Influence of Acupuncture on Microcirculation Perfusion of Pericardium Meridian and Heart in Acute Myocardial Ischemia Model Rats

Yi ZHUANG; Jie ZHOU; Yu-mei ZHOU; Jiao CHEN; Ping WU; Pei-ran LYU; Min WAN; Liao-jun LUO; Ding-jun CAI; Fan-rong LIANG

doi:10.1007/s11655-021-3294-9

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Influence of Acupuncture on Microcirculation Perfusion of Pericardium Meridian and Heart in Acute Myocardial Ischemia Model Rats

Acupuncture Research | Updated：2022-03-18

- Influence of Acupuncture on Microcirculation Perfusion of Pericardium Meridian and Heart in Acute Myocardial Ischemia Model Rats
- Chinese Journal of Integrative Medicine Vol. 28, Issue 1, Pages: 69-75(2022)
- Affiliations：
  
  1.College of Acupuncture, Moxibustion and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu (610075),China
  2.College of Acupuncture, Moxibustion and Tuina,Nanjing University of Chinese Medicine, Nanjing (210023), China
  3.Department of Acupuncture and Moxibustion, Xin Du Hospital of Traditional Chinese Medicine, Chengdu (610599), China
  4.The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen ,Guangdong Province (518005), China
- Author bio：
- Funds：
- DOI：10.1007/s11655-021-3294-9
  CLC：
- Published：01 January 2022，
  
  Published Online：24 November 2021，
  
  Accepted：12 August 2020
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Yi ZHUANG, Jie ZHOU, Yu-mei ZHOU, et al. Influence of Acupuncture on Microcirculation Perfusion of Pericardium Meridian and Heart in Acute Myocardial Ischemia Model Rats. [J]. Chinese Journal of Integrative Medicine 28(1):69-75(2022)
DOI：

Yi ZHUANG, Jie ZHOU, Yu-mei ZHOU, et al. Influence of Acupuncture on Microcirculation Perfusion of Pericardium Meridian and Heart in Acute Myocardial Ischemia Model Rats. [J]. Chinese Journal of Integrative Medicine 28(1):69-75(2022) DOI： 10.1007/s11655-021-3294-9.

摘要

目的:

观察针刺对急性心肌缺血大鼠心包经和心脏微循环灌注量的影响

评价针刺是否能同时影响经络和相应脏器. 并通过比较针刺不同经络的穴位

讨论其是否具有相同作用.

方法:

采用结扎大鼠左前降支的方法建立急性心肌缺血模型. 将32只大鼠随机分为4组

急性心肌梗死组、针刺内关组、针刺列缺组及针刺前三里组

每组8只. 另8只大鼠仅接受开胸手术作为假手术组. 针刺组大鼠分别在内关、列缺和前三里穴进行手针干预15分钟. 采用激光散斑灌注成像仪 (LPSI) 监测针刺前后心包经和心脏的微循环灌注量

并用PSI系统计算和分析灌注单位 (PU) .

结果:

与针刺前比较

造模后急性心肌缺血组大鼠心脏微循环灌注量 (HMP) 在针刺期间 (

0.05) 和针刺后 (

0.05) 持续下降

心包经微循环灌注量 (PMP) 在3个阶段未见明显差异 (

0.05) . 与针刺前比较

内关组在针刺过程中PMP和HMP均明显升高

针刺后PMP明显降低 (均

0.05) ; 列缺组及前三里组PMP稍有升高

差异无统计学意义 (

0.05)

针刺干预后

上述指标明显降低 (

0.05) . 此外

前三里组HMP在针刺期间明显降低

尤其与针刺前比较

差异明显 (

0.05) .

结论:

针刺内关穴可明显提高急性心肌缺血模型大鼠心包经和心脏微循环灌注量

其作用优于列缺穴和前三里穴. 进一步证实针刺促进气血流注

并且具有穴位特异性与经络传播效应.

Abstract

Objective:

To observe the influence of acupuncture on microcirculation perfusion of the pericardium meridian and heart in acute myocardial ischemia (AMI) rats and evaluate whether acupuncture can simultaneously affect the meridians and corresponding viscera. Additionally

acupoints at different meridians were compared and whether they exert the same effects was discussed.

Methods:

Totally 32 Sprague-Dawley rats were subjected to left anterior descending (LAD) ligation to develop an AMI model. Rats were divided into 4 groups

including AMI

acupuncture Neiguan (PC 6)

Lieque (LU 7) and Qiansanli (LI 10) groups (

=8). Eight rats received only thoracotomy (sham-operated group). The rats in the acupuncture groups received manual acupuncture at PC 6

LU 7 and LI 10 acupoints for 15 min

respectively. The microcirculation perfusion of pericardium meridian and heart was monitored by laser speckle perfusion imager (LSPI) before

during and after acupuncture manipulation for 15 min. Subsequently

the perfusion unit (PU) was calculated and analyzed by PSI System.

Results:

After LAD

compared to pre-acupuncture stage

the heart microcirculation perfusion (HMP) in the AMI group decreased continuously at during-acupuncture (

0.05) and post-acupuncture stages (

0.05)

and the pericardium meridian microcirculation perfusion (PMP) showed no significant differences at 3 stages (

0.05). Compared to pre-acupuncture stage

the PMP and HMP in PC 6 group significantly increased during acupuncture manipulation (both

0.05)

and PMP decreased obviously after acupuncture (

0.05). The PMP in the LU 7 and LI 10 groups were slightly elevated (both

0.05); however

they were significantly reduced after acupuncture manipulation (both

0.05). Additionally

HMP of LI 10 group was decreased significantly during acupuncture

especially compared to pre-acupuncture stage (

0.05).

Conclusions:

Acupuncture at PC 6 obviously increased the PMP and HMP in AMI rats

and the effects were superior to at LU 7 and LI 10 acupoints. It was further confirmed that acupuncture promoted qi and blood circulation

indicating that acupoint specificity exists and features a meridian-propagated effect.

关键词

Keywords

microcirculation perfusionacupuncturepericardium meridianheartacute myocardial ischemia

references

Chan SH. What is being stimulated in acupuncture: evaluation of the existence of a specific substrate. Neurosci Biobehavioral Rev 1984;8:25-33.

Yang MN, Han JX. Review and analysis on the meridian research of China over the past sixty years. Chin J Integr Med 2015;21:394-400.

Tang Y, Yin HY, Liu J, Patrizia R, Peter I. P2X receptors and acupuncture analgesia. Brain Res Bull 2019;151:144-152.

Zhou YM, Zhuang Y, Cai DJ, Lv PR, Zhou J, Wan M, et al. Purines change at acupoints along the pericardium meridian in healthy and myocardial ischemic rats. Chin J Integr Med 2019;25:285-291.

Shu Q, Liang FX, Chen L, Liu JM, Wu S. Are oxygen and related compounds the mechanisms underlying acupoint function? a review of acupoint oxygen specificity studies. Complement Med Res 2016;23:336-343.

Khorsand A, Zhu J, Bahrami-Taghanaki H, Baghani S, Ma LX,Rezaei S. Investigation of the electrical impedance of acupuncture points and non-acupuncture points before and after acupuncture,using a four-electrode device. Acupunct Med 2015;33:230-236.

Huang T, Cheng X. The observation of the change of TCE caused by different acupuncture stimulation. Evid-Based Complement Altern Med 2013;2013:856905.

Peng C, Lin Y, Yang SQ, Gu CL, Ren J, Wang H, et al.Microcirculation changes of three yin-meridians of hand in the forearm in rabbits with arrhythmia: a comparative study. Chin Acupunct Moxibust (Chin) 2020;40:291-294.

Chen M, Wu ZX, Xu JS, Hu XL. Comparison of partial oxygen pressure and microcirculatory blood perfusion between acupoint and non-acupoint of large intestine channel. Chin J Tradit Chin Med Pharmacol (Chin) 2012;27:3198-3201.

Yang FM, Wang TH, Zhang HY, Zhou D, Xu ZF, Guo SJ, et al.Changes of the microcirculatory blood perfusion at "Feishu" (BL13) in the COPD rats. Chin Acupunct Moxibust (Chin) 2018;38:1303-1309.

Zhang WB, Wang LL, Xie HH, Li H, Tian YY. Comparison of acupuncture effect on blood perfusion between needling nonacupoint on meridian and needling nonacupoint off meridian. Evid-Based Complement Altern Med 2013;2013:426052.

Dai J, Ji B, Lu YW, Zhao GZ, Wang D, Yan MN, et al. Effect of electroacupuncture at "Neiguan" (PC 6) on skin microcirculation blood perfusion volume of heart-related acupoints and heart-irrelevant acupoints in myocardial ischemia rats. Acupunct Res 2017;42:434-438.

Strömberg T, Sjöberg F, Bergstrand S. Temporal, and spatiotemporal variability in comprehensive forearm skin microcirculation assessment during occlusion protocols. Microvascular Res 2017;113:50-55.

Treu C, Mdgc DS, Lupi O, Sicuro FL, Maranhão PA, Kraemer-Aguiar LG, et al. Structural and functional changes in the microcirculation of lepromatous leprosy patients-observation using orthogonal polarization spectral imaging and laser doppler flowmetry iontophoresis. PLoS One 2017;12:e0175743.

Zötterman J, Mirdell R, Horsten S, Farnebo S, Tesselaar E.Methodological concerns with laser speckle contrast imaging in clinical evaluation of microcirculation. PLoS One 2017;12:e0174703.

Cheng HY, Luo QM, Liu Q, Lu Q, Gong H, Zheng SQ. Laser speckle imaging of blood flow in microcirculation. Physics Med Bio 2004;49:1347-1357.

Mitrou N, Scully CG, Braam B, Chon KH, Cupples WA. Laser speckle contrast imaging reveals large-scale synchronization of cortical autoregulation dynamics influenced by nitric oxide. Am J Physiol Renal Physiol 2015;308:661-670.

Kojima S, Sakamoto T, Nagai Y, Matsui Y, Nambu K, Masamune K. Laser speckle contrast imaging for intraoperative quantitative assessment of intestinal blood perfusion during colorectal surgery: a prospective pilot study. Surg Innov 2019;26:293-301.

Kanda H, Kunisawa T, Iida T, Tada M, Kimura F, Ise H, et al. Cerebral circulation during retrograde cerebral perfusion: evaluation using laser speckle flowgraphy. Ann Thorac Surg 2019;107:1747-1752.

Ding N, Jiang J, Liu XX, Xu YY, Hu JT, Li ZG. Laser speckle imaging of sensitized acupoints. Evid-Based Complement Altern Med 2018;2018:7308767.

Yu SG, Xu B, eds. Experimental acupuncture. Beijing: People's Medical Publishing House; 2018:287-290.

Jiang YF, Chen CS, Liu XM, Liu RT, Liu SH. Light propagation along the pericardium meridian at human wrist as evidenced by the optical experiment and Monte Carlo method. Chin J Integr Med 2015;21:254-258.

Guo Y, Zhang YJ, Wang XY, Xu TP, Chen JS, Zhang CX, et al. Study on the correlation between calcium ions and meridian acupoints. World Chin Med (Chin) 2020;25:970-975.

Ahn AC, Colbert AP, Anderson BJ, Martinsen OG, Hammerschlag R,Cina S, et al. Electrical properties of acupuncture points and meridians:a systematic review. Bioelectromagnetics 2008;29:245-256.

Yang MX, Sun MS, Du T, Long HL, Chen J, Liang FR, et al. The efficacy of acupuncture for stable angina pectoris: a systematic review and meta-analysis. Eur J Prevent Cardiol 2019;17:2047487319876761.

Zhao L, Li DH, Zheng H, Chang XR, Cui J, Wang RH, et al. Acupuncture as adjunctive therapy for chronic stable angina: a randomized clinical trial. J Am Med Assoc Intern Med 2019;179:1388-1397.

Patel MB, Bui LP, Kirkeeide RL, Gould KL. Imaging microvascular dysfunction and mechanisms for female-male differences in CAD. J Am Coll Cardiol Cardiovascular Image 2016;9:465-482.

Naja DM, Marie MM, Adam P, Daria F, Nynne D, Ahmed A, et al.Coronary microvascular function, and cardiovascular risk factors in women with angina pectoris and no obstructive coronary artery disease: the iPOWER study. J Am Heart Association 2016;5:e003064.

Sorelle R. Microcirculatory function in postmenopausal women and the effect of long-term and short-term estrogen administration.Circulation 2002;105:9069-9070.

Lei Q, Chen C, Wu XL, Chen WJ, Yi T, Ma MD, et al. Dynamic detection of surface blood flow in rat heart and its application in real time identification of myocardial infarction model. Nat Med J Chin(Chin) 2017;97:1019-1023.

Zhang D, Song XJ, Li SY, Wang SY, Chen BJ, Bai XD, et al.Evaluation of liver function and electroacupuncture efficacy of animals with alcoholic liver injury by the novel imaging methods. Sci Rep 2016;6:30119.

Huang T, Zhang WB, Jia SY, Tian YY, Wang GJ, Yang LJ, et al.A transcontinental pilot study for acupuncture lifting-thrusting and twisting-rotating manipulations. Evid-Based Complement Altern Med 2012;2012:157989.

Liu Q, Li Y, Lu HY, Tong SB. Real-time high resolution laser speckle imaging of cerebral vascular changes in a rodent photothrombosis model. Biomed Optics Exp 2014;5:1483-1493.

Song XJ, Zhang D, Wang SY, Li SY. Investigation of hepatic blood perfusion by laser speckle imaging and changes of hepatic vasoactive substances in mice after electroacupuncture. Evid-Based Complement Altern Med 2014;2014:715316.

Ma SX, Mayer E, Lee P, Li XY, Gao EZ. Transcutaneous electrical stimulation increased nitric oxide-cyclic GMP release biocaptured over skin surface of pericardium meridian and acupuncture points in humans. Acupunct Electro-Therap Res 2015;40:73-86.

Zheng SX, Pan XH, Xu JS, Xiu CY, Dong YQ, Zhu XX.Variations in energy metabolism along the pericardium meridian and its relationship with visceral function adjustments during electroacupuncture. BMC Complement Altern Med 2014;14:323.

Zhao L, Chen J, Li Y, Sun X, Chang XR, Zheng H, et al. The long-term effect of acupuncture for migraine prophylaxis: a randomized clinical trial. J Am Med Association Intern Med 2017;177:508-515.

Zheng H, Li J, Li Y, Zhao L, Wu X, Chen J, et al. Acupuncture for patients with mild hypertension: a randomized controlled trial. J Clin Hypertens (Greenwich) 2019;21:412-420.

Zhang QQ, Zhu SP, Luo L, Guo MW, Zhang L, Shen SX, et al. Effect of different acupuncture stimuli on uterine micro-circulation on circulation-related substances of dysmenorrheal rats with cold stagnation syndrome. Chin J Inform Tradit Chin Med (Chin) 2015;22:51-55.

Min S, Lee H, Kim SY, Park JY, Chae YB, Lee HJ, et al. Local changes in microcirculation and the analgesic effects of acupuncture:a laser doppler perfusion imaging study. J Altern Complement Med 2015;21:46-52.

Zhang WB, Wang LL, Huang T, Tian YY, Xu YH, Wang L,et al. Laser doppler perfusion imaging for assessment of skin blood perfusion after acupuncture. Med Acup 2008;20:109-118.

Sandberg M, Larsson B, Lindberg LG, Gerdle B. Different patterns of blood flow response in the trapezius muscle following needle stimulation (acupuncture) between healthy subjects and patients with fibromyalgia and work-related trapezius myalgia. Eur J Pain 2005;9:497-510.

Tsuchiya M, Sato EF, Inoue M, Asada A. Acupuncture enhances generation of nitric oxide and increases local circulation. Anesthesia Analgesia 2007;104:301-307.

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China Academy of Chinese Medical Sciences

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