Anti-tumor and Phenotypic Regulation Effect of Matrine on Dendritic Cells through Regulating TLRs Pathway

Jing-kang WANG; Bao-sheng ZHAO; Min WANG; Chen-yue LIU; Ya-qi LI; Quan-tao MA; Peng-fei LI; Tie-shan WANG; Chun-guo WANG; Yong-ming ZHOU

doi:10.1007/s11655-020-3433-8

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Anti-tumor and Phenotypic Regulation Effect of Matrine on Dendritic Cells through Regulating TLRs Pathway

Original Article | Updated：2021-09-01

- Anti-tumor and Phenotypic Regulation Effect of Matrine on Dendritic Cells through Regulating TLRs Pathway
- Chinese Journal of Integrative Medicine Vol. 27, Issue 7, Pages: 520-526(2021)
- Affiliations：
  
  1.School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing (100029), China
  2.Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing (100029), China
  3.School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing (100029), China
  4.Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing (100053), China
- Author bio：
  
  Prof. ZHOU Yong-ming, E-mail:zhoudr72@126.com
- Funds：
- DOI：10.1007/s11655-020-3433-8
  CLC：
- Published：01 July 2021，
  
  Published Online：10 November 2020，
  
  Accepted：18 June 2020
Scan for full text
Jing-kang WANG, Bao-sheng ZHAO, Min WANG, et al. Anti-tumor and Phenotypic Regulation Effect of Matrine on Dendritic Cells through Regulating TLRs Pathway. [J]. Chinese Journal of Integrative Medicine 27(7):520-526(2021)
DOI：

Jing-kang WANG, Bao-sheng ZHAO, Min WANG, et al. Anti-tumor and Phenotypic Regulation Effect of Matrine on Dendritic Cells through Regulating TLRs Pathway. [J]. Chinese Journal of Integrative Medicine 27(7):520-526(2021) DOI： 10.1007/s11655-020-3433-8.

摘要

Abstract

Objective:

To investigate the effects of matrine on antigen presentation of dendritic cells (DCs)

and to explore the pharmacological mechanism of matrine on anti-tumor effect.

Methods:

Different concentrations (0

8 and 16 μg/mL) of matrine were co-cultured with DCs

the harvested DCs were co-cultured with antigens of Lewis lung cancer (LLC) cells

and then DCs and T cells were co-cultured to produce DCs-activated killer (DAK) cells

which have significant tumor-killing activity. The expression of cytokines

mRNA and protein of toll-like receptors (TLRs) in DCs were detected by enzyme linked immunosobent assay

polymerase chain reaction and Western blot

respectively. And the killing effect of DAK were measured by MTT assay.

Results:

Matrine significantly increased the mRNA expression of TLR7

TLR8

myeloid differentiation factor 88 (MyD88)

tumor necrosis factor receptor-associated factor 6 (TRAF-6) and IκB kinase (IKK)

as well as the protein expression of TLR7 and TLR8

and up-regulated the levels of interleukin-12 (IL-12)

IL-6 and tumor necrosis factor-α (TNF-α)

meanwhile

it also increased the expressions of MHC-Ⅱ

CD54

CD80 and CD86 in DCs. DCs-activated effector T cells had significant tumor-killing activity. When the concentration of matrine was more than 4 μg/mL

all indices had significant difference (

0.01 or

0.05).

Conclusion:

Matrine plays an anti-tumor role by regulating TLRs signal transduction pathway

promoting the secretion of inflammatory cytokines and enhancing immune function.

关键词

Keywords

matrineChinese medicinedendritic cellstumorstoll-like receptorsinflammatory cytokines

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