Danqi Tablet (丹七片) Regulates Energy Metabolism in Ischemic Heart Rat Model through AMPK/SIRT1-PGC-1α Pathway

Hui MENG; Qi-yan WANG; Ning LI; Hao HE; Wen-ji LU; Qi-xin WANG; Xiao-qian SUN; Shi-hong JIAO; Yong WANG; Peng-fei TU

doi:10.1007/s11655-019-3040-8

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Danqi Tablet (丹七片) Regulates Energy Metabolism in Ischemic Heart Rat Model through AMPK/SIRT1-PGC-1α Pathway

Original Article | Updated：2021-09-01

- Danqi Tablet (丹七片) Regulates Energy Metabolism in Ischemic Heart Rat Model through AMPK/SIRT1-PGC-1α Pathway
- Chinese Journal of Integrative Medicine Vol. 27, Issue 8, Pages: 597-603(2021)
- Affiliations：
  
  1.Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing(100029), China
  2.School of Life Sciences, Beijing University of Chinese Medicine, Beijing(100029), China
  3.College of Chinese Medicine, Beijing University of Chinese Medicine, Beijing(100029), China
- Author bio：
  
  Prof. TU Peng-fei, E-mail: pengfeitu@163.com
- Funds：
- DOI：10.1007/s11655-019-3040-8
  CLC：
- Published：01 August 2021，
  
  Published Online：30 May 2019，
  
  Accepted：23 January 2019
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Hui MENG, Qi-yan WANG, Ning LI, et al. Danqi Tablet (丹七片) Regulates Energy Metabolism in Ischemic Heart Rat Model through AMPK/SIRT1-PGC-1α Pathway. [J]. Chinese Journal of Integrative Medicine 27(8):597-603(2021)
DOI：

Hui MENG, Qi-yan WANG, Ning LI, et al. Danqi Tablet (丹七片) Regulates Energy Metabolism in Ischemic Heart Rat Model through AMPK/SIRT1-PGC-1α Pathway. [J]. Chinese Journal of Integrative Medicine 27(8):597-603(2021) DOI： 10.1007/s11655-019-3040-8.

摘要

Abstract

Objective:

To investigate the cardioprotective effect of Danqi Tablet (DQT

丹七片) on ischemic heart model rats and the regulative effect on energy metabolism through peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α).

Methods:

Rat ischemic heart model was induced by ligation of left anterior descending coronary artery. Totally 40 Sprague-Dawley rats were randomly divided into sham group

model group

DQT group (1.5 mg/kg daily) and trimetazidine (TMZ) group (6.3 mg/kg daily) according to a random number table

10 rats in each group. Twenty-eight days after continuous administration

cardiac function was assessed by echocardiography and the structures of myocardial cells were observed by hematoxylineosin staining. The level of adenosine triphosphate (ATP) in myocardial cells was measured by ATP assay kit. Expressions level of key transcriptional regulators

including PGC-1α

Sirtuin 1 (SIRT1)

AMP-activated protein kinase (AMPK)

and downstream targets of PGC-1α

such as mitofusin 1 (MFN1)

mitofusin 2 (MFN2) and superoxide dismutase 2 (SOD2) were measured by Western blot. Expression level of PGC-1α was examined by immunohistochemical staining.

Results:

The rat ischemic heart model was successfully induced and the heart function in model group was compromised. Compared with the model group

DQT exerted cardioprotective effects

up-regulated the ATP production in myocardial cells and inhibited the infiltration of inflammatory cells in the margin area of infarction of the myocardial tissues (

0.01). The expressions of PGC-1α

SIRT1 and AMPK were increased in the DQT group (all

0.05). Furthermore

the downstream targets

including MFN1

MFN2 and SOD2 were up-regulated (

0.05 or

0.01). Compared with the TMZ group

the expression levels of PGC-1α

MFN1 and SOD2 were increased by DQT treatment (

0.05 or

0.01).

Conclusions:

DQT regulated energy metabolism in rats with ischemic heart model through AMPK/SIRT1-PGC-1α pathway. PGC-1α might serve as a promising target in the treatment of ischemic heart disease.

关键词

Keywords

Danqi Tabletischemic heart diseaseenergy metabolismperoxisome proliferator-activated receptor-γ coactivator-1α

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Department of Traditional Chinese Medicine, China-Japan Friendship Hospital

Graduate Shcool, Beijing University of Chinese Medicine

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Center for Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences

Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing Key Laboratory of Pharmacology of Chinese Materia

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