3D Microfluidic System for Evaluating Inhibitory Effect of Chinese Herbal Medicine <italic style="font-style: italic">Oldenlandia diffusa</italic> on Human Malignant Glioma Invasion Combined with Network Pharmacology Analysis

HAN Chun-hui; MA Jing-yun; ZOU Wei; QU Jia-lin; DU Yang; LI Na; LIU Yong; JIN Guo; LENG Ai-jing; LIU Jing

doi:10.1007/s11655-021-3726-1

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3D Microfluidic System for Evaluating Inhibitory Effect of Chinese Herbal Medicine Oldenlandia diffusa on Human Malignant Glioma Invasion Combined with Network Pharmacology Analysis

Original Article | Updated：2023-01-05

- 3D Microfluidic System for Evaluating Inhibitory Effect of Chinese Herbal Medicine Oldenlandia diffusa on Human Malignant Glioma Invasion Combined with Network Pharmacology Analysis
- Chinese Journal of Integrative Medicine Vol. 29, Issue 1, Pages: 52-60(2023)
- Affiliations：
  
  1.Stem Cells Clinical Research Institution, the First Affiliated Hospital of Dalian Medical University, Dalian (116011), Liaoning Province, China
  2.Dalian Innovation Institute of Stem Cell and Precision Medicine, Dalian (116023), Liaoning Province, China
  3.Integrated Chinese and Western Medicine Laboratory, the First Affiliated Hospital of Dalian Medical University, Dalian (116011),Liaoning Province, China
  4.Department of Traditional Chinese Medicine, the First Affiliated Hospital of Dalian Medical University,Dalian (116011), Liaoning Province, China
- Author bio：
  
  Prof. LIU Jing, E-mail: liujing@dmu.edu.cn
- Funds：
- DOI：10.1007/s11655-021-3726-1
  CLC：
- Published：01 January 2023，
  
  Published Online：19 November 2022，
  
  Accepted：23 February 2022
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HAN Chun-hui, MA Jing-yun, ZOU Wei, et al. 3D Microfluidic System for Evaluating Inhibitory Effect of Chinese Herbal Medicine Oldenlandia diffusa on Human Malignant Glioma Invasion Combined with Network Pharmacology Analysis. [J]. Chinese Journal of Integrative Medicine 29(1):52-60(2023)
DOI：

HAN Chun-hui, MA Jing-yun, ZOU Wei, et al. 3D Microfluidic System for Evaluating Inhibitory Effect of Chinese Herbal Medicine Oldenlandia diffusa on Human Malignant Glioma Invasion Combined with Network Pharmacology Analysis. [J]. Chinese Journal of Integrative Medicine 29(1):52-60(2023) DOI： 10.1007/s11655-021-3726-1.

摘要

Abstract

Objective:

To investigate the anti-invasion efficacy of the ethanol extract of

Oldenlandia diffusa

Will. (EEOD) on a three-dimensional (3D) human malignant glioma (MG) cell invasion and perfusion model based on microfluidic chip culture and the possible mechanism of action of

Oldenlandia diffusa

Will. (OD).

Methods:

The comprehensive pharmacodynamic analysis method in this study was based on microfluidic chip 3D cell perfusion culture technology

and the action mechanism of Chinese medicine (CM) on human MG cells was investigated through network pharmacology analysis. First

the components of EEOD were analyzed by ultraperformance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Then

cell viability and apoptosis were assessed to determine the optimum concentration of EEOD for invasion experiments

and two-dimensional (2D) migration and invasion abilities of U87 and U251 MG cells were evaluated using scratch wound and Transwell assays. The possible mechanism underlying the effects of EEOD on glioma was analyzed through a network pharmacology approach.

Results:

Thirty-five compounds of EEOD were detected by UPLC-Q-TOF/MS. EEOD suppressed the viability of MG cells

promoted their apoptosis

and inhibited their migratory and invasive potentials (all

0.05). Network pharmacology analysis showed that OD inhibited the invasion of MG cells by directly regulating MAPK and Wnt pathways through MAPK

EGFR

MYC

GSK3B

and other targets. The anti-invasion effect of OD was also found to be related to the indirect regulation of microtubule cytoskeleton organization.

Conclusion:

EEOD could inhibit the invasion of human MG cells

and the anti-invasion mechanism of OD might be regulating MAPK and Wnt signaling pathways and microtubule cytoskeleton organization.

关键词

Keywords

invasionmalignant gliomaOldenlandia diffusamicrofluidic chipChinese medicine

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The Laboratory for Tumor Molecular Diagnosis, Affiliated People's Hospital of Inner Mongolia Medical University

Department of Gastroenterology, Affiliated People's Hospital of Inner Mongolia Medical University

The Central Laboratory of People' Hospital of Wuhai City

Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, Shaanxi University of Chinese Medicine

State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Science, Xiamen University

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3D Microfluidic System for Evaluating Inhibitory Effect of Chinese Herbal Medicine Oldenlandia diffusa on Human Malignant Glioma Invasion Combined with Network Pharmacology Analysis

DOI：10.1007/s11655-021-3726-1

摘要

Abstract

关键词

Keywords

references