Andrographolide Inhibits Proliferation and Promotes Apoptosis in Bladder Cancer Cells by Interfering with NF-κB and PI3K/AKT Signaling <italic style="font-style: italic">In Vitro</italic> and <italic style="font-style: italic">In Vivo</italic>

Lei XUAN; Jing-hai HU; Ran BI; Si-qi LIU; Chun-xi WANG

doi:10.1007/s11655-022-3464-4

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Andrographolide Inhibits Proliferation and Promotes Apoptosis in Bladder Cancer Cells by Interfering with NF-κB and PI3K/AKT Signaling In Vitro and In Vivo

Original Article | Updated：2022-03-29

- Andrographolide Inhibits Proliferation and Promotes Apoptosis in Bladder Cancer Cells by Interfering with NF-κB and PI3K/AKT Signaling In Vitro and In Vivo
- Chinese Journal of Integrative Medicine Vol. 28, Issue 4, Pages: 349-356(2022)
- Affiliations：
  
  Department of Urology, the First Hospital of Jilin University,Changchun (130021), China
- Author bio：
  
  Prof. WANG Chun-xi, E-mail: chunxi@jlu.edu.cn
- Funds：
- DOI：10.1007/s11655-022-3464-4
  CLC：
- Published：2022-04，
  
  Published Online：19 January 2022，
  
  Accepted：11 June 2021
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Lei XUAN, Jing-hai HU, Ran BI, et al. Andrographolide Inhibits Proliferation and Promotes Apoptosis in Bladder Cancer Cells by Interfering with NF-κB and PI3K/AKT Signaling In Vitro and In Vivo. [J]. Chinese Journal of Integrative Medicine 28(4):349-356(2022)
DOI：

Lei XUAN, Jing-hai HU, Ran BI, et al. Andrographolide Inhibits Proliferation and Promotes Apoptosis in Bladder Cancer Cells by Interfering with NF-κB and PI3K/AKT Signaling In Vitro and In Vivo. [J]. Chinese Journal of Integrative Medicine 28(4):349-356(2022) DOI： 10.1007/s11655-022-3464-4.

摘要

目的:

探索穿心莲内酯对膀胱癌细胞和荷人膀胱癌原位移植瘤小鼠模型的影响.

方法:

在体外实验中

用0-40 μmol/L穿心莲内酯处理T24细胞

用0-80 μmol/L穿心莲内酯处理5637细胞. 使用CCK-8、成克隆实验、划痕实验和transwell测定法评估膀胱癌细胞的生长、迁移和浸润. 使用流式细胞术测定膀胱癌细胞凋亡情况. 在体内研究中

通过5637膀胱癌细胞系构建荷人膀胱癌原位移植瘤小鼠模型评估了穿心莲内酯(10 mg/kg)的抗肿瘤作用

并通过免疫印迹法测定了NF-κB和PI3K/AKT相关蛋白的水平.

结果:

穿心莲内酯抑制膀胱癌细胞的生长、迁移和浸润 (

0.05 或

0.01) . 穿心莲内酯在膀胱癌细胞系中诱导了内在的线粒体依赖性细胞凋亡. 另一方面

穿心莲内酯抑制小鼠体内膀胱癌细胞的生长 (

0.01) . 在体外和体内实验中

穿心莲内酯均抑制了p65、p-AKT的表达 (

0.05或

0.01) .

Abstract

Objective:

To explore the inﬂuences of andrographolide (Andro) on bladder cancer cell lines and a tumor xenograft mouse model bearing 5637 cells.

Methods:

For

in vitro

experiments

T24 cells were stimulated with Andro (0–40 μmol/L) and 5637 cells were stimulated with Andro (0 to 80 μmol/L). Cell growth

migration

and infiltration were assessed using cell counting kit-8

colony formation

wound healing

and transwell assays. Apoptosis rate was examined using ﬂow cytometry. In

in vivo

study

the antitumor effect of Andro (10 mg/kg) was evaluated by 5637 tumor-bearing mice

and levels of nuclear factor κB (NF-κB) and phosphoinositide 3-kinase/AKT related-proteins were determined by immunoblotting.

Results:

Andro suppressed growth

migration

and infiltraion of bladder cancer cells (

≤0.05 or

≤ 0.01). Additionally

Andro induced intrinsic mitochondria-dependent apoptosis in bladder cancer cell lines. Furthermore

Andro inhibited bladder cancer growth in mice (

≤0.01). The expression of p65

p-AKT were suppressed by Andro treatment

in vitro

and

in vivo

(

≤ 0.05 or

≤ 0.01).

Conclusions:

Andrographolide inhibits proliferation and promotes apoptosis in bladder cancer cells by interfering with NF-κB and PI3K/AKT signaling

in vitro

and

in vivo

关键词

Keywords

andrographolideChinese medicinebladder cancernuclear factor κBPI3K-AKT

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Department of Pharmacology, Southwest Medical University

Sichuan Institute for Translational Chinese Medicine, Translational Chinese Medicine Key Laboratory of Sichuan Province, Sichuan Academy of Chinese Medicine Sciences

Hunan Provincial Key Laboratory of the Research and Development of Novel Pharmaceutical Preparations, College of Pharmacy, Changsha Medical University

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Andrographolide Inhibits Proliferation and Promotes Apoptosis in Bladder Cancer Cells by Interfering with NF-κB and PI3K/AKT Signaling In Vitro and In Vivo

DOI：10.1007/s11655-022-3464-4

摘要

Abstract

关键词

Keywords

references