Protective Effects of Hu-Lu-Ba-Wan (葫芦巴丸) against Oxidative Stress in Testis of Diabetic Rats through PKCα/NAPDH Oxidase Signaling Pathway

Shu-jun JIANG; Hui DONG; Ke FANG; Guang CHEN; Jing-bin LI; Li-jun XU; Xin ZOU; Fu-er LU

doi:10.1007/s11655-021-2863-2

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Protective Effects of Hu-Lu-Ba-Wan (葫芦巴丸) against Oxidative Stress in Testis of Diabetic Rats through PKCα/NAPDH Oxidase Signaling Pathway

Original Article | Updated：2021-09-01

- Protective Effects of Hu-Lu-Ba-Wan (葫芦巴丸) against Oxidative Stress in Testis of Diabetic Rats through PKCα/NAPDH Oxidase Signaling Pathway
- Chinese Journal of Integrative Medicine Vol. 27, Issue 6, Pages: 432-439(2021)
- Affiliations：
  
  1.Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan (430030), China
  2.Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan (430030), China
- Author bio：
  
  Prof. LU Fu-er, E-mail: felu@tjh.tjmu.edu.cn
- Funds：
- DOI：10.1007/s11655-021-2863-2
  CLC：
- Published：01 June 2021，
  
  Published Online：15 January 2021，
  
  Accepted：07 April 2020
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Shu-jun JIANG, Hui DONG, Ke FANG, et al. Protective Effects of Hu-Lu-Ba-Wan (葫芦巴丸) against Oxidative Stress in Testis of Diabetic Rats through PKCα/NAPDH Oxidase Signaling Pathway. [J]. Chinese Journal of Integrative Medicine 27(6):432-439(2021)
DOI：

Shu-jun JIANG, Hui DONG, Ke FANG, et al. Protective Effects of Hu-Lu-Ba-Wan (葫芦巴丸) against Oxidative Stress in Testis of Diabetic Rats through PKCα/NAPDH Oxidase Signaling Pathway. [J]. Chinese Journal of Integrative Medicine 27(6):432-439(2021) DOI： 10.1007/s11655-021-2863-2.

摘要

Abstract

Objective:

To explore the protective effect and the underlying mechanism of Hu-Lu-Ba-Wan (葫芦巴丸

HLBW) on the testis of diabetic rats.

Methods:

Twenty-four male Wistar rats (160–180 g) were randomly divided into 3 groups according to a random number table

including a control group (

=8)

diabetic group (

=8)

and HLBW group (

=8). Diabetic rat model was established by high-fat-diet administration and single intravenous injection of streptozotocin (26 mg/kg). Then HLBW granule was administrated for 12 weeks. Fasting blood glucose and insulin levels as well as serum total testosterone level and testicular testosterone content were examined. Oxidative stress markers in both serum and testis were tested. Meanwhile

testicular morphology was observed under hematoxylin and eosin (HE) and the ultrastructure of Leydig cell was observed by electron microscope. The superoxide anion level was detected by DHE

and TUNEL-positive cells of testis was evaluated by TUNEL assay. The gene and protein expression of protein kinase C (PKCα)

phosphorylated PKCα (P-PKCα) and P47phox in testicular tissues were determined by quantitative RT-PCR analysis and Western bolt analysis.

Results:

Compared with the diabetic group

HLBW treatment significantly reduced the fasting glucose levels and increased the levels of fasting insulin and testosterone in serum (

0.01). HLBW administration also reduced the levels of reactive oxygen species (ROS) in plasma and alleviated the damage of oxidative stress in the testis of diabetic rats. Additionally

HLBW down-regulated the protein and mRNA levels of PKCα

P-PKCα and P47phox in testicular tissues.

Conclusion:

HLBW may attenuate the oxidative stress in the testis of diabetic rats via PKCα /NAPDH oxidase signaling pathway.

关键词

Keywords

Hu-Lu-Ba-WanChinese medicineoxidative stresstestisdiabetesnicotinamide-adenine dinucleotide phosphate (NADPH) oxidase

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