<italic style="font-style: italic">Humulus lupulus</italic> L. Extract Prevents Ovariectomy-Induced Osteoporosis in Mice and Regulates Activities of Osteoblasts and Osteoclasts

Tian-shuang XIA; Liu-yue LIN; Qiao-yan ZHANG; Yi-ping JIANG; Chang-hui LI; Xiao-yan LIU; Lu-ping QIN; Hai-liang XIN

doi:10.1007/s11655-019-2700-z

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Humulus lupulus L. Extract Prevents Ovariectomy-Induced Osteoporosis in Mice and Regulates Activities of Osteoblasts and Osteoclasts

Original Article | Updated：2021-08-16

- Humulus lupulus L. Extract Prevents Ovariectomy-Induced Osteoporosis in Mice and Regulates Activities of Osteoblasts and Osteoclasts
- Chinese Journal of Integrative Medicine Vol. 27, Issue 1, Pages: 31-38(2021)
- Affiliations：
  
  1.Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai (200433), China
  2.School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou (350108), China
  3.School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou (310053), China
- Author bio：
  
  Correspondence to: Prof. XIN Hai-liang, E-mail: hailiangxin@163.com
- Funds：
- DOI：10.1007/s11655-019-2700-z
  CLC：
- Published：01 January 2021，
  
  Published Online：27 March 2019，
  
  Accepted：31 August 2018
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Tian-shuang XIA, Liu-yue LIN, Qiao-yan ZHANG, et al. Humulus lupulus L. Extract Prevents Ovariectomy-Induced Osteoporosis in Mice and Regulates Activities of Osteoblasts and Osteoclasts. [J]. Chinese Journal of Integrative Medicine 27(1):31-38(2021)
DOI：

Tian-shuang XIA, Liu-yue LIN, Qiao-yan ZHANG, et al. Humulus lupulus L. Extract Prevents Ovariectomy-Induced Osteoporosis in Mice and Regulates Activities of Osteoblasts and Osteoclasts. [J]. Chinese Journal of Integrative Medicine 27(1):31-38(2021) DOI： 10.1007/s11655-019-2700-z.

摘要

Abstract

Objective:

To systematically evaluate the protective effects of

Humulus lupulus

L. extract (HLE) on osteoporosis mice.

Methods:

In vivo

experiment

a total of 35 12-week-old female ICR mice were equally divided into 5 groups: the sham control group (sham); the ovariectomy with vehicle group (OVX); the OVX with estradiol valerate [EV

0.2 mg/(kg•d)]; the OVX with low- or high-dose HLE groups [HLE

1 g/(kg•d) and 3 g/(kg•d)]

7 in each group. Treatment began 1 week after the ovariectomized surgery and lasted for 12 weeks. Bone mass and trabecular bone mircoarchitecture were evaluated by micro computed tomography

and bone turnover markers in serum were evaluated using enzyme-linked immunosorbent assay (ELISA) kits.

In vitro

experiment

osteoblasts and osteoclasts were treated with HLE at doses of 0

20 and 100 μg/mL. Biomarkers for bone formation in osteoblasts and bone resorption in osteoclasts were analyzed.

Results:

Compared with the OVX group

HLE exerted bone protective effects by the increase of estradiol (

0.05)

the improvement of cancellous bone structure

bone mineral density (

0.01) and the reduction of serum alkaline phosphatase (ALP)

tartrate resistant acid phosphatase (TRAP)

bone gla-protein

c-terminal telopeptides of type Ⅰ collagen (CTX-Ⅰ) and deoxypyridinoline levels (

0.01 for all).

In vitro

experiment

compared with the control group

HLE at 20 μg/mL promoted the cell proliferation (

0.01)

and increased the expression of bone morphogenetic protein-2 and osteopontin levels in osteoblasts (both

0.05). HLE at 100 μg/mL increased the osteoblastic ALP activities

and HLE at all dose enhanced the extracellular matrix mineralization (both

0.01). Furthermore

compared with the control group

HLE at 20 μg/mL and 100 μg/mL inhibited osteoclastic TRAP activity (

0.01)

and reduced the expression of matrix metalloproteinase-9 and cathepsin K (both

0.05).

Conclusion:

HLE may protect against bone loss

and have potentials in the treatment of osteoporosis.

关键词

Keywords

Humulus lupulus L.osteoporosisovariectomy-induced miceosteoblastsosteoclasts

references

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Humulus lupulus L. Extract Prevents Ovariectomy-Induced Osteoporosis in Mice and Regulates Activities of Osteoblasts and Osteoclasts

DOI：10.1007/s11655-019-2700-z

摘要

Abstract

关键词

Keywords

references