Objective:

2

To study the protective mechanism of Chinese medicine Suxiao Jiuxin Pills (速效救心丸

SXJ) on myocardial ischemia and reperfusion (I/R) injury.

Methods:

2

Mouse myocardial I/R injury model was created by 30-min coronary artery occlusion followed by 24-h reperfusion

the mice were then divided into the sham group (

n

=7)

the I/R group (

n

=13)

the tirofiban group (TIR

positive drug treatment

n=9)

and the SXJ group (

n

=11). Infarct size (IS)

risk region (RR)

and left ventricle (LV) were analyzed with double staining methods. In addition

H9C2 rat cardiomyocytes were cultured with Na

2

S

2

O

4

to simulate I/R

in vitro

. The phosphorylation of extracellular regulated protein kinases1/2 (ERK1/2)

protein kinase B (AKT)

glycogen synthase kinase-3β (GSK3β)

and protein expression of GATA4 in nucleus were detected with Western blot assay.

Results:

2

The ratio of IS/RR in SXJ and TIR groups were lower than that in I/R group (SXJ

22.4%±6.6%; TIR

20.8%±3.3%; vs. I/R

35.4%±3.7%

P

<

0.05

respectively).

In vitro

experiments showed that SXJ increased the Na

2

S

2

O

4

-enhanced phosphorylation of AKT/GSK3β and nuclear expression of GATA4.

Conclusion:

2

SXJ prevents myocardial I/R injury in mice by activating AKT/GSK3β and GATA4 signaling pathways.