<italic style="font-style: italic">Radix Kansui</italic> Stir-Fried with Vinegar Reduces <italic style="font-style: italic">Radix Kansui</italic>-Related Hepatotoxicity in Mice via Mitochondrial Pathway

Bei-hua BAO; Xiao-jing YAN; Yu-dan CAO; Wei-feng YAO; Fang-fang CHENG; Pei-dong CHEN; Ming-qiu SHAN; Li ZHANG; An-wei DING

doi:10.1007/s11655-019-3023-x

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Radix Kansui Stir-Fried with Vinegar Reduces Radix Kansui-Related Hepatotoxicity in Mice via Mitochondrial Pathway

Original Article | Updated：2021-08-16

- Radix Kansui Stir-Fried with Vinegar Reduces Radix Kansui-Related Hepatotoxicity in Mice via Mitochondrial Pathway
- Chinese Journal of Integrative Medicine Vol. 27, Issue 3, Pages: 192-197(2021)
- Affiliations：
  
  1.Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing (210023), China
  2.Menghe Medical Institute, Changzhou Traditional Chinese Medical Hospital Affiliated to Nanjing University of Chinese Medicine, Changzhou, Jiangsu Province (213003), China
- Author bio：
  
  Correspondence to: Prof. ZHANG Li, E-mail: zhangli@njucm.edu.cn
- Funds：
- DOI：10.1007/s11655-019-3023-x
  CLC：
- Published：01 March 2021，
  
  Published Online：7 May 2019，
  
  Accepted：30 July 2018
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Bei-hua BAO, Xiao-jing YAN, Yu-dan CAO, et al. Radix Kansui Stir-Fried with Vinegar Reduces Radix Kansui-Related Hepatotoxicity in Mice via Mitochondrial Pathway. [J]. Chinese Journal of Integrative Medicine 27(3):192-197(2021)
DOI：

Bei-hua BAO, Xiao-jing YAN, Yu-dan CAO, et al. Radix Kansui Stir-Fried with Vinegar Reduces Radix Kansui-Related Hepatotoxicity in Mice via Mitochondrial Pathway. [J]. Chinese Journal of Integrative Medicine 27(3):192-197(2021) DOI： 10.1007/s11655-019-3023-x.

摘要

Abstract

Objective:

To investigate the mechanism of

Radix Kansui

(RK) stir-fried with vinegar (VRK) decreased hepatotoxicity in mice.

Methods:

According to a random number table

40 mice were randomly divided into negative control group (0.5% carboxymethylcellulose sodium

20 mL/kg)

positive control group (0.1% mixture of carbon tetrachloride in soybean oil

20 mL/kg)

RK group (the ethyl acetate extracts of RK

250 g crude drug/kg) and VRK group (the ethyl acetate extracts of VRK

250 g crude drug/kg) with 10 mice per group. All mice were administered orally by gavage daily for 7 continuous days. The morphology of liver tissues was examined to assess the liver injury by a transmission electron microscope. Hepatocyte apoptosis

in vivo

was determined by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nickend labeling (TUNEL) assay. Immunohistochemical technique was adopted to detect the expression of particular antiapoptotic and proapoptotic proteins in the mitochondrial pathways

including B-cell lymphoma (Bcl-2) and caspase-3

as well as the expression of inflammatory mediators

including nuclear factor kappa B (NF-κB) and intercellular adhesion molecule-1 (ICAM-1).

Results:

Liver injury and hepatocyte apoptosis were observed in RK mice

and the liver injury were significantly reduced in VRK-treated mice. In immunohistochemistry study

compared with the negative control group

RK inhibited dramatically the Bcl-2 protein expression and significantly increased the expression of caspase-3

NF-κB and ICAM-1 (all

0.01). Compared with the RK group

VRK group induced significant increase on Bcl-2 protein expression

and decreased the caspase-3

NF-κB and ICAM-1 protein expression (

0.05 or

0.01).

Conclusion:

The mechanism of reduced hepatotoxicity of VRK may be associated with the reduced inflammation

regulation of antiapoptotic and proapoptotic mediators in the mitochondrial pathway.

关键词

Keywords

Radix Kansuimitochondrial pathwayhepatotoxicityapoptosisinflammationChinese medicine

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Department of Critical Care Medicine, Changzhou Hospital of Traditional of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine

Department of Critical Care Medicine, Jiangsu Province Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine

Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine

Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine

Department of Integrated Traditional Chinese and Western Medicine, Shanghai Skin Disease Hospital, Tongji University School of Medicine

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Radix Kansui Stir-Fried with Vinegar Reduces Radix Kansui-Related Hepatotoxicity in Mice via Mitochondrial Pathway

DOI：10.1007/s11655-019-3023-x

摘要

Abstract

关键词

Keywords

references