FOLLOWUS
Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid System
- Chinese Journal of Integrative Medicine Vol. 27, Issue 2, Pages: 98-105(2021)
- Affiliations：
1.Department of Cardiothoracic Surgery, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai(200437), China
2.Institute of Clinical Immunology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai (200437), China
3.Research Institute of Acupuncture Anesthesia, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai (201203), China
4.Department of Anesthesiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai (201203), China
- Author bio：
Correspondence to: Prof. CHEN Tong-yu, E-mail: tonychen080@sina.com
- Funds：
DOI：10.1007/s11655-020-3158-8
CLC：Published：01 February 2021，
Published Online：26 September 2020，
Accepted：05 November 2018
Scan for full text
Ke WANG, Yue YONG, Jia ZHOU, et al. Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid System. [J]. Chinese Journal of Integrative Medicine 27(2):98-105(2021)
Ke WANG, Yue YONG, Jia ZHOU, et al. Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid System. [J]. Chinese Journal of Integrative Medicine 27(2):98-105(2021) DOI： 10.1007/s11655-020-3158-8.
摘要
Abstract
Objective:
2
To investigate the action mechanisms of electroacupuncture (EA) on postoperative immunosuppression.
Methods:
2
Male C57BL/6 mice (5–7 weeks old) were randomly divided into: the sham injury group
the surgical trauma stressed group
the EA group [surgery + 2/100 Hz EA at Neiguan (PC 6)]
and the EA+ Nal (surgery + EA + intraperitoneal injection of naloxone). Abdominal surgical trauma stress mice model was established. EA was performed on bilateral PC 6 acupoints by an EA apparatus (2/100 Hz) for 20 min once a day for 3 days. The mRNA expressions of MOR
DOR
and KOR in thymus and L3–L5 dorsal root ganglions (DRG) were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and the protein expressions of MOR
DOR
and KOR in thymus were measured by Western blot. Flow cytometry assay was used to detect the levels of T lymphocyte subtypes in the peripheral blood.
Results:
2
Surgical trauma induced decreased the mRNA expression level of MOR in both thymus (
P
<
0.01) and L3–L5 DRGs (
P
<
0.05). Moreover
EA treatment not only significantly attenuated the MOR protein and mRNA expression in the thymus (both
P
<
0.05)
but also markedly increased expression of DOR and KOR opioid receptor in thymus (
P
<
0.01). However
the mRNA expressions of opioid receptors were not regulated by EA in the DRG (all
P
>
0.05). Furthermore
T lymphocyte population of CD
3
+
and CD
4
+
was decreased in the peripheral blood after surgical trauma (both
P
<
0.01). EA treatment can significantly elevate the population of CD
3
+
(
P
<
0.01)
CD
4
+
(
P
<
0.05) and CD
8
+
T cells (
P
<
0.01). Intraperitoneal injection of the non-selective opioid receptor antagonist naloxone blocked the up-regulation of T lymphocytes by EA.
Conclusion:
2
EA may improve postoperative immunosuppression through the peripheral opioid system.
关键词
Keywords
electroacupunctureChinese medicineimmunosuppressionopioid receptorT-lymphocytes
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