Objective:

2

To study the effect of Liuwei Dihuang Decoction (六味地黄汤) or Yukmijihwangtang (YJT) on endurance exercise by

in vivo

experiment.

Methods:

2

ICR mice were randomly divided into the control group (distilled water) and the YJT groups (1

10

100 mg/kg)

5 animals per group. YJT and distilled water were orally administered. The anti-fatigue effect of YJT was evaluated by open filed test (OFT)

forced swimming test (FST)

and tail suspension test (TST).

Results:

2

In the OFT

YJT significantly increased the total movement distance in a dose-dependent manner. Additionally

treatment with YJT significantly decreased immobility time in the FST and the TST. Various neurotransmitters such as norepinephrine (NE)

serotonin (5-HT)

dopamine (DA) levels were increased by FST. Administration of YJT down-regulated the expression levels of NE

5-HT

5-hydroxyindole-acetic acid (5-HIAA)

and DA in the brain stem and hypothalamus of mice. Moreover

protein expression of HSP70 in mice liver and heart muscles was significantly increased in the YJT groups.

Conclusion:

2

YJT could ameliorate fatigue and enhance exercise tolerance through suppressing of brain monoamines including NE

5-HT

5-HIAA

and DA in FST mice model.