Molecular Mechanism of Electroacupuncture Regulating Cerebral Arterial Contractile Protein in Rats with Cerebral Infarction Based on MLCK Pathway

LI Jing; ZHANG Min; HE Ying; DU Yuan-hao; ZHANG Xue-zhu; Rainer Georgi; Bernhard Kolberg; XU Yan-long

doi:10.1007/s11655-022-3468-0

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Molecular Mechanism of Electroacupuncture Regulating Cerebral Arterial Contractile Protein in Rats with Cerebral Infarction Based on MLCK Pathway

Acupuncture Research | Updated：2023-01-05

- Molecular Mechanism of Electroacupuncture Regulating Cerebral Arterial Contractile Protein in Rats with Cerebral Infarction Based on MLCK Pathway
- Chinese Journal of Integrative Medicine Vol. 29, Issue 1, Pages: 61-68(2023)
- Affiliations：
  
  1.Institute of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin (300193), China
  2.Max Planck Institute for Medical Research, Heidelberg(69120), Germany
  3.Uniklinik Mannheim, Mannheim (68167),Germany
  4.Department of Acupuncture and Moxibustion,Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou (730050), China
- Author bio：
  
  Prof. DU Yuan-hao, E-mail: ddyh64@tjutcm.edu.cn
- Funds：
- DOI：10.1007/s11655-022-3468-0
  CLC：
- Published：01 January 2023，
  
  Published Online：27 March 2022，
  
  Accepted：08 June 2021
Scan for full text
LI Jing, ZHANG Min, HE Ying, et al. Molecular Mechanism of Electroacupuncture Regulating Cerebral Arterial Contractile Protein in Rats with Cerebral Infarction Based on MLCK Pathway. [J]. Chinese Journal of Integrative Medicine 29(1):61-68(2023)
DOI：

LI Jing, ZHANG Min, HE Ying, et al. Molecular Mechanism of Electroacupuncture Regulating Cerebral Arterial Contractile Protein in Rats with Cerebral Infarction Based on MLCK Pathway. [J]. Chinese Journal of Integrative Medicine 29(1):61-68(2023) DOI： 10.1007/s11655-022-3468-0.

摘要

Abstract

Objective:

To explore the effect of electroacupuncture (EA) intervention on the vasoconstriction of cerebral artery smooth muscle cells after cerebral infarction.

Methods:

Male Wistar rats were randomly divided into 3 groups by a random number table: the model group (

=24)

the EA group (

=24)

and the normal group (

=6). The model and the EA groups were divided into different time subgroups at 0.5

and 6 h after middle cerebral artery occlusion (MCAO)

with 6 rats in each subgroup. MCAO model was established using intraluminal suture occlusion method. The EA group was given EA treatment at acupoint Shuigou (GV 26) instantly after MCAO for 20 min. The contents of cerebrovascular smooth muscle MLCK

the 3 subunits of myosin light chain phosphatase (MLCP) MYPT1

PP1c-δ and M20

as well as myosin-ATPase activity were detected using immunohistochemistry and Western blotting.

Results:

The overall expression level of the MYPT1 and PP1c-δ in the model group was significantly higher (

0.01). After EA intervention

the 0.5 h group expression level was close to that of the normal group (

0.05)

and the other subgroups were still significantly higher than the normal group (

0.01). After EA intervention

the expression level of each subgroup was significantly lower than the corresponding model group. There was a significant difference between the 0.5 and 1 h subgroups (

0.01)

while a difference was also observed between the 3 and 6 h subgroups (

0.05). The dynamic change rule gradually increased with the prolongation of infarction time within 6 h after infarction.

Conclusion:

EA intervention can inhibit contraction of cerebral vascular smooth muscle cells and regulate smooth muscle relaxation by regulating MLCK pathway.

关键词

Keywords

infarctionvasomotorMLCK pathwayShuigou (GV 26)electroacupuncture

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Institute of Acupuncture and Moxibustion, Shaanxi University of Chinese Medicine

Department of Preventive Treatment of Disease, Binhai New Area Hospital of Traditional Chinese Medicine, Fourth Teaching Hospital of Tianjin University of Traditional Chinese Medicine

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Department of Internal Medicine, Mannheim Medical School of Heidelberg University

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