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Composite Sophora Colon-Soluble Capsule Ameliorates DSS-Induced Ulcerative Colitis in Mice via Gut Microbiota-Derived Butyric Acid and NCR+ ILC3
- Chinese Journal of Integrative Medicine Vol. 29, Issue 5, Pages: 424-433(2023)
- Affiliations：
1.Medical School of Chinese People's Liberation Army, Beijing(100853), China
2.Department of Traditional Chinese Medicine, The Second Medical Center & National Clinical Research Center of Geriatric Diseases, Chinese People's Liberation Army General Hospital, Beijing (100853), China
3.Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing (100850), China
- Author bio：
Prof. TONG Zhan-qi, E-mail: tong.zhanqi@163.com
- Funds：
DOI：10.1007/s11655-022-3317-1
CLC：Published：2023-05，
Published Online：12 April 2022，
Accepted：28 May 2021
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CHEN Ming-jun, FENG Yang, GAO Lu, et al. Composite Sophora Colon-Soluble Capsule Ameliorates DSS-Induced Ulcerative Colitis in Mice via Gut Microbiota-Derived Butyric Acid and NCR+ ILC3. [J]. Chinese Journal of Integrative Medicine 29(5):424-433(2023)
CHEN Ming-jun, FENG Yang, GAO Lu, et al. Composite Sophora Colon-Soluble Capsule Ameliorates DSS-Induced Ulcerative Colitis in Mice via Gut Microbiota-Derived Butyric Acid and NCR+ ILC3. [J]. Chinese Journal of Integrative Medicine 29(5):424-433(2023) DOI： 10.1007/s11655-022-3317-1.
摘要
Abstract
Objective:
2
To investigate the effects of composite Sophora colon-soluble Capsule (CSCC) on gut microbiota-mediated short-chain fatty acids (SCFAs) production and downstream group 3 innate lymphoid cells (ILC3s) of dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
Methods:
2
The main components of CSCC were analyzed by hybrid ultra-high-performance liquid chromatography ion mobility spectromety quadrupole time-of-flight mass spectrometry (UHPLC-IM-QTOF/MS). Twenty-four male BALB/c mice were randomly divided into 4 groups (
n
=6) by using a computer algorithm-generated random digital
including control
DSS model
mesalazine
and CSCC groups. A DSS-induced colitis mice model was established to determine the effects of CSCC by recording colonic weight
colonic length
index of colonic weight
and histological colonic score. The variations in ILC3s were assessed by immunofluorescence and flow cytometry. The results of gut microbiota and SCFAs were acquired by 16s rDNA and gas chromatography-mass spectrometry (GC-MS) analysis. The expression levels of NCR
+
ILC3
-
CCR6
+
Nkp46
-
(Lti) ILC3
-
and ILCreg-specific markers were detected by enzyme-linked immunosorbent assay
and real-time quantitative polymerase chain reaction and Western blot
respectively.
Results:
2
The main components of CSCC were matrine
ammothamnine
Sophora flavescens
neoalcohol J
and
Sophora oxytol
U. After 7 days of treatment
CSCC significantly alleviated colitis by promoting the reproduction of intestinal probiotics manifested as upregulation of the abundance of
Bacteroidetes
species and specifically the
Bacteroidales
_S24-7 genus (
P
<
0.05). Among the SCFAs
the content of butyric acid increased the most after CSCC treatment. Meanwhile
compared with the model group
Lti ILC3s and its biomarkers were significantly downregulated and NCR
+
ILC3s were significantly elevated in the CSCC group (
P
<
0.01). Further experiments revealed that ILC3s were differentiated from Lti ILC3s to NCR
+
ILC3s
resulting in interleukin-22 production which regulates gut epithelial barrier function.
Conclusion:
2
CSCC may exert a therapeutic effect on UC by improving the gut microbiota
promoting metabolite butyric acid production
and managing the ratio between NCR
+
ILC3s and Lti ILC3s.
关键词
Keywords
composite Sophora colon-soluble capsuleulcerative colitisgroup 3 innate lymphoid cellsgut microbiotabutyric acid
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