Objectives:

2

To investigate the effect on essential hypertension of the topical application of TAT-Cu

Zn-superoxide dismutase (TAT-SOD) at left acupoint Zusanli (ST 36)

and to observe whether the change of electrical potential difference (EPD) can be related to the change of blood pressure.

Methods:

2

Sixteen patients with essential hypertension and 16 healthy subjects were included in the study. EPD between the left acupoints of Yanglingquan (GB 34) and Qiuxu (GB 40) was firstly screened out for the EPD detection. An intracellular superoxide quenching enzyme

TAT-SOD

was topically applied to the acupoint ST 36 within an area of 1 cm

2

once a day

and the influence on EPD was investigated. The dosage applied to TAT-SOD group (

n

=8) was 0.2 mL of 3000 U/mL TAT-SOD cream prepared by adding purified TAT-SOD to a vehicle cream

while placebo group (

n

=8) used the vehicle cream instead. The left acupoints of Yanglingquan (GB 34) and Qiuxu (GB 40) were selected for EPD measurement after comparing EPD readings between 5 acupoints on each of all 12 meridians.

Results:

2

EPDs between the left acupoints of GB 34 and GB 40 for 16 patients of essential hypertension and 16 healthy subjects were 44.9±6.4 and 5.6±0.9 mV

respectively. Daily application of TAT-SOD for 15 days at ST 36 of essential hypertension patients significantly decreased systolic blood pressure (SBP) and diastolic blood pressure (DBP) of 179.6 and 81.5 mm Hg to 153.1 and 74.1 mm Hg

respectively. Responding to the change in blood pressure

EPD between the left acupoints of GB 34 and GB 40 also declined from 44.4 to 22.8 mV with the same trend. No change was observed with SBP

DBP and EPD between the left acupoints of GB 34 and GB 40 with the daily application of the placebo cream.

Conclusion:

2

Enzymatic scavenging of the intracellular superoxide at ST 36 proved to be effective in decreasing SBP and DBP. The results reconfirm the involvement of superoxide anions and its transportation along the meridians

and demonstrate that EPD between acupoints may be an indicator to reflect its functioning status. Moreover

preliminary results suggest a close correlation between EPD and blood pressure readings

implying a possibility of using EPD as a sensitive parameter for blood pressure and to monitor the effect of antihypertensive treatment.