Antitumor Effects of Ethanol Extract from <italic style="font-style: italic">Ventilago leiocarpa</italic> Benth on Sarcoma 180 Tumor-Bearing Mice and Possible Immune Mechanism<sup>*</sup>

Dao-Hai CHENG; Ying LIU; Li WANG

doi:10.1007/s11655-021-3440-4

Your Location：

Home >

Browse articles >

Antitumor Effects of Ethanol Extract from Ventilago leiocarpa Benth on Sarcoma 180 Tumor-Bearing Mice and Possible Immune Mechanism^*

Original Article | Updated：2021-11-29

- Antitumor Effects of Ethanol Extract from Ventilago leiocarpa Benth on Sarcoma 180 Tumor-Bearing Mice and Possible Immune Mechanism^*
- Chinese Journal of Integrative Medicine Vol. 27, Issue 12, Pages: 905-911(2021)
- Affiliations：
  
  1.Department of Pharmacy, First Affiliated Hospital of Guangxi Medical University, Nanning (530021), China
  2.Department of Pharmacology, Guangxi Institute of Chinese Medicine &Pharmaceutical Science, Nanning (530022), China
  3.Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Nanning (530022), China
- Author bio：
  
  Dr. LIU Ying, E-mail: shenghuakai_1997@163.com
- Funds：
- DOI：10.1007/s11655-021-3440-4
  CLC：
- Published：15 November 2021，
  
  Published Online：30 January 2021，
  
  Accepted：06 November 2020
Scan for full text
Dao-Hai CHENG, Ying LIU, Li WANG. Antitumor Effects of Ethanol Extract from Ventilago leiocarpa Benth on Sarcoma 180 Tumor-Bearing Mice and Possible Immune Mechanism^*. [J]. Chinese Journal of Integrative Medicine 27(12):905-911(2021)
DOI：

Dao-Hai CHENG, Ying LIU, Li WANG. Antitumor Effects of Ethanol Extract from Ventilago leiocarpa Benth on Sarcoma 180 Tumor-Bearing Mice and Possible Immune Mechanism^*. [J]. Chinese Journal of Integrative Medicine 27(12):905-911(2021) DOI： 10.1007/s11655-021-3440-4.

摘要

目的:

探讨翼核果醇提物 (ethanol extract from Ventilago leiocarpa Benth

EEVLB) 对荷瘤小鼠S180的抗肿瘤作用及其可能的机制.

方法:

将60只小鼠按随机数字表法随机分为6组: 正常组、模型组、5-氟尿嘧啶 (5-FU) 组 (0.02 g·kg-1) 和EEVLB高、中、低剂量组 (生药100、84、56 g·kg-1)

每组10只. 每天给药1次

连续10 天. 通过检测各组小鼠肿瘤抑制率、脏器指数、血清白细胞介素 (IL) -2、-6、-10、CD3

CD4

T淋巴细胞、CD4

/CD8

比值、caspase-3、Bcl-2水平

评价EEVLB对肿瘤生长和免疫功能的影响.

结果:

不同浓度的EEVLB均能在体内抑制肿瘤生长

其中高剂量组瘤重显著下降

肿瘤细胞凋亡明显增加 (

0.05). 此外

EEVLB处理组小鼠净增重和脾脏指数均明显升高 (

0.05). 与模型组比较

EEVLB高、中剂量组血清白介素 (IL) -2、IL-6水平、外周血CD3

CD4

T淋巴细胞百分比、CD4

/CD8

比值均明显升高 (

0.05). 高剂量EEVLB组Caspase-3蛋白表达上调

Bcl-2蛋白表达下调 (

0.01).

结论:

EEVLB在体内具有良好的抗肿瘤活性

作用可能与荷瘤鼠的凋亡信号通路激活、细胞因子水平升高和免疫功能增强有关.

Abstract

Objective:

To explore the antitumor effects of ethanol extract from

Ventilago leiocarpa

Benth (EEVLB) on sarcoma 180 (S180) tumor-bearing mice and the potential mechanism.

Methods:

Sixty mice were randomly assigned to 6 groups according to a random number table: normal group

model group

5-fluorouracil (5-FU) group (0.02 g•kg

-1

)

and high-

medium-

low-dose EEVLB groups (100

and 56 g of raw material•kg

-1

body weight

respectively)

with 10 mice each group. All treatments were given once daily for 10 consecutive days. Effects of EEVLB on inhibiting tumor growth and immune function in mice were evaluated among all groups after the treatments by detecting tumor inhibition rate

organ index

serum levels of interleukin (IL) -2

-6

-10

CD3

CD4

T lymphocytes

CD4

/CD8

ratio

caspase-3 and Bcl-2.

Results:

EEVLB with different concentrations achieved inhibition of tumor growth

in vivo

wherein the high-dose group showed the most significant reduction in tumor weight and increased apoptosis of tumor cells (

0.05). In addition

both net weight gain and spleen index of mice showed uptrend in EEVLB treatment groups (

0.05). Besides

serum levels of IL-2 and IL-6

percentages of CD3

CD4

T lymphocytes and ratio of CD4

/CD8

in peripheral blood were elevated in high- and medium-dose EEVLB groups compared with the model group (

0.05). Also

upregulation of caspase-3 and downregulation of Bcl-2 were observed at protein levels in the high-dose EEVLB group (

0.01).

Conclusions:

EEVLB exhibits promising antitumor activity

in vivo

. This effect might be due to activation of apoptotic signaling pathway

increase of cytokine levels and enhancement of immune function in tumor-bearing mice.

关键词

Keywords

Ventilago leiocarpa BenthChinese folk medicineYao medicineantitumor effectimmune functioncaspase-3Bcl-2

references

Zhang QY, Wang FX, Jia KK, Kong LD. Natural product interventions for chemotherapy and radiotherapy-induced side effects. Front Pharmacol 2018;9:1253.

Sharifi-Rad J, Ozleyen A, Boyunegmez Tumer T,Oluwaseun Adetunji C, El Omari N, Balahbib A, et al.Natural products and synthetic analogs as a source of antitumor drugs. Biomolecules 2019;9:679.

Demain AL, Vaishnav P. Natural products for cancer chemotherapy. Microb Biotechnol 2011;4:687-699.

Lichota A, Gwozdzinski K. Anticancer activity of natural compounds from plant and marine environment. Int J Molecul Sci 2018;19:3533.

Lin CC, Lin WC, Chang CH, Namba T. Antiinflammatory and hepatoprotective effects of Ventilago leiocarpa.Phytothera Res 1995;9:11-15.

Lin LC, Chou CJ, Kuo YC. Cytotoxic principles from Ventilago leiocarpa. J Natural Product 2001;64:674-676.

Lin CC, Lin JM, Chang CH, Hattori M, Namba T.Pharmacological studies on the crude drug 'Hwang-Jin-Guey' from Taiwan. Phytother Res 1994;8:193-200.

Food and Drug Administration of Guangxi Zhuang Autonomous Region. Quality standards of Yao medicine in Guangxi Zhuang Autonomous Region (Volume Ⅰ). Nanning:Guangxi Science and Technology Press; 2014:98-99.

Yan Z, Lai Z, Lin J. Anticancer properties of traditional Chinese medicine. Combinat Chem High Throughput Screen 2017;20:423-429.

Hu B, Wang SS, Du Q. Traditional Chinese medicine for prevention and treatment of hepatocarcinoma: from bench to bedside. World J Hepatol 2015;7:1209-1232.

Qi F, Li A, Inagaki Y, Gao J, Li J, Kokudo N, et al. Chinese herbal medicines as adjuvant treatment during chemo- or radio-therapy for cancer. BioSci Trends 2010;4:297-307.

Lind EF, Ohashi PS. Mir-155, a central modulator of T-cell responses. Eur J Immunol 2014;44:11-15.

Casati C, Dalerba P, Rivoltini L, Gallino G, Deho P, Rini F, et al. The apoptosis inhibitor protein survivin induces tumor-specific CD8+ and CD4+ T cells in colorectal cancer patients. Cancer Res 2003;63:4507-4515.

Codispoti B, Makeeva I, Sied J, Benincasa C, Scacco S, Tatullo M. Should we reconsider the apoptosis as a strategic player in tissue regeneration? Int J Biol Sci 2019;15:2029-2036.

Harikumar KB, Kuttan G, Kuttan R. Phyllanthus amarus inhibits cell growth and induces apoptosis in Dalton's lymphoma ascites cells through activation of caspase-3 and downregulation of Bcl-2. Integr Cancer Ther 2009;8:190-194.

Jin CY, Moon DO, Choi YH, Lee JD, Kim GY. Bcl-2 and caspase-3 are major regulators in Agaricus blazei-induced human leukemic U937 cell apoptosis through dephoshorylation of Akt. Biol Pharm Bull 2007;30:1432-1437.

Kroemer G, Martin SJ. Caspase-independent cell death.Nature Med 2005;11:725-730.

Wei W, Hao EW, Zhang M, Pan XL, Qin JF, Xie JL, et al.Chemical constituents from Jasminum pentaneurum Hand.-Mazz and their cytotoxicity against human cancer cell lines. Nat Prod Res 2019; 10.1080/14786419.2019.1611810http://doi.org/10.1080/14786419.2019.1611810.

More>

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Polysaccharide of Alocasia cucullata Exerts Antitumor Effect by Regulating Bcl-2, Caspase-3 and ERK1/2 Expressions during Long-Time Administration

In Vitro and In Vivo Evaluation of Antitumor Activity of Ligustrum robustum, A Chinese Herbal Tea

Related Author

No data

Related Institution

School of Traditional Chinese Medicine, Southern Medical University

Clinical and Basic Research Team of TCM Prevention and Treatment of Non-Small Cell Lung Cancer, the Second Clinical College of Guangzhou University of Chinese Medicine

Department of Chinese Medicine, Zhujiang Hospital Affiliated to Southern Medical University

Department of Pharmacy, Jiangmen Central Hospital

West China Hospital/West China School of Nursing, Sichuan University

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Antitumor Effects of Ethanol Extract from Ventilago leiocarpa Benth on Sarcoma 180 Tumor-Bearing Mice and Possible Immune Mechanism*

DOI：10.1007/s11655-021-3440-4

摘要

Abstract

关键词

Keywords

references

Antitumor Effects of Ethanol Extract from Ventilago leiocarpa Benth on Sarcoma 180 Tumor-Bearing Mice and Possible Immune Mechanism^*