Effect of Modified Xijiao Dihuang Decoction (加味犀角地黄汤) on Intestinal Flora and Th17/Treg in Rats with Radiation Enteritis

Yu-guo WANG; Yue GAO; Jian FENG; Yong-qi DOU

doi:10.1007/s11655-020-3261-x

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Effect of Modified Xijiao Dihuang Decoction (加味犀角地黄汤) on Intestinal Flora and Th17/Treg in Rats with Radiation Enteritis

Original Article | Updated：2021-08-16

- Effect of Modified Xijiao Dihuang Decoction (加味犀角地黄汤) on Intestinal Flora and Th17/Treg in Rats with Radiation Enteritis
- Chinese Journal of Integrative Medicine Vol. 27, Issue 3, Pages: 198-205(2021)
- Affiliations：
  
  1.Medical School of Chinese People's Liberation Army, The General Hospital of People's Liberation Army, Beijing (100853), China
  2.Beijing Institute of Radiation Medicine, Beijing(100850), China
- Author bio：
  
  Correspondence to: Prof. DOU Yong-qi, E-mail: dyqi_301@yeah.net
- Funds：
- DOI：10.1007/s11655-020-3261-x
  CLC：
- Published：01 March 2021，
  
  Published Online：1 April 2020，
  
  Accepted：14 January 2020
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Yu-guo WANG, Yue GAO, Jian FENG, et al. Effect of Modified Xijiao Dihuang Decoction (加味犀角地黄汤) on Intestinal Flora and Th17/Treg in Rats with Radiation Enteritis. [J]. Chinese Journal of Integrative Medicine 27(3):198-205(2021)
DOI：

Yu-guo WANG, Yue GAO, Jian FENG, et al. Effect of Modified Xijiao Dihuang Decoction (加味犀角地黄汤) on Intestinal Flora and Th17/Treg in Rats with Radiation Enteritis. [J]. Chinese Journal of Integrative Medicine 27(3):198-205(2021) DOI： 10.1007/s11655-020-3261-x.

摘要

Abstract

Objective:

To observe the effect of Modified Xijiao Dihuang Decoction (加味犀角地黄汤

MXDD) on rats with radiation enteritis

and explore its action mechanism.

Methods:

Thirty female Sprague Dawley rats were divided into the control

model

dexamethasone (DXM)

golden bifid (GB) and MXDD groups using random number table

6 rats in each group. Except the control group

the other rats were developed into radiation enteritis model by exposing to a single

Co-γ ray at a dose of 11 Gy. The rats in the DXM

GB and MXDD groups were treated with DXM (1.425 mg/kg)

GB (0.8 g/kg) and MXDD (36.0 g/kg) once daily for 3 days

respectively. Body weight and diarrhea condition of rats were evaluated daily. On day 3

the feces of rats were collected for intestinal flora detection and the small intestinal tissues were also collected. Bacterial species annotation

alpha and beta diversities as well as composition of intestinal flora were detected and compared. The protein and mRNA expressions of interleukin 17 (IL-17)

retinoid-related orphan nuclear receptor gamma t (RORγt) and forkhead/ winged helix transcription factor p3 (FoxP3) were determined by Western blot and polymerase chain reaction

respectively. The abundance and diversity of intestinal flora as well as the proportion at the phylum and genus levels were assayed by 16S rRNA metagenome sequencing. Correlation between intestinal flora and Th17/Treg was analyzed by heatmap method.

Results:

On day 1 to 3 after radiation

compared with the control group

the body weight in model group was decreased (

0.05 or

0.01). Compared with the model group

MXDD could alleviate weight loss and diarrhea caused by irradiation. At the phylum level

MXDD cause a significant increase in

Firmicutes

and a decrease in

Proteobacteria

(

0.05 or

0.01). At the genus level

MXDD reduced the proportion of

Escherichia-Shigella

(

0.01). In addition

IL-17 and FoxP3 mRNA and protein expression levels were down-regulated and RORγt was up-regulated by MXDD treatment (

0.05). Besides

Firmicutes

and

Lactobacillus

were positively correlated with FoxP3 (

=0.73

0.79

respectively;

0.01)

negatively correlated with IL-17 (

=0.66

0.64

respectively;

0.01 or

0.05) and RORγt (

=0.73

0.81

respectively;

0.01).

Proteobacteria

and

Escherichia-Shigella

both had positive correlation with IL-17 (

=0.77

0.57

respectively;

0.01 or

0.05) and RORγt (

=0.94

0.79

respectively;

0.01) and negative correlation with FoxP3 (

=0.74

0.65;

0.01).

Conclusion:

MXDD could improve the survival status of irradiated rats by regulating the richness

diversity and composition of intestinal flora

and restoring the balance of Th17/Treg.

关键词

Keywords

Modified Xijiao Dihuang Decoctionradiation enteritisintestinal floraTh17/TregChinese medicine

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