Protective Effects of Anthocyanins Extracted from <italic style="font-style: italic">Vaccinium Uliginosum</italic> on 661W Cells Against Microwave-Induced Retinal Damage

Lan YIN; Si-jun FAN; Mao-nian ZHANG

doi:10.1007/s11655-021-3527-y

Your Location：

Home >

Browse articles >

Protective Effects of Anthocyanins Extracted from Vaccinium Uliginosum on 661W Cells Against Microwave-Induced Retinal Damage

Original Article | Updated：2022-06-27

- Protective Effects of Anthocyanins Extracted from Vaccinium Uliginosum on 661W Cells Against Microwave-Induced Retinal Damage
- Chinese Journal of Integrative Medicine Vol. 28, Issue 7, Pages: 620-626(2022)
- Affiliations：
  
  1.Department of Ophthalmology, Chinese PLA General Hospital,Beijing (100853), China
  2.Department of Ophthalmology, PLA Rocket Characteristic Medical Center, Beijing (100853), China
- Author bio：
  
  Prof. ZHANG Mao-nian, E-mail: zmn301@sina.com
- Funds：
- DOI：10.1007/s11655-021-3527-y
  CLC：
- Published：01 July 2022，
  
  Published Online：10 November 2021，
  
  Accepted：16 April 2021
Scan for full text
Lan YIN, Si-jun FAN, Mao-nian ZHANG. Protective Effects of Anthocyanins Extracted from Vaccinium Uliginosum on 661W Cells Against Microwave-Induced Retinal Damage. [J]. Chinese Journal of Integrative Medicine 28(7):620-626(2022)
DOI：

Lan YIN, Si-jun FAN, Mao-nian ZHANG. Protective Effects of Anthocyanins Extracted from Vaccinium Uliginosum on 661W Cells Against Microwave-Induced Retinal Damage. [J]. Chinese Journal of Integrative Medicine 28(7):620-626(2022) DOI： 10.1007/s11655-021-3527-y.

摘要

目的:

研究笃斯越橘提纯花色苷 (VU) 对小鼠视网膜661W细胞微波辐射损伤的保护作用.

方法:

将661W细胞分为6组

包括对照组、模型组[661W细胞微波辐射 (30 mW/cm2

1 h) ]和VU组[661W细胞经VU花青素预处理 (分别为25、50、100和200 µg/mL) 48h后

再经微波辐射30 mW/cm2

1 h]. 采用Heochst染色法检测细胞凋亡指数 (AI) 、丙二醛 (MDA) 和谷胱甘肽 (GSH) 含量

以及超氧化物歧化酶 (SOD) 活性. 实时定量PCR检测Nrf2和HO-1的mRNA表达

Western blot分析检测HO-1蛋白的表达. 分离细胞核和细胞质

进一步检测Nrf2蛋白.

结果:

各组间AI差异显著 (F=322.83

0.05) . 与对照组相比

模型组AI显著升高

4个VU组AI显著降低 (

0.05) . 线性回归分析显示

VU治疗后AI的下降呈剂量依赖性 (r=0.8419

0.05) . VU处理组661W细胞MDA和GSH含量显著低于模型组 (

0.05) . 与模型组相比

VU处理组 (50、100和200 µg/mL) 的SOD活性显著升高 (均

0.05) . Nrf2和HO-1mrna的表达在照射后略有增加

100 μg/mL VU处理组明显增加. 照射后

核内HO-1和Nrf2蛋白的相对表达量略有增加

细胞质变化不明显 (

0.05)

而VU处理组核内和细胞质中的HO-1和Nrf2蛋白的相对表达量均显著增加.

结论:

从VU中提取的花色苷可减少小鼠视网膜光感受器661W细胞的凋亡

稳定细胞膜

减轻氧化损伤

其机制可能是通过激活Nrf2/HO-1信号通路

诱导HO-1转录和翻译.

Abstract

Objective:

To study the protective effect of anthocyanins extracted from

Vaccinium Uliginosum

(VU) on retinal 661W cells against microwave radiation induced retinal injury.

Methods:

661W cells were divided into 6 groups

including control

model [661W cells radiated by microwave (30 mW/cm

1 h)] and VU groups [661W cells pretreated with anthocyanins extracted from VU (25

100 and 200 μg/mL

respectively) for 48 h

and radiated by microwave 30 mW/cm

1 h]. After treatment with different interventions

the cell apoptosis index (AI) was determined using Heochst staining; contents of malonaldehyde (MDA)

glutataione (GSH)

and activity of superoxide dismutase (SOD) were measured. mRNA expressions of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1(HO-1) were detected by real time quantitative polymerase chain reaction

and the expression of HO-1 protein was examined by Western blot analysis. Nucleus and cytoplasm were separated and Nrf2 protein expression was further verified by Western blot analysis.

Results:

There was significant difference in AI among the groups (

=322.83

0.05). Compared with the control group

AI was significantly higher in the model group and was lower in 4 VU-pretreated groups (

0.05). Linear regression analysis showed the decline of AI was in a dose-dependent manner with VU treatment (

=0.8419

0.05). The MDA and GSH contents of 661W cells in VU-treated groups were significantly lower than the model group (

0.05). Compared with the model group

the SOD activity in the VU-treated groups (50

100 and 200 μg/mL) was significantly higher (all

0.05). The Nrf2 and HO-1 mRNA expressions were slightly increased after irradiation

and obviously increased in 100 μg/mL VU-treated group. After irradiation

the relative expressions of HO-1 and Nrf2 proteins in nucleus were slightly increased (

0.05)

and the changes in cytoplasm were not obvious

whereas it was significantly increased in both nucleus and cytoplasm in the VU treatment groups.

Conclusions:

Anthocyanins extracted from VU could reduce apoptosis

stabilize cell membrane

and alleviate oxidant injury of mouse retinal photoreceptor 661W cells. The mechanism might be through activating Nrf2/HO-1 signal pathway and inducing HO-1 transcription and translation.

关键词

Keywords

Vaccinium Uliginosumanthocyaninsmphotoreceptormicrowaveapoptosisnuclear factor erythroid 2-related factor 2/heme oxygenase 1

references

Sanjay S, Stefan D, Anna S, Katharina R, Marloes E,Benjamin S, et al. Radiofrequency electromagnetic field exposure in everyday microenvironments in Europe:a systematic literature review. J Expos Sci Environm Epidemiol 2018;28:147-160.

Matus D, Pavol K, Eva M, Alexandra S, Beata G, Ekaterina N, et al. Microwaves from mobile phone induce reactive oxygen species but not DNA damage, preleukemic fusion genes and apoptosis in hematopoietic stem/progenitor cells. Sci Rep 2019;9:16182-16197.

Stein Y, Udasin IG. Electromagnetic hypersensitivity (EHS,microwave syndrome)—review of mechanisms. Environ Res 2020;186:109445-10955.

Suetov AA, Alekperov SI. Acute ocular lesions after exposure to electromagnetic radiation of ultrahigh frequency(an experimental study). Vestn Oftalmol 2019;135:41-49.

Einat KP, Tamar H, Galia MR, Manuel M, Patricia LB, Niels K, et al. Activation of signaling cascades by weak extremely low frequency electromagnetic fields. Cell Physiol Biochem 2017;43:1533-1546.

Hao YH, Zhang J, Wang H, Wang HY, Dong J, Xu XP, et al.HIF-1α regulates COXⅣ subunits, a potential mechanism of self-protective response to microwave induced mitochondrial damages in neurons. Sci Rep 2018;8:10403-10419.

Jo K, Bae GY, Cho K, Park SS, Suh HJ, Hong KB. An anthocyanin-enriched extract from Vaccinium uliginosum improves signs of skin aging in UVB-induced photodamage.Antioxidants (Basel) 2020;9:844-860.

Kim YH, Bang CY, Won EK, Kim JP, Choung SY.Antioxidant activities of Vaccinium uliginosum L. extract and its active components. J Med Food 2009;12:885-992.

Wang Y, Zhao L, Lu F, Yang X, Deng Q, Ji B, et al. Retinoprotective effects of bilberry anthocyanins via antioxidant, anti-Inflammatory, and anti-apoptotic mechanisms in a visible light-induced retinal degeneration model in pigmented rabbits. Molecules 2015;20:22395-22410.

Ooe E, Kuse Y, Yako T, Sogon T, Nakamura S, Hara H, et al.Bilberry extract and anthocyanins suppress unfolded protein response induced by exposure to blue LED light of cells in photoreceptor cell line. Mol Vis 2018;24:621-632.

Lennart H, Michael C, Tarmo K, Lena H. High radiofrequency radiation at Stockholm Old Town: an explosimeter study including the Royal Castle, Supreme Court, three major squares, and the Swedish Parliament.Mol Clin Oncol 2017;6:462-476.

Wei AM, Yang XS, Wang Y, He GL, Zhou Z, Zhang GB,et al. The injury effects of microwave exposure on visual performance and retinal ganglion cells (RGCs) in rats. Chin J Industr Hygiene Occupat Dis (Chin) 2012;30:172-177.

Brkljača Bottegaro N, Gotić J, Šuran J, Brozić D, Klobu ar K, Bojanić K, et al. Effect of prolonged submaximal exercise on serum oxidative stress biomarkers (d-ROMs, MDA,BAP) and oxidative stress index in endurance horses. BMC Vet Res 2018;14:216-227.

Lee SG, Brownmiller CR, Lee SO, Kang HW. Anti-inflammatory and antioxidant effects of anthocyanins of Trifolium pratense (red clover) in lipopolysaccharide-stimulated RAW-267.4 macrophages. Nutrients 2020;12:1089-1106.

Hernandez DF, Cervantes EL, Luna-Vital DA, Mojica L.Food-derived bioactive compounds with anti-aging potential for nutricosmetic and cosmeceutical products. Crit Rev Food Sci Nutr 2020;8:1-16.

Kutsyr O, Sánchez-Sáez X, Martínez-Gil N, de Juan E, Lax P, Maneu V, et al. Gradual increase in environmental light intensity induces oxidative stress and inflammation and accelerates retinal neurodegeneration. Invest Ophthalmol Vis Sci 2020;61:1-19.

Yin L, Pi YL, Zhang MN. The effect of Vaccinium uliginosum on rabbit retinal structure and light-induced function damage. Chin J Integr Med 2012;18:299-303.

Nutrient Data Laboratory Beltsville Human Nutrition Research Center Agricultural Research Service U.S. Department of Agriculture. USDA database for the proanthocyanidin content of selected foods release 2. 2015[EB/OL]. Available at: http://www.ars.usda.gov/nutrientdata/flavhttp://www.ars.usda.gov/nutrientdata/flav.

Narayanankutty A, Job JT, Narayanankutty V. Glutathione,an antioxidant tripeptide: dual roles in carcinogenesis and chemoprevention. Curr Protein Pept Sci 2019;20:907-917.

Gusti AMT, Qusti SY, Bahijri SM, Toraih EA, Bokhari S,Attallah SM, et al. Glutathione S-transferase (GSTT1 rs17856199) and nitric oxide synthase (NOS2 rs2297518)genotype combination as potential oxidative stress-related molecular markers for type 2 diabetes mellitus. Diabetes Metab Syndr Obes 2021;14:1385-1403.

Farooqui Z, Mohammad RS, Lokhandwala MF, Banday AA.Nrf2 inhibition induces oxidative stress, renal inflammation and hypertension in mice. Clin Exp Hypertens 2020;10:1-6.

Younis NS, Abduldaium MS, Mohamed ME. Protective effect of geraniol on oxidative, inflammatory, and apoptotic alterations in isoproterenol-induced cardiotoxicity: role of the Keap1/Nrf2/HO-1 and PI3K/Akt/mTOR pathways.Antioxidants (Basel) 2020;9:977-987.

Chenet AL, Duarte AR, de Almeida FJS, Andrade CMB,de Oliveira MR. Carvacrol depends on heme oxygenase-1(HO-1) to exert antioxidant, anti-inflammatory, and mitochondria-related protection in the human neuroblastoma SH-SY5Y cells line exposed to hydrogen peroxide.Neurochem Res 2019;44:884-896.

More>

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Effect of Modified Wuzi Yanzong Pill (五子衍宗丸) on Tip60-Mediated Apoptosis in Testis of Male Rats after Microwave Radiation

Fangji Fuling Decoction Alleviates Sepsis by Blocking MAPK14/FOXO3A Signaling Pathway

Asperuloside Promotes Apoptosis of Cervical Cancer Cells through Endoplasmic Reticulum Stress-Mitochondrial Pathway

Naringin Inhibits Colorectal Carcinogenesis by Inhibiting Viability of Colorectal Cancer Cells

Therapeutic Properties of Flavonoids in Treatment of Cancer through Autophagic Modulation:A Systematic Review

Related Author

No data

Related Institution

Department of Traditional Chinese Medicine, Air Force General Hospital

Department of Radiology, Air Force General Hospital

Department of Chinese Medicine, Fangzhuang Community Health Service Center

Department of Andrology, Air Force General Hospital

Department of Critical Care Medicine, Jiangsu Province Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Protective Effects of Anthocyanins Extracted from Vaccinium Uliginosum on 661W Cells Against Microwave-Induced Retinal Damage

DOI：10.1007/s11655-021-3527-y

摘要

Abstract

关键词

Keywords

references