Objective:

2

To explore the ultrastructure characteristics of patients with dampness-heat of Pi (Spleen)-Wei (Stomach) syndrome (DHPW) and Pi-qi deficiency syndrome (PQD)

both of which are

Helicobacter pylori

(

Hp

)-correlated gastric diseases (HPCG)

and implicate a helpful hint for the clinical microcosmic syndrome differentiation.

Methods:

2

Fourteen gastric mucosa samples from 6 chronic gastritis (CG) and 6 active peptic ulcer (including 8 DHPW

4 PQD) as well as 2 healthy volunteers were collected and tested for

Hp

infection. The ultrastructure of gastric mucosa was observed under the transmission electron microscope (TEM).

Results:

2

Among 14 gastric mucosa samples

8 of them were

Hp

positive (6 DHPW and 2 PQD)

which were all accordance with the results screened by supermicro-pathological method. Under TEM

the normal gastric mucosa

with tidy microvilli and abundant in mucus granules

mitochondria and rough endoplasmic reticulum distributed evenly

and with smooth nucleus membrane. But in those specimens of DHPW with

Hp infection

microvilli were presented with burr shape. Especially

those samples from dampnessheat syndrome with predominant heat type (DHSH) patients were more obvious

with microvilli damaged

mitochondria concentrated and distributed in disorder

secretory tubule extended. In dampness-heat syndrome with predominant dampness type (DHSD) patients

mucus granules aggregated obviously

mitochondria swelled and blurred

and rough endoplasmic reticulum crowded. For 2 samples of DHPW without

Hp

infection

their microvilli were intact

with mitochondria increased and gathered but well-distributed

and secretory tubule extended mildly. In 2 PQD patients with

Hp

positive

the specimens of microvilli were sparse

and their mucus granules and mitochondria were decreased

with fractured crests and vacuole

secretory tubules extension to nucleus membrane

and rough endoplasmic reticulum extension in a pool-like way

and nucleus condensed. The 2 samples from PQD patients without

Hp

infection were characterized with intact microvilli

decreased mitochondria

fractured crest and extended rough endoplasmic reticulum in a pool-like way.

Conclusion:

2

It is obvious different in ultrastructure of DHPW and PQD patients under TEM

which may give a helpful hint for the microcosmic syndrome differentiation of HPCG.