Baicalein Attenuates Severe Polymicrobial Sepsis via Alleviating Immune Dysfunction of T Lymphocytes and Inflammation

Hai-yan CHEN; Shu ZHANG; Jun LI; Na HUANG; Jin SUN; Bao-hua LI; Jun YANG; Zong-fang LI

doi:10.1007/s11655-022-3510-7

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Baicalein Attenuates Severe Polymicrobial Sepsis via Alleviating Immune Dysfunction of T Lymphocytes and Inflammation

Original Article | Updated：2022-07-20

- Baicalein Attenuates Severe Polymicrobial Sepsis via Alleviating Immune Dysfunction of T Lymphocytes and Inflammation
- Chinese Journal of Integrative Medicine Vol. 28, Issue 8, Pages: 711-718(2022)
- Affiliations：
  
  1.National & Local Joint Engineering Research Center of Biodiagnosis and Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an (710004), China
  2.Core Research Laboratory, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an (710004), China
  3.Department of General Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an (710004), China
  4.Shaanxi Provincial Clinical Research Center for Hepatic & Splenic Diseases, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an (710004),China
  5.Department of Pathology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an (710004), China
- Author bio：
  
  Prof. LI Zong-fang, E-mail: lzf2568@gmail.com
- Funds：
- DOI：10.1007/s11655-022-3510-7
  CLC：
- Published：2022-08，
  
  Published Online：30 March 2022，
  
  Accepted：15 June 2021
Scan for full text
Hai-yan CHEN, Shu ZHANG, Jun LI, et al. Baicalein Attenuates Severe Polymicrobial Sepsis via Alleviating Immune Dysfunction of T Lymphocytes and Inflammation. [J]. Chinese Journal of Integrative Medicine 28(8):711-718(2022)
DOI：

Hai-yan CHEN, Shu ZHANG, Jun LI, et al. Baicalein Attenuates Severe Polymicrobial Sepsis via Alleviating Immune Dysfunction of T Lymphocytes and Inflammation. [J]. Chinese Journal of Integrative Medicine 28(8):711-718(2022) DOI： 10.1007/s11655-022-3510-7.

摘要

目的:

探讨黄芩素对脓毒症所诱发的免疫功能障碍和器官损伤的作用研究.

方法:

采用盲肠结扎穿刺法(CLP)建立SD大鼠脓毒症模型. 将无特异致病菌的大鼠随机分为假手术组、CLP组和CLP +黄芩素组 (

= 16/组). CLP + Bai组的大鼠在CLP造模后的1小时和10小时分别静脉注射黄芩素(20 mg/kg). 评估各组大鼠的生存率、细菌载量和器官损伤. 然后在6、12、24 h

对每组进行评估并观察黄芩素对脓毒症大鼠的免疫细胞和炎性细胞因子的影响.

结果:

黄芩素治疗能显著提高脓毒症大鼠的存活率

降低细菌载量

通过苏木精和伊红染色观察到可以减轻组织损伤(脾脏、肝脏和肺). 黄芩素处理后脓毒症大鼠的外周血和脾脏中发现

CD3

CD4

T细胞比例及CD4

/CD8

比值显著升高 (均

0.05). 而且

黄芩素能降低造模后24 h的脓毒症大鼠的白细胞和脾脏细胞凋亡率(

0.05). 黄芩素还能显著降低脾脏中肿瘤坏死因子α和白细胞介素-6 (IL-6)的水平

升高IL-10的水平

并能显著提高galectin - 9的表达水平(

0.01).

结论:

黄芩素可能是一种有效的免疫调节剂

可减轻严重腹腔性脓毒症的压倒性炎症反应.

Abstract

Objective:

To investigate the effect of baicalein on polymicrobial sepsis-induced immune dysfunction and organ injury.

Methods:

A sepsis model was induced in Sprague-Dawley rats via caecal ligation and puncture (CLP). Specific pathogen free rats were randomly divided into a sham group

CLP group and CLP + baicalein (Bai) group (

=16 each). Rats in the CLP + Bai group were intravenously injected with baicalein (20 mg/kg) at 1 and 10 h after CLP. Survival rate

bacterial load

and organ damage were assessed. Then each group was evaluated at 6

and 24 h to investigate the effect of baicalein on immune cells and inflammatory cytokines in septic rats.

Results:

Baicalein treatment significantly improved the survival of septic rats

decreased the bacterial burden

and moderated tissue damage (spleen

liver

and lung)

as observed by haematoxylin and eosin staining. Septic rats treated with baicalein had strikingly increased proportions of CD3

CD4

T cells and ratios of CD4

/CD8

T cells in the peripheral blood and spleen (all

0.05). Moreover

baicalein treatment decreased the apoptotic rate of whole white blood cells and spleen cells at 24 h after surgery (

0.05). Baicalein significantly reduced the levels of tumor necrosis factor α and interleukin-6 (IL-6) and increased IL-10

and the expression levels of galectin 9 were also raised in the spleen (

0.01).

Conclusion:

Baicalein may be an effective immunomodulator that attenuates overwhelming inflammatory responses in severe abdominal sepsis.

关键词

黄芩素中药脓毒症脾脏炎症淋巴细胞

Keywords

baicaleinChinese medicinesepsisspleeninflammationlymphocytes

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