Protective Effect of <italic style="font-style: italic">Brassica napus</italic> L. Hydrosols against Inflammation Response in RAW 264.7 Cells

Cho Su-Hyeon; Rae Kim Song; Seon Jeong Myeong; Choi Miri; Park SeonJu; Kim Kil-Nam

doi:10.1007/s11655-021-3330-9

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Protective Effect of Brassica napus L. Hydrosols against Inflammation Response in RAW 264.7 Cells

Original Article | Updated：2021-08-16

- Protective Effect of Brassica napus L. Hydrosols against Inflammation Response in RAW 264.7 Cells
- Chinese Journal of Integrative Medicine Vol. 27, Issue 4, Pages: 273-279(2021)
- Affiliations：
  
  Chuncheon Center, Korea Basic Science Institute, Chuncheon(24341), Republic of Korea
- Author bio：
  
  Correspondence to: Dr. Kil-Nam Kim, E-mail: knkim@kbsi.re.kr
- Funds：
- DOI：10.1007/s11655-021-3330-9
  CLC：
- Published：01 April 2021，
  
  Accepted：27 December 2019
Scan for full text
Cho Su-Hyeon, Rae Kim Song, Seon Jeong Myeong, et al. Protective Effect of Brassica napus L. Hydrosols against Inflammation Response in RAW 264.7 Cells. [J]. Chinese Journal of Integrative Medicine 27(4):273-279(2021)
DOI：

Cho Su-Hyeon, Rae Kim Song, Seon Jeong Myeong, et al. Protective Effect of Brassica napus L. Hydrosols against Inflammation Response in RAW 264.7 Cells. [J]. Chinese Journal of Integrative Medicine 27(4):273-279(2021) DOI： 10.1007/s11655-021-3330-9.

摘要

Abstract

Objective:

To demonstrate the anti-inflammatory activity of Brassica napus L. hydrosols (BNH) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.

Methods:

Composition analysis of BNH was conducted via gas chromatography-mass spectrometry after BNH were extracted. The nitric oxide (NO) production was measured using the Griess assay. Prostaglandin E

(PGE

) production was evaluated with enzyme-linked immunosorbent assay. The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis. Furthermore

phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining

respectively.

Results:

Compared with LPS-stimulated cells

BNH markedly decreased the generation of NO and PGE

in LPS-stimulated RAW 264.7 cells (

0.01 or

0.05). Moreover

BNH inhibited protein levels of iNOS and COX-2 (

0.01). Phosphorylation of NF-κB and nuclear translocation of NF-κB p65 was significantly inhibited by BNH (

0.01 or

0.05).

Conclusion:

The anti-inflammatory activities of BNH were mediated via blockage of the NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells.

关键词

Keywords

Brassica napus L.inflammationlipopolysaccharideRAW 264.7 cellshydrosols

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Clinical Pharmacokinetic Laboratory, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

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Department of Integrated Traditional Chinese and Western Medicine, Shanghai Skin Disease Hospital, Tongji University School of Medicine

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Protective Effect of Brassica napus L. Hydrosols against Inflammation Response in RAW 264.7 Cells

DOI：10.1007/s11655-021-3330-9

摘要

Abstract

关键词

Keywords

references