Effects of Shenfu Injection (参附注射液) on Inflammatory Response during Post-Resuscitation Myocardial Dysfunction after Cardiac Arrest in Swine

Wei GU; Xiao-min HOU; Chun-sheng LI

doi:10.1007/s11655-021-2855-2

Your Location：

Home >

Browse articles >

Effects of Shenfu Injection (参附注射液) on Inflammatory Response during Post-Resuscitation Myocardial Dysfunction after Cardiac Arrest in Swine

Original Article | Updated：2021-09-01

- Effects of Shenfu Injection (参附注射液) on Inflammatory Response during Post-Resuscitation Myocardial Dysfunction after Cardiac Arrest in Swine
- Chinese Journal of Integrative Medicine Vol. 27, Issue 6, Pages: 417-423(2021)
- Affiliations：
  
  1.Department of Emergency Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation, Beijing (100050), China
  2.Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation, Beijing (100020), China
- Author bio：
  
  Prof. LI Chun-sheng, E-mail: lcscyyy@163.com
- Funds：
- DOI：10.1007/s11655-021-2855-2
  CLC：
- Published：01 June 2021，
  
  Published Online：09 January 2021，
  
  Accepted：29 May 2018
Scan for full text
Wei GU, Xiao-min HOU, Chun-sheng LI. Effects of Shenfu Injection (参附注射液) on Inflammatory Response during Post-Resuscitation Myocardial Dysfunction after Cardiac Arrest in Swine. [J]. Chinese Journal of Integrative Medicine 27(6):417-423(2021)
DOI：

Wei GU, Xiao-min HOU, Chun-sheng LI. Effects of Shenfu Injection (参附注射液) on Inflammatory Response during Post-Resuscitation Myocardial Dysfunction after Cardiac Arrest in Swine. [J]. Chinese Journal of Integrative Medicine 27(6):417-423(2021) DOI： 10.1007/s11655-021-2855-2.

摘要

Abstract

Objective:

To investigate whether Shenfu Injection (SFI

参附注射液) can alleviate post-resuscitation myocardial dysfunction by inhibiting the inflammatory response.

Methods:

After 8 min of ventricular fibrillation and 2 min of basic life support

24 pigs were randomly divided into 3 groups (n=8)

which were given intravenous bolus injections of SFI (1.0 mL/kg)

epinephrine (EP

0.02 mg/kg) and normal saline (SA)

respectively. The animals were sacrificed at 24 h after restoration of spontaneous circulation (ROSC)

and serum interleuking-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels were measured by enzyme-linked immunosorbent assay (ELISA); expressions of Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) mRNAs and proteins were determined by RT-PCR and Western blot

respectively.

Results:

Compared with the EP and the SA groups

the ultrastructure of myocardial cells were slightly damaged and the systolic function of the left ventricle was markedly improved in the SFI group at 24 h after ROSC (

0.05). In addition

compared with the EP and SA groups

the SFI group also showed significantly reduced levels of serum IL-6 and TNF-α

protein and mRNA levels of myocardial NF-κB and TLR4 (

0.05).

Conclusions:

Activation of TLR4/NF-κB signaling pathway may be involved in the pathological mechanisms of post-resuscitation myocardial dysfunction. SFI may block NF-κB-mediated inflammatory response by reducing the activity of NF-κB and the level of TNF-α

thus playing a protective role in post-resuscitation myocardial dysfunction.

关键词

Keywords

Shenfu InjectionChinese medicinecardiopulmonary resuscitationpost-resuscitation myocardial dysfunctionTLR4/NF-κB

references

WT Chang, MH Ma, KL Chien, CH Huang, MS Tsai, FY Shih, et al. Postresuscitation myocardial dysfunction:correlated factors and prognostic implications. Intensive Care Med 2007;33:88-95.

SP Chung, FQ Song, T Yu, Y Weng, S Sun, MH Weil, et al.Effect of therapeutic hypothermia vs delta-opioid receptor agonist on post resuscitation myocardialfunction in a rat model of CPR. Resuscitation 2011;82:350-354.

W Gu, CS Li, WP Yin, ZJ Guo, XM Hou, D Zhang.Apoptosis is involved in the mechanism of postresuscitation myocardial dysfunction in a porcine model of cardiac arrest.Am J Emerg Med 2012;30:2039-2045.

Y Huang, Q He. The relationship between sarcplasmic reticulum Ca2+ modulation proteins and postresuscitation myocardial dysfunction. Chin Critic Care Med (Chin)2014;26:697-700.

W Gu, CS Li, WP Yin, XM Hou, J Zhang, D Zhang, et al.Expression imbalance of transcription factors GATA-3 and T-bet in post-resuscitation myocardial immune dysfunction in a porcine model of cardiac arrest. Resuscitation 2013;84:848-853.

J Chen, H Jiang. TLR4 signal pathway and inflammation reaction. Med Recapitulate 2009;15:2902-2904.

W Gu, C Li, W Yin, Z Guo, X Hou, D Zhang. Shenfu Injection reduces postresuscitation myocardial dysfunction in a porcine model of cardiac arrest by modulating apoptosis. Shock 2012;38:301-306.

XF Ji, L Yang, MY Zhang, CS Li, S Wang, LH Cong. Shenfu Injection attenuates postresusbitation myocardial dysfunction in a porcine model of cardiac arrest. Shock 2011;35:530-536.

W Gu, C Li, W Yin, Z Guo, X Hou, D Zhang. Effects of different resuscitation modalities on post-resusciatation myocardial apoptosis in porcine models of cardiac arrest.Chin J Emerg Med (Chin) 2013;22(3):8-13.

AE Jones, NI Shapiro, JH Kilgannon, S Trzeciak. Goal-directed hemodynamic optimization in the post-cardiac arrest syndrome: a systematic review. Resuscitation 2008;77:26-29.

G Ma, GL Hong, GJ Zhao, MF Li, B Wu. Changes and significance of plasma B -type natriuretic peptide and cardiac troponin Ⅰ in patients with sepsis. Chin J Tradit Chin West Med Crit Care (Chin) 2014;21:99-103.

ZJ Liu, XL Liu, J Zhao, YJ Shi, LN Yan, XF Chen, et al. The effects of SOCS-1 on liver endotoxin tolerance development induced by a low dose of lipopolysaccharide are related to dampen NF-kappaB-mediated pathway. Dig Liver Dis 2008;40:568-577.

EE Varfolomeev, A Ashkenazi. Tumor necrosis factor: an apoptosis JuNKie? Cell 2004;116:491-497.

B Sun, H Fan, T Honda, R Fujimaki, A Lafond-Walker, Y Masui, et al. Activation of NF kappa B and expression of ICAM-1 in ischemic-reperfused canine myocardium. J Mol Cell Cardiol 2001;33:109-119.

W Gu, C Li, W Yin, Z Guo, X Hou, D Zhang, et al.Expression imbalance of transcription factors GATA-3 and T-bet is involved in the mechanism of postresuscitation lung injury. Chin J Emerg Med 2014;23:8-14.

K Xu, W Gu, XM Hou. Role of TLR4/NF-κB signal pathway in the pathogensis of acute myocardial dysfunction after resuscitation. Chin Critic Care Med (Chin) 2015;27:190-196.

J Cha, Z Wang, L Ao, N Zou, CA Dinarello, A Banerjee, et al. Cytokines link Toll-like receptor 4 signaling to cardiac dysfunction after global myocardial ischemia. Ann Thorac Surg 2008;85:1678-1685.

Y Zhang, CS Li, CJ Wu. Neuroprotective effect of Shenfu Injection following cardiac arrest in pig correlates with improved mitochondrialfunction and cerebral glucose uptake. Chin J Integr Med 2014;20:835-43.

XJ Zhang, L Song, ZG Zhou, XM Wang. Effect of Shenfu Injection on gastrointestinal microcirculation in rabbits after myocardial ischemia-reperfusion injury. World J Gastroenterol 2006;12:4389-4391.

X Hou, C Li, W Gu, Z Guo, W Yin, D Zhang. Effect of Shenfu on inflammatory cytokine release and brain edema after prolonged cardiac arrest in the swine. Am J Emerg Med 2013;31:1159-1164.

LQ Ze, PY Yi, Z Ning. Clinical efficacy of Shenfu Injection in treating severe sepsis and its effects on serum levels of interleukin-6 and interleukin-10. Chin Integr Tradit West Med Intens Critic (Chin) 2012;32:348-351.

W Gu, C Li, W Yin, X Hou. Effects of Shenfu Injection on the expression of transcription factors T-bet/GATA-3 in pigs with post-resuscitation myocardial dysfunction. Chin Critic Care Med 2015;27:190-196.

W Gu, Q Zhang, CS Li. Effects of Shenfu Injection on apoptosis of regulatory T lymphocytes in spleen during post-resuscitationimmune dysfunction ina porcine model of cardiac arrest. Chin J Integr Med 2016;22:666-73.

J Wang, LF Qiao, GT Yang. Role of Shenfu Injection in rats with systemic, inflammatory response syndrome. Chin J Integr Med 2008;14:51-55.

CD Zheng, S Min. Cardioprotection of Shenfu Injection against myocardial ischemia/reperfusion injury in open heart surgery. Chin J Integr Med 2008;14:10-16.

More>

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Therapeutic Effects of Shenfu Injection in Shock

Related Author

No data

Related Institution

Department of Emergency Medicine, Beijing Jishuitan Hospital

Department of Emergency Medicine, Critical Care Center, Capital Medical University Affiliated Beijing Friendship Hospital

Department of Emergency Medicine, Beijing Friendship Hospital, Capital Medical University

Heart Center & Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University

Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, Shaanxi University of Chinese Medicine

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰