Protective Effect and Mechanism of Kuntai Capsule on Angiotensin Ⅱ-Induced Hypertension in Ovariectomized Rats

GE Xiao-fen; LI Sha-sha; LIU Yan-hua; LU Mei-qiu; SU Hui-na; YANG Xin; SUN Xiao-wan

doi:10.1007/s11655-022-3514-3

Your Location：

Home >

Browse articles >

Protective Effect and Mechanism of Kuntai Capsule on Angiotensin Ⅱ-Induced Hypertension in Ovariectomized Rats

Original Article | Updated：2023-05-25

- Protective Effect and Mechanism of Kuntai Capsule on Angiotensin Ⅱ-Induced Hypertension in Ovariectomized Rats
- Chinese Journal of Integrative Medicine Vol. 29, Issue 6, Pages: 526-533(2023)
- Affiliations：
  
  1.Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing (100044), China
  2.Department of Obstetrics and Gynecology, Dongying People's Hospital, Dongying, Shandong Province (257000), China
- Author bio：
  
  Prof. YANG Xin, E-mail: xinyang_2003@sina.com
- Funds：
- DOI：10.1007/s11655-022-3514-3
  CLC：
- Published：2023-06，
  
  Published Online：03 November 2022，
  
  Accepted：13 July 2020
Scan for full text
GE Xiao-fen, LI Sha-sha, LIU Yan-hua, et al. Protective Effect and Mechanism of Kuntai Capsule on Angiotensin Ⅱ-Induced Hypertension in Ovariectomized Rats. [J]. Chinese Journal of Integrative Medicine 29(6):526-533(2023)
DOI：

GE Xiao-fen, LI Sha-sha, LIU Yan-hua, et al. Protective Effect and Mechanism of Kuntai Capsule on Angiotensin Ⅱ-Induced Hypertension in Ovariectomized Rats. [J]. Chinese Journal of Integrative Medicine 29(6):526-533(2023) DOI： 10.1007/s11655-022-3514-3.

摘要

Abstract

Objective:

To explore the protective effect and mechanism of Kuntai (KT) Capsule on angiotensin Ⅱ (AngⅡ)-induced hypertension in ovariectomized (OVX) rats.

Methods:

Fifty-four rats were randomly divided into 6 groups according to a random number table

9 in each group: control

OVX sham+AngⅡ

OVX

OVX+AngⅡ

OVX+AngⅡ+E

and OVX+AngⅡ+KT. OVX rats model was constructed by retroperitoneal bilateral ovariectomy. After 4 weeks of pretreatment with KT Capsule [0.8 g/(kg•d) and 17-β-estradiol (E

1.2 mg/(kg•d)] respectively

AngⅡ was injected into a micro-osmotic pump with a syringe to establish a hypertensive rat model. Blood pressure of rat tail artery was measured in a wake state of rats using a non-invasive sphygmomanometer. Blood pressure changes were compared between the intervention groups (OVX+AngⅡ+KT

OVX+AngⅡ+E

) and the negative control group (OVX+AngⅡ). Serum malondialdehyde (MDA) level and superoxide dismutase (SOD) activity were detected respectively. The expressions of oxidative stress-related protein superoxide dismutase2 (SOD2) and anti-thioredoxin (TRX)

autophagy marker protein [beclin1

light chain (LC) 3Ⅱ/Ⅰ ratio and autophagy canonical pathway protein phosphatidylinositol 3-kinase (PI3K)/serine/threonine kinase (AKT)-mammalian target of rapamycin (mTOR)] were evaluated by Western blotting.

Results:

Compared with the OVX+AngⅡ group

the systolic blood pressure of OVX+AngⅡ+KT group was significantly lowered (

0.05) but not the diastolic blood pressure. Besides

SOD2 and TRX protein levels in mycardial tissues were significantly reduced in the OVX+AngⅡ+KT group compared with the OVX+AngⅡ group (

0.05). Oxidative stress serum markers MDA and SOD were down- and up-regulated in the OVX+AngⅡ+KT group

respectively (

0.05). Compared with OVX+AngⅡ group

the levels of cardiac proteins beclin-1 and LC3Ⅱ/LC3Ⅰ in OVX+AngⅡ+KT group were also up-regulated (

0.05)

and the expression levels of p-PI3K

p-AKT and mTOR protein were down-regulated (

0.05).

Conclusion:

KT could protect blood pressure of AngⅡ-induced OVX rats by inhibiting oxidative stress and up-regulating protective autophagy.

关键词

Keywords

Kuntai CapsuleChinese medicineperimenopausal hypertensionangiotensin Ⅱ

references

Matthews KA, Crawford SL, Chae CU, Everson-Rose SA, Sowers MF, Sternfeld B, et al. Are changes in cardiovascular disease risk factors in midlife women due to chronological aging or to the menopausal transition? J Am Coll Cardiol 2009; 54:2366-2373.

Kannel WB. Metabolic risk factors for coronary heart disease in women: perspective from the Framingham Study. Am Heart J 1987; 114:413-419.

Kearney PM, Whelton M, Reynolds K, Muntner P, Whelton PK, He J. Global burden of hypertension: analysis of worldwide data. Lancet 2005; 365:217-223.

Ramezani Tehrani F, Bahri M, Gholami R, Hashemi S, Nakhoda K, Azizi F. Secular trend of menopausal age and related factors among Tehrani women born from 1930 to 1960: Tehran Lipid and Glucose Study. Arch Iran Med 2014; 17:406-410.

Lim HS, Kim TH, Lee HH, Park YH, Kim JM, Lee BR. Hypertension and age at onset of natural menopause in Korean postmenopausal women: results from the Korea National Health and Nutrition Examination Survey (2008–2013). Maturitas 2016; 90:17-23.

Yanes LL, Romero DG, Iliescu R, Zhang H, Davis D, Reckelhoff JF. Postmenopausal hypertension: role of the renin-angiotensin system. Hypertension 2010; 56:359-363.

Dobarro D, Gómez-Rubín MC, Sanchez-Recalde A, Moreno R, Galeote G, Jimenez-Valero S, et al. Current pharmacological approach to restore endothelial dysfunction. Cardiovasc Hematol Agents Med Chem 2009; 7:212-222.

Langrish JP, Mills NL, Bath LE, Warner P, Webb DJ, Kelnar CJ, et al. Cardiovascular effects of physiological and standard sex steroid replacement regimens in premature ovarian failure. Hypertension 2009; 53:805-811.

Mercuro G, Zoncu S, Saiu F, Mascia M, Melis GB, Rosano GM. Menopause induced by oophorectomy reveals a role of ovarian estrogen on the maintenance of pressure homeostasis. Maturitas 2004; 47:131-138.

Pu D, Tan R, Yu Q, Wu J. Metabolic syndrome in menopause and associated factors: a meta-analysis. Climacteric 2017; 20:583-591.

Fung MM, Poddar S, Bettencourt R, Jassal SK, Barrett-Connor E. A cross-sectional and 10-year prospective study of postmenopausal estrogen therapy and blood pressure, renal function, and albuminuria: the Rancho Bernardo Study. Menopause 2011; 18:629-637.

Sun AJ, Wang YP, Gu B, Zheng TP, Lin SQ, Bai WP, et al. A multi-center, randomized, controlled and open clinical trial of Heyan Kuntai Capsule and hormone therapy in perimenopausal women. Chin J Integr Med (Chin) 2018; 24:487-493.

Zhang JJ, Fang L, Shi LY, Lai ZW, Lu ZY, Xiong JQ, et al. Protective effects and mechanisms investigation of Kuntai Capsule on the ovarian function of a novel model with accelerated aging ovaries. J Ethnopharmacol 2017; 195:173-181.

Pollow DP, Romero-Aleshire MJ, Sanchez JN, Konhilas JP, Brooks HL. AngⅡ-induced hypertension in the VCD mouse model of menopause is prevented by estrogen replacement during perimenopause. Am J Physiol Regulat Integr Comparat Physiol 2015; 309: R1546-R1552.

Benjamin EJ, Virani SS, Callaway CW, Chamberlain AM, Chang AR, Cheng S, et al. Heart disease and stroke statistics—2018 update: a report from the American Heart Association. Circulation 2018; 137: e467-e492.

Coylewright M, Reckelhoff JF, Ouyang P. Menopause and hypertension: an age-old debate. Hypertension 2008; 51:952-959.

Matrai M, Hetthéssy JR, Nadasy GL, Szekacs B, Mericli M, Acs N, et al. Estrogen therapy may counterbalance eutrophic remodeling of coronary arteries and increase bradykinin relaxation in a rat model of menopausal hypertension. Menopause 2016; 23:778-783.

Huang Z, Han Z, Ye B, Dai Z, Shan P, Lu Z, et al. Berberine alleviates cardiac ischemia/reperfusion injury by inhibiting excessive autophagy in cardiomyocytes. Eur J Pharmacol 2015; 762:1-10.

Yu L, Li Q, Yu B, Yang Y, Jin Z, Duan W, et al. Berberine attenuates myocardial ischemia/reperfusion injury by reducing oxidative stress and inflammation response: role of silent information regulator 1. Oxid Med Cell Longev 2016; 2016:1689602.

Gasiorowski K, Lamer-Zarawska E, Leszek J, Parvathaneni K, Yendluri BB, Blach-Olszewska Z, et al. Flavones from root of Scutellaria baicalensis Georgi: drugs of the future in neurodegeneration? CNS Neurol Disord Drug Targets 2011; 10:184-191.

Guzik TJ, Touyz RM. Oxidative stress, inflammation, and vascular aging in hypertension. Hypertension 2017; 70:660-667.

Dinh QN, Drummond GR, Sobey CG, Chrissobolis S. Roles of inflammation, oxidative stress, and vascular dysfunction in hypertension. Biomed Res Int 2014; 2014:406960.

Egler RA, Fernandes E, Rothermund K, Sereika S, de Souza-Pinto N, Jaruga P, et al. Regulation of reactive oxygen species, DNA damage, and c-Myc function by peroxiredoxin 1. Oncogene 2005; 24:8038-8050.

Wei X, Zhu X, Hu N, Zhang X, Sun T, Xu J, et al. Baicalin attenuates angiotensin Ⅱ-induced endothelial dysfunction. Biochem Biophys Res Commun 2015; 465:101-107.

Androulakis ES, Tousoulis D, Papageorgiou N, Tsioufis C, Kallikazaros I, Stefanadis C. Essential hypertension: is there a role for inflammatory mechanisms? Cardiol Rev 2009; 17:216-221.

Ren J, Taegtmeyer H. Too much or not enough of a good thing—the Janus faces of autophagy in cardiac fuel and protein homeostasis. J Mol Cell Cardiol 2015; 84:223-226.

More>

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Protective Effects of Combination of Radix Astragali and Radix Salviae Miltiorrhizae on Kidney of Spontaneously Hypertensive Rats and Renal Intrinsic Cells

Chemical Composition, Pharmacological Effects and Clinical Applications of Cinobufacini

Immunomodulatory Function of Pien Tze Huang in T Cell-Mediated Anti-tumor Activity against B16-F10, MC38 and Hep1-6 Tumor Models

Safflower Yellow Inhibits Progression of Hepatocellular Carcinoma by Modulating Immunological Tolerance via FAK Pathway

Immunoregulatory and Anti-cancer Activities of Combination Treatment of Novel Four-Herb Formula and Doxorubicin in 4T1-Breast Cancer Bearing Mice

Related Author

No data

Related Institution

Department of Nephrology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine

Central Laboratory, Affiliated Hospital of Shandong University of Traditional Chinese Medicine

First Clinical Medical College, Shandong University of Traditional Chinese Medicine

Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, Shaanxi University of Chinese Medicine

State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Science, Xiamen University

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰