Shexiang Tongxin Dropping Pill (麝香通心滴丸) Reduces Coronary Microembolization in Rats via Regulation of Mitochondrial Permeability Transition Pore Opening and AKT-GSK3β Phosphorylation

Yu DING; Hou-yong ZHU; Li-zong ZHANG; Bei-bei GAO; Liang ZHOU; Jin-yu HUANG

doi:10.1007/s11655-019-3176-6

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Shexiang Tongxin Dropping Pill (麝香通心滴丸) Reduces Coronary Microembolization in Rats via Regulation of Mitochondrial Permeability Transition Pore Opening and AKT-GSK3β Phosphorylation

Original Article | Updated：2021-09-01

- Shexiang Tongxin Dropping Pill (麝香通心滴丸) Reduces Coronary Microembolization in Rats via Regulation of Mitochondrial Permeability Transition Pore Opening and AKT-GSK3β Phosphorylation
- Chinese Journal of Integrative Medicine Vol. 27, Issue 7, Pages: 527-533(2021)
- Affiliations：
  
  1.Central Laboratory, Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou (310006), China
  2.Department of Cardiology, Hangzhou Chinese Medical Hospital, Hangzhou (310007), China
  3.Experimental Animal Research Center, Zhejiang Chinese Medical University, Hangzhou (310053), China
  4.Department of Cardiology, Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou (310006), China
- Author bio：
  
  Prof. HUANG Jin-yu, E-mail: hjyu41@sohu.com
- Funds：
- DOI：10.1007/s11655-019-3176-6
  CLC：
- Published：01 July 2021，
  
  Published Online：04 January 2020，
  
  Accepted：20 March 2019
Scan for full text
Yu DING, Hou-yong ZHU, Li-zong ZHANG, et al. Shexiang Tongxin Dropping Pill (麝香通心滴丸) Reduces Coronary Microembolization in Rats via Regulation of Mitochondrial Permeability Transition Pore Opening and AKT-GSK3β Phosphorylation. [J]. Chinese Journal of Integrative Medicine 27(7):527-533(2021)
DOI：

Yu DING, Hou-yong ZHU, Li-zong ZHANG, et al. Shexiang Tongxin Dropping Pill (麝香通心滴丸) Reduces Coronary Microembolization in Rats via Regulation of Mitochondrial Permeability Transition Pore Opening and AKT-GSK3β Phosphorylation. [J]. Chinese Journal of Integrative Medicine 27(7):527-533(2021) DOI： 10.1007/s11655-019-3176-6.

摘要

Abstract

Objective:

To investigate the protective effects of Shexiang Tongxin Dropping Pill (麝香通心滴丸

STDP) following sodium laurate-induced coronary microembolization (CME) in rats.

Methods:

Forty rats were divided into 4 groups: the control (sham) group

CME group

low-dose STDP pretreatment group (20 mg•kg

-1

•d

-1

)

and high-dose STDP pretreatment group (40 mg•kg

-1

•d

-1

). The rats were intragastric administrated with STDP 2 weeks before operation. Moreover

the histopathological alterations were observed using optical microscopy and transmission electron microscopy. Antioxidant biomarkers were analyzed by enzyme-linked immunosorbent assay. Mitochondrial functions including the mitochondrial permeability transition pore (mPTP) mtDNA copy number were determined and proteins of AKT/GSK3β were analyzed by Western blot.

Results:

The rats in the CME group showed a significant increase in the fibrinogen-like protein 2 expression level and mitochondrial dysfunction and a decrease in the expression level of antioxidant biomarkers (superoxide dismutase and catalase

0.01 for all). In contrast

the rats in the low- and high-dose STDP pretreatment groups showed a significant decrease in coronary microthrombi (

0.05); moreover

STDP restored the antioxidant-related protein activities and mitochondrial function

inhibited mPTP opening

decreased AKT-Ser473 phosphorylation

and increased GSK3β-Ser9 phosphorylation (

0.05 or

0.01).

Conclusion:

STDP may be useful for treatment of CME

possibly via regulation of mPTP opening and AKT/GSK3β phosphorylation.

关键词

Keywords

Shexiang Tongxin Dropping PillChinese medicinecoronary microembolizationmitochondrial permeability transition poreAKTGSK3β

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