Jiaotai Pill (交泰丸) Alleviates Insomnia through Regulating Monoamine and Organic Cation Transporters in Rats

Zhi-hui LI; Peng-kai MA; Yun-fang HUANG; Zhe ZHANG; Wei ZHENG; Jian-hua CHEN; Chang-e GUO; Ning CHEN; Xin-ning BI; Yu-jie ZHANG

doi:10.1007/s11655-021-3284-y

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Jiaotai Pill (交泰丸) Alleviates Insomnia through Regulating Monoamine and Organic Cation Transporters in Rats

Original Article | Updated：2021-08-16

- Jiaotai Pill (交泰丸) Alleviates Insomnia through Regulating Monoamine and Organic Cation Transporters in Rats
- Chinese Journal of Integrative Medicine Vol. 27, Issue 3, Pages: 183-191(2021)
- Affiliations：
  
  School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing (102488), China
- Author bio：
  
  Correspondence to: Prof. ZHANG Yu-jie, E-mail: zhyj227@126.com
- Funds：
- DOI：10.1007/s11655-021-3284-y
  CLC：
- Published：01 March 2021，
  
  Published Online：9 January 2021，
  
  Accepted：21 June 2020
Scan for full text
Zhi-hui LI, Peng-kai MA, Yun-fang HUANG, et al. Jiaotai Pill (交泰丸) Alleviates Insomnia through Regulating Monoamine and Organic Cation Transporters in Rats. [J]. Chinese Journal of Integrative Medicine 27(3):183-191(2021)
DOI：

Zhi-hui LI, Peng-kai MA, Yun-fang HUANG, et al. Jiaotai Pill (交泰丸) Alleviates Insomnia through Regulating Monoamine and Organic Cation Transporters in Rats. [J]. Chinese Journal of Integrative Medicine 27(3):183-191(2021) DOI： 10.1007/s11655-021-3284-y.

摘要

Abstract

Objective:

To reveal the effect and mechanism of Jiaotai Pill (交泰丸

JTP) on insomniac rats.

Methods:

The insomniac model was established by intraperitoneal injection of p-chlorophenylalanine (PCPA). In behavioral experiments

rats were divided into control

insomniac model

JTP [3.3 g/(kg•d)]

and diazepam [4 mg/(kg•d)]

groups. The treatment effect of JTP was evaluated by weight measurement (increasement of body weight)

open field test (number of crossings) and forced swimming test (immobility time). A high performance liquid chromatography–electrochemical detection (HPLC-ECD) method was built to determine the concentration of monoamine transmitters in hypothalamus and peripheral organs from normal

model

JTP

citalopram [30 mg/(kg•d)]

maprotiline [40 mg/(kg•d)]

and bupropion [40 mg/(kg•d)] groups. Expressions of serotonin transporter (SERT)

dopamine transporter (DAT)

and norepinephrine transporter (NET) were analyzed by quantitative polymerase chain reaction (qPCR) and Western blot in normal

model and JTP groups. A high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS/MS) method was established to determine the pharmacokinetics

urine cumulative excretion of metformin in vivo

and tissue slice uptake

in vitro

which were applied to assess the activity of organic cation transporters (OCTs) in hypothalamus and peripheral organs.

Results:

Compared with the insomniac model group

the body weight and spontaneous locomotor were increased

and the immobility time was decreased after treatment with JTP (

0.01). Both serotonin and dopamine contents in hypothalamus and peripheral organs were increased (

0.01). The norepinephrine content was increased in peripheral organs and decreased in hypothalamus (

0.05 or

0.01). At the same time

SERT

DAT

OCT1

OCT2

and OCT3 were down-regulated in hypothalamus and peripheral organs (

0.05). NET was down-regulated in peripheral organs and up-regulated in hypothalamus (

0.05 or

0.01). Moreover

the activity of OCTs in hypothalamus and peripheral organs was inhibited (

0.05).

Conclusion:

JTP alleviates insomnia through regulation of monoaminergic system and OCTs in hypothalamus and peripheral organs.

关键词

Keywords

Jiaotai Pillmonoaminergic systemmonoamine transmittershypothalamusperipheral organssleep disorderChinese medicine

references

Chen F, Chen G, Chen Z, Zhang Z, Zhang P, Luo D, et al. Guizhi Gancao Longgu Muli Decoction for insomnia:a protocol for a systematic review. Medicine (Baltimore)2020;99:e191-e198.

Baek JH, Nierenberg AA, Kinrys G. Clinical applications of herbal medicines for anxiety and insomnia; targeting patients with bipolar disorder. Aust N Z J Psychiatry 2014;48:705-715.

Anafi RC, Pellegrino R, Shockley KR, Romer M, Tufik S, Pack AI. Sleep is not just for the brain: transcriptional responses to sleep in peripheral tissues. BMC Genomics 2013;14:1-19.

van Neijenhof R, van Duijn E, van Den Berg JF, de Waal MWM, van der Mast RC, Comijs HC. Subjective insomnia symptoms and sleep duration are not related to hypothalamic–pituitary–adrenal axis activity in older adults. J Sleep Res 2018;27:40-46.

He W, Liu G, Cai H, Sun X, Hou W, Zhang P, et al. Integrated pharmacokinetics of five protoberberine-type alkaloids in normal and insomnic rats after single and multiple oral administration of Jiao-Tai-Wan. J Ethnopharmacol 2014;154:635-644.

Huang WY, Zou X, Lu FE, Zhang C, Ren YL, Xu LJ, et al.Effect of Jiaotai Pill on intestinal damage in partially sleep deprived rats. Chin J Integr Med 2017;23:901-907.

Qian Z, Wang SL, Tao WW, Long HY, Wang JW. Effects of Jiaotaiwan on depressive-like behavior in mice after lipopolysaccharide administration. Metab Brain Dis 2017;32:415-426.

Zou X, Huang W, Lu F, Fang K, Wang D, Zhao S, et al. The effects of Jiao-Tai-Wan on sleep, inflammation and insulin resistance in obesity-resistant rats with chronic partial sleep deprivation. BMC Complement Altern Med 2017;17:1-9.

Hirotsu C, Tufik S, Andersen ML. Interactions between sleep, stress, and metabolism: from physiological to pathological conditions. Sleep Sci 2015;8:143-152.

Happe S, Berger K, Investigators FS. The association of dopamine agonists with daytime sleepiness, sleep problems and quality of life in patients with Parkinson's disease-a prospective study. J Neurol 2001;248:1062-1067.

Yuan Q, Joiner WJ, Sehgal A. A sleep-promoting role for the Drosophila serotonin receptor 1A. Curr Biol 2006;16:1051-1062.

Mitchell HA, Weinshenker D. Good night and good luck:norepinephrine in sleep pharmacology. Biochem Pharmacol 2010;79:801-809.

Martin AM, Young RL, Leong L, Rogers GB, Spencer NJ, Jessup CF, et al. The diverse metabolic roles of peripheral serotonin. Endocrinology 2017;158:1049-1063.

Eisenhofer G. The role of neuronal and extraneuronal plasma membrane transporters in the inactivation of peripheral catecholamines. Pharmacol Ther 2001;91:35-62.

El-Merahbi R, Loffler M, Mayer A, Sumara G. The roles of peripheral serotonin in metabolic homeostasis. FEBS Lett 2015;589:1728-1734.

French IT, Muthusamy KA. A review of sleep and its disorders in patients with parkinson's disease in relation to various brain structures. Front Aging Neurosci 2016;8:1-14.

Cheng MH, Bahar I. Monoamine transporters: structure, intrinsic dynamics and allosteric regulation. Nat Struct Mol Biol 2019;26:545-556.

Andre P, Saubamea B, Cochois-Guegan V, Marie-Claire C, Cattelotte J, Smirnova M, et al. Transport of biogenic amine neurotransmitters at the mouse blood-retina and blood-brain barriers by uptake1 and uptake2. J Cereb Blood Flow Metab 2012;32:1989-2001.

Iversen L. Neurotransmitter transporters and their impact on the development of psychopharmacology. Br J Pharmacol 2006;147Suppl 1:S82-S88.

Bowman MA, Daws LC. Targeting serotonin transporters in the treatment of juvenile and adolescent depression. Front Neurosci 2019;13:156.

Imeri L, Bianchi S, Mancia M. Muramyl dipeptide and IL-1 effects on sleep and brain temperature after inhibition of serotonin synthesis. Am J Physiol 1997;273:R1663-1668.

Chen N, Guo CE, Chen H, Chen J, Bi X, Li H, et al.Simultaneous determination of 6 coptis alkaloids in urine and feces by LC-MS/MS and its application to excretion kinetics and the compatibility mechanism of Jiao-Tai-Wan in insomniac rats. Biomed Chromatogr 2018;32:e42-e48.

Gong MJ, Yue H, Zhou XY, Li CY, Wang SM, Li SW, et al. Mechanism of Jiaotai Pills in the intervention of PCPA–induced insomnia in the rats. World J Integr Tradit West Med (Chin) 2017;12:1230-1234.

Oh DR, Kim Y, Jo A, Choi EJ, Oh KN, Kim J, et al. Sedative and hypnotic effects of Vaccinium bracteatum Thunb.through the regulation of serotonegic and GABA-ergic systems: involvement of 5-HT receptor agonistic activity.Biomed Pharmacother 2019;109:2218-2227.

Gao X, Bi XN, Chen JH, Li HP, Huang YF, Qi J, et al.Changes of tissue distribution of seven Coptis Alkaloids in Jiaotai Pill in diabetic rats. Central South Pharm 2018;6:749-754.

Su J, Miao Q, Miao PP, Zhao YY, Zhang YY, Chen N, et al. Pharmacokinetics and brain distribution and metabolite identification of coptisine, a protoberberine alkaloid with therapeutic potential for CNS disorders, in rats. Biol Pharm Bull 2015;38:1518-1528.

Toni R. The neuroendocrine system: organization and homeostatic role. J Endocrinol Invest 2004;27:35-47.

Asikainen M, Toppila J, Alanko L, Ward DJ, Stenberg D, Porkka-Heiskanen T. Sleep deprivation increases brain serotonin turnover in the rat. Neuroreport 1997;8:1577-1582.

Bacq A, Balasse L, Biala G, Guiard B, Gardier AM, Schinkel A, et al. Organic cation transporter 2 controls brain norepinephrine and serotonin clearance and antidepressant response. Mol Psychiatry 2012;17:926-939.

Kliman HJ, Quaratella SB, Setaro AC, Siegman EC, Subha ZT, Tal R, et al. Pathway of maternal serotonin to the human embryo and fetus. Endocrinology 2018;159:1609-1629.

Daws LC. Unfaithful neurotransmitter transporters: focus on serotonin uptake and implications for antidepressant efficacy. Pharmacol Ther 2009;121:89-99.

Kim DK, Tolliver TJ, Huang SJ, Martin BJ, Andrews AM, Wichems C, et al. Altered serotonin synthesis, turnover and dynamic regulation in multiple brain regions of mice lacking the serotonin transporter. Neuropharmacology 2005;49:798-810.

Schonfeldt-Lecuona C, Kregel T, Schmidt A, Pinkhardt EH, Lauda F, Kassubek J, et al. From imaging the brain to imaging the retina: optical coherence tomography (OCT) in schizophrenia. Schizophr Bull 2016;42:9-14.

Tang M, Lei J, Sun X, Liu G, Zhao S. Stress-induced anhedonia correlates with lower hippocampal serotonin transporter protein expression. Brain Res 2013;1513:127-134.

Xing H, Zhang X, Xing N, Qu H, Zhang K. Uncovering pharmacological mechanisms of Zhi-Zi-Hou-Po Decoction in chronic unpredictable mild stress induced rats through pharmacokinetics, monoamine neurotransmitter and neurogenesis. J Ethnopharmacol 2019;243:1-8.

Kopin IJ, Rundqvist B, Friberg P, Lenders J, Goldstein DS, Eisenhofer G. Different relationships of spillover to release of norepinephrine in human heart, kidneys, and forearm.Am J Physiol 1998;275:R165-R173.

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