Objective:

2

To investigate the effects of

Ganoderma lucidum

polysaccharides (GL-PS) on human fibroblasts and skin wound healing in Kunming male mice and to explore the putative molecular mechanism.

Methods:

2

Primary human skin fibroblasts were cultured. The viability of fibroblasts treated with 0

10

20

40

80

and 160 μg/mL of GL-PS

respectively were detected by 3-4

5-dimethyl-2-thiazolyl-2

5-diphenyl-2-Htetrazolium bromide (MTT). The migration ability of fibroblasts treated with 0

10

20

and 40 μg/mL of GL-PS were measured by transwell assay. The secretion of the C-terminal peptide of procollagen type Ⅰ (CICP) and transforming growth factor-β1 (TGF-β1) in the cell supernatant was tested by enzyme-linked immunosorbent assay. The expression of β-catenin was detected by Western blot. Furthermore

the Kunming mouse model with full-layer skin resection trauma was established

and was treated with 10

20

and 40 mg/mL of GL-PS

respectively as external use. The size of the wound was measured daily

complete healing time in each group was recorded and the percentage of wound contraction was calculated.

Results:

2

Compared with the control group

10

20

and 40 μg/mL of GL-PS significantly increased the viability of fibroblasts

promoted the migration ability of fibroblasts

and up-regulated the expressions of CICP and TGF-β1 in fibroblasts (

P

<

0.05 or

P

<

0.01). The expression of β-catenin in fibroblasts treated with 20 and 40 μg/mL of GL-PS was significantly higher than that of the control group (

P

<

0.01). Furthermore

after external use of 10

20

and 40 mg/mL of GL-PS

the rates of wound healing in mice were significantly higher and the wound healing time was significantly less than the control group (

P

<

0.05 or

P

<

0.01).

Conclusion:

2

A certain concentration of GL-PS may promote wound healing via activation of the Wnt/β-catenin signaling pathway and up-regulation of TGF-β1

which might serve as a promising source of skin wound healing.