Electroacupuncture Attenuates Ischemic Brain Injury and Cellular Apoptosis via Mitochondrial Translocation of Cofilin

Bin CHEN; Wan-qing LIN; Zuan-fang LI; Xiao-yong ZHONG; Jing WANG; Xiao-fang YOU; Hong-jia ZHAO; Da-shi QI

doi:10.1007/s11655-021-3335-4

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Electroacupuncture Attenuates Ischemic Brain Injury and Cellular Apoptosis via Mitochondrial Translocation of Cofilin

Acupuncture Research | Updated：2021-09-01

- Electroacupuncture Attenuates Ischemic Brain Injury and Cellular Apoptosis via Mitochondrial Translocation of Cofilin
- Chinese Journal of Integrative Medicine Vol. 27, Issue 9, Pages: 705-712(2021)
- Affiliations：
  
  1.Department of Rehabilitation Medicine and National Clinical Research Base of Traditional Chinese Medicine, the Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou(350004), China
  2.Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou (350108), China
  3.Shanghai Key Lab of Birth Defect, Children's Hospital of Fudan University, Shanghai (200032), China
- Author bio：
  
  Prof. QI Da-shi, E-mail: qidashi@126.com
- Funds：
- DOI：10.1007/s11655-021-3335-4
  CLC：
- Published：01 September 2021，
  
  Published Online：12 March 2021，
  
  Accepted：07 February 2021
Scan for full text
Bin CHEN, Wan-qing LIN, Zuan-fang LI, et al. Electroacupuncture Attenuates Ischemic Brain Injury and Cellular Apoptosis via Mitochondrial Translocation of Cofilin. [J]. Chinese Journal of Integrative Medicine 27(9):705-712(2021)
DOI：

Bin CHEN, Wan-qing LIN, Zuan-fang LI, et al. Electroacupuncture Attenuates Ischemic Brain Injury and Cellular Apoptosis via Mitochondrial Translocation of Cofilin. [J]. Chinese Journal of Integrative Medicine 27(9):705-712(2021) DOI： 10.1007/s11655-021-3335-4.

摘要

Abstract

Objective:

To investigate the potential mechanisms of electroacupuncture (EA) to prevent ischemic stroke.

Methods:

The method of middle cerebral artery occlusion (MCAO) was employed to establish a rat model of ischemic stroke. Seventy-eight Sprague-Dawley rats were divided into the sham group

MCAO + EA control (EC) group

and MCAO + EA (EA) group according to a random number table (

=26 per group). EA was applied to the acupoints of Baihui (DU 20) and Shenting (DU 24) 5 min and 6 h

respectively after the onset of MCAO for 30 min. Rats in the sham and EC groups received only light isoflurane anesthesia for 30 min after MCAO. The neuroprotective effects of EA were evaluated by rota-rod test

neurological deficit scores and infarct volumes. Additionally

Nissl staining and immunostaining were performed to examine brain damage

rod formation

cellular apoptosis

and neuronal loss induced by ischemia. The activities of caspase-3

and expression levels of cofilin and p-cofilin in mitochondria and cytoplasm after ischemic injury were determined by Western blot.

Results:

Compared with the EC group

EA significantly improved neuromotor function and cognitive ability after ischemic stroke (

0.05 or

0.01). Therapeutic use of EA also resulted in a significant decrease of cofilin rod formation and microtubule-associated protein-2 (MAP2) degradation in the cortical penumbra area compared with the EC rats (

0.01). Furthermore

Western blot analysis showed that EA stimulation significantly inhibited mitochondrial translocation of cofilin and caspase-3 cleavage (

0.05 or

0.01). Additionally

brain damage (infarct volume and neuropathy)

cellular apoptosis and neuronal loss induced by ischemia were remarkably suppressed by EA in the cortical penumbra of rats (

0.05 or

0.01).

Conclusion:

EA treatment after ischemic stroke may attenuate ischemic brain injury and cellular apoptosis through the regulation of mitochondrial translocation of cofilin

a novel mechanism of EA therapy.

关键词

Keywords

electroacupuncturecofilinapoptosisischemic strokemitochondrial translocationChinese medicine

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