Opposite Modulatory Effects of <italic style="font-style: italic">Crataegus aronia</italic> Aqueous Extract on Platelet Aggregation in Rats

Abdullah S. Shatoor; Ali Shati; S. Al Humayed; Sultan AL-Qahtani; Mahmoud Alkhateeb

doi:10.1007/s11655-020-3187-3

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Opposite Modulatory Effects of Crataegus aronia Aqueous Extract on Platelet Aggregation in Rats

Original Article | Updated：2021-09-01

- Opposite Modulatory Effects of Crataegus aronia Aqueous Extract on Platelet Aggregation in Rats
- Chinese Journal of Integrative Medicine Vol. 27, Issue 9, Pages: 696-704(2021)
- Affiliations：
  
  1.Department of Medicine, Cardiology Section, College of Medicine, King Khalid University, Abha(64121), Saudi Arabia
  2.Department of Biology, College of Science, College of Medicine, King Khalid University, Abha(64121), Saudi Arabia
  3.Department of Physiology, College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh (11481), Saudi Arabia
- Author bio：
  
  Prof. Abdullah S. Shatoor, E-mail:asshalghamdi@yahoo.com
- Funds：
- DOI：10.1007/s11655-020-3187-3
  CLC：
- Published：01 September 2021，
  
  Published Online：16 May 2020，
  
  Accepted：13 May 2019
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Abdullah S. Shatoor, Ali Shati, S. Al Humayed, et al. Opposite Modulatory Effects of Crataegus aronia Aqueous Extract on Platelet Aggregation in Rats. [J]. Chinese Journal of Integrative Medicine 27(9):696-704(2021)
DOI：

Abdullah S. Shatoor, Ali Shati, S. Al Humayed, et al. Opposite Modulatory Effects of Crataegus aronia Aqueous Extract on Platelet Aggregation in Rats. [J]. Chinese Journal of Integrative Medicine 27(9):696-704(2021) DOI： 10.1007/s11655-020-3187-3.

摘要

Abstract

Objectives:

To reveal the mechanisms behind the dual effects of

Crataegus aronia

(

C. aronia

) aqueous extract on platelet aggregation by focusing on function

regulation

expression

and signaling of platelets P

receptors.

Methods:

Adult male Wistar rats (120±10 g) were classified as control received the vehicle

C. aronia

(200 mg/kg)

and

C. aronia

000 mg/kg)-treated rats. After treatments for consecutive 7 days

hematological and molecular experiments were conducted to detect alterations in platelet aggregation

thromboxane B2 (THXB2) and intracellular reactive oxygen species (ROS) content; protein levels of P

p-Akt

cyclic adenosine monophosphate (cAMP)

phosphorylated vasodilator-stimulated-phosphoprotein (p-VASP)

nuclear factor κB (NF-κB)

P-selectin

etc. in platelets were determined by Western blot; mRNA expressions of P

and some inflammatory markers were determined by real-time polymerase chain reaction.

Results:

At a concentration of 200 mg/kg

C. aronia inhibited platelet aggregation through multiple interconnected mechanisms including downregulation P

synthesis and expression

stimulating intracellular cAMP levels and protein levels of p-VASP

inhibiting platelets THXB2 release and protein levels of P-selectin. Also

it inhibited platelets level of ROS and of NF-κB

a major signaling pathway that stimulates the expression of P

and THXA2 synthesis. Opposite findings were seen in platelets of rats received

C. aronia

at a concentration of 2

000 mg/kg. Interestingly

co-administration of N-acetylcysteine prevented all hematological and molecular alterations exerted by the high dose of the extract and inhibited platelet aggregation.

Conclusion:

Oral administration of

C. aronia

at low dose inhibits platelet aggregation by reducing THXB2 release

expression of P-selectin and activating cAMP and Akt signaling through two major mechanisms including downregulation of P

and inhibition of ROS-induced activation of NF-κB

an effect that is observed to be in the opposite direction with its high dose.

关键词

Keywords

Crataegus aroniaplateletsaggregationP2Y12Intracellular cyclic adenosine monophosphatereactive oxygen speciesnuclear factor κB

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Sichuan Ant Recommendation Biotechnology Co., Ltd.

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Hunan Provincial Key Laboratory of the Research and Development of Novel Pharmaceutical Preparations, College of Pharmacy, Changsha Medical University

Sichuan Institute for Translational Chinese Medicine, Translational Chinese Medicine Key Laboratory of Sichuan Province, Sichuan Academy of Chinese Medicine Sciences

Department of Pharmacology, Southwest Medical University

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Opposite Modulatory Effects of Crataegus aronia Aqueous Extract on Platelet Aggregation in Rats

DOI：10.1007/s11655-020-3187-3

摘要

Abstract

关键词

Keywords

references