Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of <italic style="font-style: italic">Cyathula Officinalis</italic>

Ji-hao MO; Han-kun XIE; Ye-mian ZHOU; Ng Sihan-benjamin; Shao-xia LI; Lei WANG

doi:10.1007/s11655-021-3492-5

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Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis

Original Article | Updated：2021-10-18

- Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis
- Chinese Journal of Integrative Medicine Vol. 27, Issue 10, Pages: 767-773(2021)
- Affiliations：
  
  1.Department of Medical Laboratory, Luoyang Orthopedic Hospital of Henan Province, Orthopedic Institute of Henan Province, Luoyang, Henan Province (471002), China
  2.Miller School of Medicine, University of Miami (33136), Miami, FL,USA
  3.Institute of Technical Biology & Agriculture Engineering,Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei (230031), China
  4.Eatobe Pte. Limited(573969), Singapore
- Author bio：
  
  Prof. WANG Lei, E-mail: lxw561@miami.edu
- Funds：
- DOI：10.1007/s11655-021-3492-5
  CLC：
- Published：01 October 2021，
  
  Published Online：25 August 2021，
  
  Accepted：11 May 2020
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Ji-hao MO, Han-kun XIE, Ye-mian ZHOU, et al. Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis. [J]. Chinese Journal of Integrative Medicine 27(10):767-773(2021)
DOI：

Ji-hao MO, Han-kun XIE, Ye-mian ZHOU, et al. Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis. [J]. Chinese Journal of Integrative Medicine 27(10):767-773(2021) DOI： 10.1007/s11655-021-3492-5.

摘要

Abstract

Objective:

To elucidate the active compounds and the molecular mechanism of

Cyathula Officinalis

as a drug treatment for rheumatoid arthritis (RA).

Methods:

The target genes of active ingredients from

Cyathula Officinalis

were obtained from bioinformatics analysis tool for the molecular mechanism of traditional Chinese medicine. The protein-protein interaction between the target genes were analyzed using STRING and Genemania. The transcriptome of RA patients compared to healthy people (GSE121894) were analyzed using R program package Limma. The relative expression of the target genes was obtained from the RNA-seq datasets. The molecular docking analyses were processed based on the molecular model of estrogen receptor 1 (ESR1) binding with estradiol (PDB ID:1A52). The binding details were analyzed by SYBYL.

Results:

Inokosterone

ecdysterone

and cyaterone were the 3 active ingredients from

Cyathula Officinalis

that bind to target genes. Of all the significantly changed genes from RA patients

ESR1

ADORA1

and ANXA1 were significantly increased in mRNA samples of RA patients.

Conclusion:

ESR1

the transcription factor that binds inokosterone in the molecular binding analysis

is the target protein of

Cyathula Officinalis

关键词

Keywords

rheumatoid arthritisestrogen receptor 1inokosteroneCyathula OfficinalisChinese medicine

references

Aletaha D, Smolen JS. Diagnosis and management of rheumatoid arthritis: a review. JAMA 2018;320:1360-1372.

Bridges SL Jr. The genetics of rheumatoid arthritis:influences on susceptibility, severity, and treatment response. Curr Rheumatol Rep 1999;1:164-171.

Firestein GS. Evolving concepts of rheumatoid arthritis.Nature 2003;423:356-361.

Seca S, Miranda D, Cardoso B, Nogueira HJ, Greten A, et al. Effectiveness of acupuncture on pain, physical function and health-related quality of life in patients with rheumatoid arthritis: a systematic review of quantitative evidence. Chin J Integr Med 2019;25:704-709.

Chandrashekara S. Complementary and alternative medicine in rheumatoid arthritis. Chin J Integr Med 2011;17:731-734.

Guo Q, Wang Y, Xu D, Nossent J, Pavlos NJ, Xu J.Rheumatoid arthritis: pathological mechanisms and modern pharmacologic therapies. Bone Res 2018;6:15.

Kong XY, Wen CP. On research progress of Western and Chinese medicine treatment on pre-rheumatoid arthritis.Chin J Integr Med 2019;25:643-647.

Lopez-Lopez E, Autry RJ, Smith C, Yang W, Paugh SW,Panetta JC, et al. Pharmacogenomics of intracellular methotrexate polyglutamates in patients' leukemia cells in vivo. J Clin Invest 2020;130:6600-6615.

Li Z, Liu J, Hao HQ, Gao YT, Wang Z. Chinese herbal formula Ermiao Powder regulates cholinergic anti-infl ammatory pathway in rats with rheumatoid arthritis. Chin J Integr Med 2020;26:905-912.

Liu Z, Guo F, Wang Y, Li C, Zhang X, Li H, et al. BATMAN-TCM: a bioinformatics analysis tool for molecular mechanism of traditional Chinese medicine. Sci Rep 2016;6:21146.

Szklarczyk D, Franceschini A, Wyder S, Forslund K, Heller D, Huerta-Cepas J, et al. STRING v10: protein-protein interaction networks, integrated over the tree of life. Nucleic Acids Res 2015;43:D447-D452.

Warde-Farley D, Donaldson SL, Comes O, Zuberi K,Badrawi R, Chao P, et al. The GeneMANIA prediction server: biological network integration for gene prioritization and predicting gene function. Nucleic Acids Res 2010;38:W214-W220.

Pettersen EF, Goddard TD, Huang CC, Couch GS,Greenblatt DM, Meng EC, et al. UCSF Chimera—a visualization system for exploratory research and analysis.J Comput Chem 2004;25:1605-1612.

Daina A, Michielin O, Zoete V. Swiss target prediction:updated data and new features for efficient prediction of protein targets of small molecules. Nucleic Acids Res 2019;47:W357-W364.

Huang DW, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protocols 2009;4:44-57.

Gu Z, Eils R, Schlesner M. Complex heatmaps reveal patterns and correlations in multidimensional genomic data.Bioinformatics 2016;32:2847-2849.

Silman AJ, Pearson JE. Epidemiology and genetics of rheumatoid arthritis. Arthritis Res 2002;4(Suppl 3):S265-S272.

Suzuki A, Yamada R, Chang X, Tokuhiro S, Sawada T, Suzuki M, et al. Functional haplotypes of PADI4,encoding citrullinating enzyme peptidylarginine deiminase 4, are associated with rheumatoid arthritis. Nat Genet 2003;34:395-402.

Ometto F, Botsios C, Raffeiner B, Sfriso P, Bernardi L,Todesco S, et al. Methods used to assess remission and low disease activity in rheumatoid arthritis. Autoimm Rev 2010;9:161-164.

Pawlik A, Dziedziejko V, Kurzawski M, Safranow K,Kotrych D, Bohatyrewicz A. Effect of ESR1 and ESR2 gene polymorphisms on rheumatoid arthritis treatment with methotrexate. Pharmacol Rep 2012;64:185-190.

Michio MS, Akio S, Tai M, Ryo N. Pharmacological studies of insect metamorphosing hormone: ponasterone A,ecdysterone, and inokosterone, in the rat. Jap J Pharmacol 1970;20:142-156.

Lang L, Jie Y, Yan Y, Hui Z, Xin H, Chai DK, et al.Phosphorylation of Radix Cyathula officinalis polysaccharide improves its immune-enhancing activity. J Carbohydrate Chem 2020;39:50-62.

Cui HY, Zhang B, An XL. Effect of Chinese herbs for invigorating Kidney on bone morphology of osteoporosis rats. Tianjin Tradit Chin Med (Chin) 1997;35:175-183.

Meng DL, Li X, Huang JJ, Wang Y. Study on the 25-epimers of inokosterone in Achyranthes bidentata Bl.and their antitumor activities. J Shenyang Pharm Univ (Chin)2004;21:266-271.

Toy W, Weir H, Razavi P, Lawson M, Goeppert AU,Mazzola AM, et al. Activating ESR1 mutations differentially affect the efficacy of ER antagonists. Cancer Discov 2017;7:277-287.

Blanchard Z, Vahrenkamp JM, Berrett KC, Arnesen S,Gertz J. Estrogen-independent molecular actions of mutant estrogen receptor 1 in endometrial cancer. Genome Res 2019;29:1429-1441.

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Related Institution

Duke-NUS Medical School

Department of Rheumatology and Immunology, Singapore General Hospital

Medicine Academic Clinical Program, Division of Medicine, Singapore General Hospital

Centre for Quantitative Medicine, Duke-NUS Medical School

College of Basic Medical Sciences, Shanxi University of Chinese Medicine

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Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis

DOI：10.1007/s11655-021-3492-5

摘要

Abstract

关键词

Keywords

references