Model Mice
- Chinese Journal of Integrative Medicine Vol. 27, Issue 3, Pages: 212-219(2021)
- Affiliations：
School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing (100029), China
- Author bio：
Correspondence to: Prof. LI Yu-hang, E-mail: liyuhang@bucm.edu.cn
- Funds：
DOI：10.1007/s11655-018-3006-2
CLC：Published：01 March 2021，
Published Online：17 October 2018，
Accepted：19 January 2018
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Min WANG, Yan SUN, Shu-jing ZHANG, et al. San-Ao Decoction (三拗汤) Regulates Urine Volume on Bronchial Asthma Model Mice. [J]. Chinese Journal of Integrative Medicine 27(3):212-219(2021)
Min WANG, Yan SUN, Shu-jing ZHANG, et al. San-Ao Decoction (三拗汤) Regulates Urine Volume on Bronchial Asthma Model Mice. [J]. Chinese Journal of Integrative Medicine 27(3):212-219(2021) DOI： 10.1007/s11655-018-3006-2.
摘要
Abstract
Objective:
2
To observe the effect of San-Ao Decoction (三拗汤
SAD) on water metabolism of bronchial asthra model mice.
Methods:
2
Forty-five female BALB/c mice were randomly divided into control
model and SAD groups by a random number table
15 mice in each group. A composite method with ovalbumin (OVA) sensitization and challenge was developed to establish bronchial asthma model. Mice in the control group were intraperitoneally injected with distilled water without aerosol inhalation challenge. On day 15–22
0.3 mL SAD was administered via gastric route in SAD group
one time per day
while an equivalent volume of normal saline was used for gastric administration in the control and model groups. Changes in airway resistance in the inspiratory phase (RI-R-Area) were detected using an AniRes2005 system
and 5-h urine output was collected by metabolic cages. Histopathological changes in lung and kidney were observed by hematoxylin-eosin staining. mRNA expressions of aquaporin (AQP) 1 and AQP2 in kidney were detected by reverse transcription-polymerase chain reaction
and the protein expressions of AQP1 and AQP2 in kidney were detected by immunohistochemistry. Enzyme-linked immune sorbent assay was used to detect the OVA-specific endothelium-1 (ET-1)
antidiuretic hormone (ADH)
atrial natriuretic peptide (ANP)
prostaglandin E
2
(PGE
2
)
and angiotensin Ⅱ (Ang Ⅱ) levels in serum
lung and kidney tissues
respectively. The nitric oxide (NO) contents in serum
lung
and kidney tissues were tested by chemical method
respectively.
Results:
2
Compared with the control group
the serum IgE level in model group increased (
P
<
0.01). Following the pathologic changes in lung tissue
no significant change in kidney tissue was observed among 3 groups. Compared with the control group
the mice in the model group showed elevated airway resistance during inhalation phase
higher mRNA and protein expression levels on AQP1 and AQP2 in kidney tissue and higher ET-1 levels in serum
lung and kidney tissues
ADH and ANP in lung and serum
PGE
2
in kidney
Ang Ⅱ in lung and kidney tissues (
P
<
0.05 or
P
<
0.01)
but decreased in 5-h urinary output as well as NO and PGE
2
contents in serum and lung tissues (
P
<
0.05 or
P
<
0.01). Compared with the model group
the mice in the SAD group showed a weakened airway resistance in inspiratory phase
lower mRNA and protein expressions of AQP1 and AQP2 in kidney tissues
lower levels of ET-1
ADH
ANP in serum as well as ET-1
ANP
Ang Ⅱ levels in kidney tissues (
P
<
0.05 or
P
<
0.01)
whereas 5-h urinary output
NO content in kidney
ADH
ANP and PGE
2
levels in lung and Ang Ⅱ in serum increased (
P
<
0.05 or
P
<
0.01).
Conclusion:
2
San-Ao Decoction can regulate the urine volume through regulating AQP1 and AQP2 expression
and the expression of these in the kidneys might be regulated by ET-1
NO and Ang Ⅱ.
关键词
Keywords
regulation of fluid passageurine volumeaquaporinSan-Ao Decoctionbronchial asthma
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