Mechanism of Shengmai Injection on Anti-Sepsis and Protective Activities of Intestinal Mucosal Barrier in Mice

Juan LU; Yue YU; Xiao-jing WANG; Rui-ping CHAI; Xin-kai LYU; Ming-hui DENG; Mei-geng HU; Yun QI; Xi CHEN

doi:10.1007/s11655-021-3292-y

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Mechanism of Shengmai Injection on Anti-Sepsis and Protective Activities of Intestinal Mucosal Barrier in Mice

Original Article | Updated：2022-09-08

- Mechanism of Shengmai Injection on Anti-Sepsis and Protective Activities of Intestinal Mucosal Barrier in Mice
- Chinese Journal of Integrative Medicine Vol. 28, Issue 9, Pages: 817-822(2022)
- Affiliations：
  
  Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing(100193), China
- Author bio：
  
  Prof. CHEN Xi, E-mail: chenx_implad@163.com
- Funds：
- DOI：10.1007/s11655-021-3292-y
  CLC：
- Published：2022-09，
  
  Published Online：09 July 2021，
  
  Accepted：11 June 2020
Scan for full text
Juan LU, Yue YU, Xiao-jing WANG, et al. Mechanism of Shengmai Injection on Anti-Sepsis and Protective Activities of Intestinal Mucosal Barrier in Mice. [J]. Chinese Journal of Integrative Medicine 28(9):817-822(2022)
DOI：

Juan LU, Yue YU, Xiao-jing WANG, et al. Mechanism of Shengmai Injection on Anti-Sepsis and Protective Activities of Intestinal Mucosal Barrier in Mice. [J]. Chinese Journal of Integrative Medicine 28(9):817-822(2022) DOI： 10.1007/s11655-021-3292-y.

摘要

Abstract

Objective:

To study the mechanism of Shengmai Injection (SMI) on anti-sepsis and protective activities of intestinal mucosal barrier.

Methods:

The contents of 11 active components of SMI including ginsenoside Rb1

Rb2

Rb3

Rg1

Rg2

ophioposide D

schisandrol A and schisantherin A were determined using ultra-performance liquid chromatography. Fifty mice were randomly divided into the blank

the model

the low-

medium- and high-dose SMI groups (0.375

0.75

1.5 mL/kg

respectively) by random number table

10 mice in each group. In SMI group

SMI was administrated to mice daily via tail vein injection for 3 consecutive days

while the mice in the blank and model groups were given 0.1 mL of normal saline. One hour after the last SMI administration

except the blank group

the mice in other groups were intraperitoneally injected with lipopolysaccharide (LPS) saline solution (2 mL/kg) at a dosage of 5 mL/kg for development of endotoxemia mice model. The mice in the blank group were given the same volume of normal saline. Inflammatory factors including interferon-γ (INF-γ)

tumor necrosis factor-α (TNF-α)

interleukin (IL)-2 and IL-10 were measured by flow cytometry. Myosin light-chain kinase (MLCK)

nuclear factor κB (NF-κB) levels

and change of Occludin proteins in jejunum samples were analyzed by Western blot.

Results:

The decreasing trends of INF-γ

TNF-α and IL-2 were found in serum of SMI treatment groups. In SMI-treated mice

the content of Occludin increased and MLCK protein decreased compared with the model group (

0.05 or

0.01). The content of cellular and nuclear NF-κB did not change significantly (

0.05).

Conclusion:

SMI may exert its anti-sepsis activity mainly through NF-κB-pro-inflammatory factor-MLCK-TJ cascade.

关键词

Keywords

Shengmai Injectionsepsisintestinal mucosal barriermechanismChinese medicine

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Department of Critical Care Medicine, Changzhou Hospital of Traditional of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine

Department of Critical Care Medicine, Jiangsu Province Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine

School of Chinese Medicine, Shandong University of Traditional Chinese Medicine

Research and Innovation Team of Emotional Diseases and Syndromes of Shandong University of Traditional Chinese Medicine

Key Laboratory of Traditional Chinese Medicine for Classical Theory, Ministry of Education

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