Low-Frequency Electroacupuncture Alleviates Chronic Constrictive Injury-Induced Mechanical Allodynia by Inhibiting NR2B Upregulation in Ipsilateral Spinal Dorsal Horn in Rats

Wen-sheng ZHAO; Zhen-ni JIANG; Hui SHI; Lu-lu XU; Yue YANG; Ying-chao WANG

doi:10.1007/s11655-018-3057-4

Your Location：

Home >

Browse articles >

Low-Frequency Electroacupuncture Alleviates Chronic Constrictive Injury-Induced Mechanical Allodynia by Inhibiting NR2B Upregulation in Ipsilateral Spinal Dorsal Horn in Rats

Acupuncture Research | Updated：2021-08-27

- Low-Frequency Electroacupuncture Alleviates Chronic Constrictive Injury-Induced Mechanical Allodynia by Inhibiting NR2B Upregulation in Ipsilateral Spinal Dorsal Horn in Rats
- Chinese Journal of Integrative Medicine Vol. 25, Issue 6, Pages: 462-467(2019)
- Affiliations：
  
  1.Department of Pain Medicine, Hangzhou Red Cross Hospital, Hangzhou (310003), China
  2.Department of Cardiology, The Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou (310009), China
  3.Department of Acupuncture, The Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou (310009), China
  4.The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou (310053), China
  5.Department of Clinical Research Center, The Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou (310009), China
- Author bio：
  
  Correspondence to: Dr. JIANG Zhen-ni, Tel: 86-571-87784709, Fax: 86-571-87068001, E-mail: ze_jzn@zju.edu.cn
- Funds：
- DOI：10.1007/s11655-018-3057-4
  CLC：
- Published：01 June 2019，
  
  Published Online：22 November 2018，
  
  Accepted：08 June 2018
Scan for full text
Wen-sheng ZHAO, Zhen-ni JIANG, Hui SHI, et al. Low-Frequency Electroacupuncture Alleviates Chronic Constrictive Injury-Induced Mechanical Allodynia by Inhibiting NR2B Upregulation in Ipsilateral Spinal Dorsal Horn in Rats. [J]. Chinese Journal of Integrative Medicine 25(6):462-467(2019)
DOI：

Wen-sheng ZHAO, Zhen-ni JIANG, Hui SHI, et al. Low-Frequency Electroacupuncture Alleviates Chronic Constrictive Injury-Induced Mechanical Allodynia by Inhibiting NR2B Upregulation in Ipsilateral Spinal Dorsal Horn in Rats. [J]. Chinese Journal of Integrative Medicine 25(6):462-467(2019) DOI： 10.1007/s11655-018-3057-4.

摘要

Abstract

Objective:

To study the effects of electroacupuncture (EA) in chronic constrictive injury (CCI) rat model and the expression of N-methyl-D-aspartate receptor type 2B (NR2B) in ipsilateral spinal dorsal horn in rats to explore the analgesic mechanisms of EA.

Methods:

According to the random number table

totally 180 rats were evenly divided into a sham group

a CCI group

and an EA group. CCI model was conducted with four 4–0 chromic gut ligatures loosely ligated around the left sciatic nerve 1 cm above the trifurcation. Rats in the EA group received 2 Hz EA therapy bilaterally at acupoints of Zusanli (ST 36) and Sanyinjiao (SP 6) once daily (30 min/d) for 30 days after surgery. Paw withdrawal thresholds (PWTs) were measured on 0 (baseline)

30 days after surgery. Rats were sacrificed on 0

15 and 30 days after surgery

and the L4–5 segments of spinal cord were removed to detect the expression of NR2B by immunohistochemistry and quantitative polymerase chain reaction.

Results:

PWTs in the CCI group were significantly lower than the sham group at Day 1–30 after surgery

and reached its lowest at Day 1 (

0.01). After EA treatment

the PWTs recovered rapidly and were significantly higher than those in the CCI group on 3

15 and 30 days after surgery (

0.01). The numbers of NR2B-immunoreactive cells of the CCI group significantly increased after CCI surgery compared with the sham group (

0.01). Compared with the CCI group

stimulation of EA markedly decreased the numbers of NR2B-immunoreactive cells at Day 3

15 and 30 (

0.05). In the sham group

NR2B mRNA was expressed at a low level. It increased after CCI surgery

which increased rapidly at Day 7 (

0.01) and reached its peak value at Day 15 (

0.01). After EA stimulation

relative quantity of NR2B mRNA expression was less than that in the CCI group at Day 15 and 30 (

0.05).

Conclusions:

Low frequency of EA had antinociceptive effect in CCI rat model. The analgesic effects of EA might be through the inhibition of NR2B.

关键词

Keywords

sciaticaelectroacupuncturechronic constrictive injuryspinal dorsal hornN-methyl-D-aspartate receptor type 2BChinese medicine

references

More>

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Electroacupuncture Promotes Functional Recovery after Facial Nerve Injury in Rats by Regulating Autophagy via GDNF and PI3K/mTOR Signaling Pathway

Electroacupuncture Improves Blood-Brain Barrier and Hippocampal Neuroinflammation in SAMP8 Mice by Inhibiting HMGB1/TLR4 and RAGE/NADPH Signaling Pathways

Electroacupuncture Alleviates Functional Constipation in Mice by Activating Enteric Glial Cell Autophagy via PI3K/AKT/mTOR Signaling

Effect of Electro-acupuncture on Vasomotor Symptoms in Rats with Acute Cerebral Infarction Based on Phosphatidylinositol System

Related Author

No data

Related Institution

Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine

Department of Rehabilitation Medicine, Guanghan People's Hospital

Academic Affairs Office, Chengdu University of Traditional Chinese Medicine

College of Acu-moxibustion and Massage, Shaanxi University of Chinese Medicine

Department of Traditional Chinese Medicine, Baotou Medical College

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰