Shexiang Baoxin Pill Regulates Intimal Hyperplasia, Migration, and Apoptosis after Platelet-Derived Growth Factor-BB-Stimulation of Vascular Smooth Muscle Cells via miR-451

Yi-ping LI; Ting-ting QIANG; Ke-yan WANG; Xiao-long WANG

doi:10.1007/s11655-022-2891-6

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Shexiang Baoxin Pill Regulates Intimal Hyperplasia, Migration, and Apoptosis after Platelet-Derived Growth Factor-BB-Stimulation of Vascular Smooth Muscle Cells via miR-451

Original Article | Updated：2022-09-08

- Shexiang Baoxin Pill Regulates Intimal Hyperplasia, Migration, and Apoptosis after Platelet-Derived Growth Factor-BB-Stimulation of Vascular Smooth Muscle Cells via miR-451
- Chinese Journal of Integrative Medicine Vol. 28, Issue 9, Pages: 785-793(2022)
- Affiliations：
  
  Department of Cardiology, Shuguang Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai (201203), China
- Author bio：
  
  Dr. WANG Xiao-long, E-mail: wxlqy0214@163.com
- Funds：
- DOI：10.1007/s11655-022-2891-6
  CLC：
- Published：2022-09，
  
  Published Online：15 July 2022，
  
  Accepted：09 August 2021
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Yi-ping LI, Ting-ting QIANG, Ke-yan WANG, et al. Shexiang Baoxin Pill Regulates Intimal Hyperplasia, Migration, and Apoptosis after Platelet-Derived Growth Factor-BB-Stimulation of Vascular Smooth Muscle Cells via miR-451. [J]. Chinese Journal of Integrative Medicine 28(9):785-793(2022)
DOI：

Yi-ping LI, Ting-ting QIANG, Ke-yan WANG, et al. Shexiang Baoxin Pill Regulates Intimal Hyperplasia, Migration, and Apoptosis after Platelet-Derived Growth Factor-BB-Stimulation of Vascular Smooth Muscle Cells via miR-451. [J]. Chinese Journal of Integrative Medicine 28(9):785-793(2022) DOI： 10.1007/s11655-022-2891-6.

摘要

目的:

总结中医药防治新型冠状病毒肺炎 (以下简称“新冠肺炎”) 诊疗方案的用药规律

为临床诊治提供参考.

方法:

搜索从2019年12月8日到2020年3月10日的各省市政府网站和官方媒体网站上的中医药防治新冠肺炎诊疗方案. 分防治阶段和地区对诊疗方案的中药组方进行用药频次、中药配伍、聚类以及复杂网络分析等.

结果:

在27个中医诊治方案中

治疗处方共有203个

用药频次较高的前4位中药分别为: 甘草、杏仁、麻黄、藿香

核心组合是麻黄和杏仁. 在48个预防处方中

使用频率最高的中药分别为: 黄芪、甘草、防风、金银花等. 核心配伍处方为黄芪、甘草、防风

是玉平风散的主要成分. 中国华东地区的诊疗方案中

防治处方共45个

使用频率最多的中药是甘草、藿香、陈皮和石膏等. 中国华北地区的诊疗方案中

防治处方共50个

使用频率最多的中药是藿香、杏仁、麻黄和茯苓等.

结论:

在预防新冠肺炎方面

中医药主要侧重于提高人体免疫力; 治疗上

则重在清肺利湿. 中药处方的应用因地制宜

可能是由于气候和环境的差异

这些结果有助于临床医生快速制定中医药诊疗计划.

Abstract

Objective:

To investigate the regulatory roles of Shexiang Baoxin Pill (SXBXW) in neointimal formation and vascular smooth muscle cells (VSMCs) invasion and apoptosis as well as the potential molecular mechanisms using cultured VSMCs model of vascular injury (platelet-derived growth factor (PDGF)-BB-timulated)

in vitro

Methods:

VSMCs were randomly assigned to 5 groups: blank

PDGF-BB (20 ng/mL+ 0.1% DMSO)

SXBXW-L (PDGF-BB 20 ng/mL + SXBXW low dose 0.625 g/L)

SXBXW-M (PDGF-BB 20 ng/mL + SXBXW medium dose 1.25 g/L) and SXBXW-H (PDGF-BB 20 ng/mL+ SXBXW high dose 2.5 g/L) group. Cell proliferation was assessed using cell counting kit-8 (CCK-8) assay and bromodeoxyuridine (BrdU) incorporation assay

the migration effects were detected by Transwell assay

cell apoptosis rate was measured by the Annexin V/propidium iodide (PI) apoptosis kit. The markers of contractile phenotype of VSMCs were detected with immunofluorescent staining. To validate the effects of miR-451 in regulating proliferation

migration and apoptosis treated with SXBXW

miR-451 overexpression experiments were performed

the VSMCs were exposed to PDGF-BB 20 ng/mL + 0.1% DMSO and later divided into 4 groups: mimic-NC (multiplicity of infection

MOI=50)

SXBXW (1.25 g/L) + mimic-NC

mimic-miR451 (MOI=50)

and SXBXW (1.25 g/L) + mimic-miR451

and alterations of proteins related to the miR-451 pathway were analyzed using Western blot.

Results:

PDGF-BB induced VSMCs injury causes acceleration of proliferation and migration. SXBXW inhibited phenotypic switching

proliferation and migration and promoted cell apoptosis in PDGF-BB-induced VSMCs. In addition

miR-451 was shown to be down-regulated in the VSMCs following PDGF-BB stimulation. SXBXW treatment enhanced the expression of miR-451 in PDGF-BB-induced VSMCs (

0.05). Compared with SXBXW + mimic-NC and mimic-miR451 groups

the expression of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (Ywhaz) and p53 was further reduced in SXBXW + mimic-miR451 group

while activating transcription factor 2 (ATF2) was increased in VSMCs (

0.05).

Conclusion:

SXBXW regulated proliferation

migration and apoptosis via activation of miR-451 through ATF2

p53 and Ywhaz in PDGF-BB-stimulated VSMCs.

关键词

麝香保心丸miR-451血小板源性生长因子BB刺激的血管平滑肌细胞增殖凋亡

Keywords

Shexiang Baoxin PillsmiR-451platelet-derived growth factor-BB-stimulated vascular smooth muscle cells proliferationapoptosis

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