Attenuation of Oxidative Stress-Induced Cell Apoptosis and Pyroptosis in RSC96 Cells by Salvianolic Acid B

Qian-qian WANG; Meng WANG; Yan LI; Yong-hui LIU; Lian-qing SUN

doi:10.1007/s11655-021-3507-2

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Attenuation of Oxidative Stress-Induced Cell Apoptosis and Pyroptosis in RSC96 Cells by Salvianolic Acid B

Original Article | Updated：2022-03-18

- Attenuation of Oxidative Stress-Induced Cell Apoptosis and Pyroptosis in RSC96 Cells by Salvianolic Acid B
- Chinese Journal of Integrative Medicine Vol. 28, Issue 3, Pages: 243-248(2022)
- Affiliations：
  
  1.Department of Traditional Chinese Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an (710061),China
  2.Department of Peripheral Vascular, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an (710061), China
- Author bio：
  
  Dr. SUN Lian-qing, E-mail: sunlianqing1@126.com
- Funds：
- DOI：10.1007/s11655-021-3507-2
  CLC：
- Published：01 March 2022，
  
  Published Online：27 January 2022，
  
  Accepted：15 December 2020
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Qian-qian WANG, Meng WANG, Yan LI, et al. Attenuation of Oxidative Stress-Induced Cell Apoptosis and Pyroptosis in RSC96 Cells by Salvianolic Acid B. [J]. Chinese Journal of Integrative Medicine 28(3):243-248(2022)
DOI：

Qian-qian WANG, Meng WANG, Yan LI, et al. Attenuation of Oxidative Stress-Induced Cell Apoptosis and Pyroptosis in RSC96 Cells by Salvianolic Acid B. [J]. Chinese Journal of Integrative Medicine 28(3):243-248(2022) DOI： 10.1007/s11655-021-3507-2.

摘要

目的:

研究丹酚酸B是否通过抑制细胞凋亡、焦亡减轻糖尿病周围神经病变损伤.

方法:

RSC96细胞分为对照组 (5.6mmol/L葡萄糖)

高糖组 (125mmol/L葡萄糖) 和丹酚酸B组 (0.1

10μmol/L) . MTT法检测细胞增殖、流式检测细胞活性氧和细胞凋亡率、免疫印迹法检测poly ADP-ribose polymerase、Cleaved-caspase3

Cleaved-caspase9

Bcl-2

Bax

NLRP3

ASC和IL-1β的表达水平.

结果:

相对于对照组

125mmol/L高糖可抑制细胞增殖

促进细胞活性氧的生成

增加细胞凋亡率

上调凋亡、焦亡相关蛋白的表达 (P<0.05) ; 相对于高糖组

丹酚酸B可促进细胞增殖

减少细胞活性氧的生成、下调细胞凋亡率

不仅如此

丹酚酸B可下调凋亡、焦亡相关蛋白的表达 (P<0.05) .

结论:

丹酚酸B可能通过抑制氧化应激诱导的细胞凋亡、焦亡减轻高糖诱导的RSC96细胞损伤.

Abstract

Objective:

To determine whether salvianolic acid B (Sal B) exerts protective effects on diabetic peripheral neuropathy by attenuating apoptosis and pyroptosis.

Methods:

RSC96 cells were primarily cultured with DMEM (5.6 mmol/L glucose)

hyperglycemia (HG

125 mmol/L glucose) and Sal B (0.1

and 10 μmol/L). Cells proliferation was measured by 3-(4

5-cimethylthiazol-2-yl)-2

5-dilphenyltetrazolium bromide assay. Reactive oxygen species (ROS) generation and apoptosis rate were detected by flow cytometry analysis. Western blot was performed to analyze the expressions of poly ADP-ribose polymerase (PARP)

cleaved-caspase 3

cleavedcaspase 9

Bcl-2

Bax

NLRP3

ASC

and interleukin (IL)-1β.

Results:

Treatment with HG at a concentration of 125 mmol/L attenuated cellular proliferation

while Sal B alleviated this injury (

0.05). In addition

Sal B inhibited HG-induced ROS production and apoptosis rate (

0.05). Furthermore

treatment with Sal B down-regulated HG-induced PARP

cleaved-caspase 3

cleaved-caspase 9

Bax

NLRP3

ASC

and IL-1β expression

but mitigated HG-mediated down-regulation of Bcl-2 expression (

0.05).

Conclusion:

Sal B may protect RSC96 cells against HG-induced cellular injury via the inhibition of apoptosis and pyroptosis activated by ROS.

关键词

Keywords

apoptosispyroptosissalvianolic acid Bhyperglycemia

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