Ethanol Extract of <italic style="font-style: italic">Glycyrrhiza uralensis</italic> Fisch: Antidiarrheal Activity in Mice and Contraction Effect in Isolated Rabbit Jejunum

WEN Jing; ZHANG Jian-wu; LYU Yuan-xia; ZHANG Hui; DENG Kai-xi; CHEN Hong-xue; WEI Ying

doi:10.1007/s11655-022-3536-5

Your Location：

Home >

Browse articles >

Ethanol Extract of Glycyrrhiza uralensis Fisch: Antidiarrheal Activity in Mice and Contraction Effect in Isolated Rabbit Jejunum

Original Article | Updated：2023-04-05

- Ethanol Extract of Glycyrrhiza uralensis Fisch: Antidiarrheal Activity in Mice and Contraction Effect in Isolated Rabbit Jejunum
- Chinese Journal of Integrative Medicine Vol. 29, Issue 4, Pages: 325-332(2023)
- Affiliations：
  
  Department of Pharmacy, North Sichuan Medical College, Nanchong, Sichuan Province (637100), China
- Author bio：
  
  Dr. WEI Ying, E-mail: 414697669@qq.com
- Funds：
- DOI：10.1007/s11655-022-3536-5
  CLC：
- Published：2023-04，
  
  Published Online：23 August 2022，
  
  Accepted：24 March 2022
Scan for full text
WEN Jing, ZHANG Jian-wu, LYU Yuan-xia, et al. Ethanol Extract of Glycyrrhiza uralensis Fisch: Antidiarrheal Activity in Mice and Contraction Effect in Isolated Rabbit Jejunum. [J]. Chinese Journal of Integrative Medicine 29(4):325-332(2023)
DOI：

WEN Jing, ZHANG Jian-wu, LYU Yuan-xia, et al. Ethanol Extract of Glycyrrhiza uralensis Fisch: Antidiarrheal Activity in Mice and Contraction Effect in Isolated Rabbit Jejunum. [J]. Chinese Journal of Integrative Medicine 29(4):325-332(2023) DOI： 10.1007/s11655-022-3536-5.

摘要

Abstract

Objective:

To evaluate the antidiarrheal effect of ethanol extract of

Glycyrrhiza uralensis

Fisch root (GFR)

in vivo

and jejunal contraction

in vitro

Methods:

In vivo

50 mice were divided into negative control

positive control (verapamil)

low-

medium- and high-dose GFR (250

500

000 mg/kg) groups by a random number table

10 mice in each group. The antidiarrheal activity was evaluated in castor oil-induced diarrhea mice model by evacuation index (EI).

In vitro

the effects of GFR (0.01

0.03

0.1

0.3

and 10 g/L) on the spontaneous contraction of isolated smooth muscle of rabbit jejunum and contraction of pretreated by Acetylcholine (ACh

10 μmol/L) and KCl (60 mmol/L) were observed for 200 s. In addition

CaCl

was accumulated to further study its mechanism after pretreating jejunal smooth muscle with GFR (1 and 3 g/L) or verapamil (0.03 and 0.1 μmol/L) in a Ca

-free-high-K

solution containing ethylene diamine tetraacetic acid (EDTA).

Results:

GFR (500 and 1

000 mg/kg) significantly reduced EI in castor oil-induced diarrhea model mice (

0.01). Meanwhile

GFR (0.01

0.03

0.1

0.3

and 10 g/L) inhibited the spontaneous contraction of rabbit jejunum (

0.05 or

0.01). Contraction of jejunums samples pretreated by ACh and KCl with 50% effective concentration (EC

) values was 1.05 (0.71–1.24)

0.34 (0.29–0.41) and 0.15 (0.11–0.20) g/L

respectively. In addition

GFR moved the concentration-effect curve of CaCl

down to the right

showing a similar effect to verapamil.

Conclusions:

GFR can effectively reduce diarrhea and inhibit intestinal contraction

and these antidiarrheal effects may be based on blocking L-type Ca

channels and muscarinic receptors.

关键词

Keywords

Glycyrrhiza uralensis FischantidiarrhealspasmolysisChinese medicine

references

Ukeermu K, Shatal K, Xue Q, Deng LJ, Xu XY. Effects of Zirike Granule on diarrhea and small intestine propulsion in mice. Chin Pharmacol (Clin) 2012;28:158-161.

Chen HZ, Lin GW, eds. Practical internal medicine. 13th edition. Beijing: People's Medical Publishing House;2009:1948-1949.

Thapar N, Sanderson IR. Diarrhoea in children: an interface between developing and developed countries. Lancet 2004;363:641-653.

Wang WP, ed. Pediatrics. 8th edition. Beijing: People's Medical Publishing House; 2013:1286-1287.

Han YF, Wang YL, Li JX. Research progress on pathogenesis and intervention of diarrhea-predominant irritable bowel syndrome. Liaoning Univ Tradit Chin Med(Chin) 2018;20:114-117.

Kosek M, Bernm C, Guerrant RL. The global burden of diarrhoeal disease, as estimated from studies published between 1992 and 2000. Bull World Health Organ 2003;81:197-204.

Zhang SS, Wang CJ, Li YF, Wang N. Expert consensus on diagnosis and treatment of diarrhea in traditional Chinese medicine. J Tradit Chin Med (Chin) 2017;58:1256-1260.

Chinese Pharmacopoeia Commission. The Chinese Pharmacopoeia. Beijing: China Medical and Technology Press; 2020:88.

Deng TM, Peng C, Peng DY, Yu NJ, Chen WD, Wang L. Research progress on chemical constituents and pharmacological effects of Glycyrrhizae Radix et Rhizoma and discussion of Q-markers. China J Chin Mater Med(Chin) 2021;46:2660-2676.

Zhang Z, Yang L, Hou J. Molecular mechanisms underlying the anticancer activities of licorice flavonoids. J Ethnopharmacol 2021;267:113635.

Zhang Q, Ye M. Chemical analysis of the Chinese herbal medicine Gan-Cao (licorice). J Chromatogr A 2009;1216:1954-1969.

Li J. Clinical observation on 37 cases of functional abdominal pain treated by Shaoyao Gancao Decoction. Chin J Ethnomed Ethnopharm (Chin) 2020;29:62-64.

Zhou ZH, Ji JB, Wang ZC. Effects of Shaoyao Gancao Decoction on visceral hypersensitivity of irritable bowel syndrome rats. Chin J Integr Tradit West Med (Chin)2017;37:575-578.

Wu B, Chang ZP, Deng GF, Shao YY. Intervention effect and mechanism of Shaoyao Gancao Decoction on post-inflammatory irritable bowel syndrome in rats. J Shanxi Med Univer (Chin) 2021;52:605-611.

Zheng JQ. Preliminary study on pharmaceutical mechanism of total flavonoids from Eupatorium Odoratum Linn. against E. Coli diarrhea in mice [dissertation]. Zhanjiang: Guangdong Ocean University; 2016.

Liu DN. Flavonoids in Fructus Aurantii Immaturus: ultrasound-assisted deep eutectic solvent extraction and effects on 5-FU induce diarrhea mice [dissertation]. Chongqing: Southwest University; 2020.

Wu LF, Li YT, Tang YZ. Research progress on chemical constituents and pharmacological activities of Shaoyao Gancao Decoction. Drug Eval Res (Chin) 2021;44:7.

Chen Y, Wu MX, Liu J, Ma XJ, Shi JL, Wang SN, et al. Acute and sub-acute oral toxicity studies of the aqueous extract from radix, radix with cortex and cortex of Psammosilene tunicoides in mice and rats. J Ethnopharmacol 2018;213:199-209.

Li Y, Li J, Liu X. Antidiarrheal activity of methanol extract of Sophora tonkinensis in mice and spasmolytic effect on smooth muscle contraction of isolated jejunum in rabbits. Pharm Biol 2019;57:477-484.

Gong XP, Sun YY, Chen W. Anti-diarrheal and anti-inflammatory activities of aqueous extract of the aerial part of Rubia cordifolia. BMC Complement Altern Med 2017;17:20.

Tadesse WT, Hailu AE, Gurmu AE. Experimental assessment of antidiarrheal and antisecretory activity of 80% methanolic leaf extract of Zehneriascabra in mice. BMC Complement Altern Med 2014;14:460.

Alanı's AD, Calzada F, Cervantes JA. Antibacterial properties of some plants used in Mexican traditional medicine for the treatment of gastrointestinal disorders. J Ethnopharmacol 2005;100:153-157.

Velázquez C, Calzada F, Torres J, González F, Ceballos G. Antisecretory activity of plants used to treat gastrointestinal disorders in Mexico. J Ethnopharmacol 2006;103:66-70.

Palombo EA. Phytochemicals from traditional medicinal plants used in the treatment of diarrhoea: modes of action and effects on intestinal function. Phytother Res 2006;20:717-724.

Piper BJ, Alinea AA, Wroblewski JR, Graham SM, Chung DY, McCutcheon LRM, et al. A quantitative and narrative evaluation of Goodman and Gilman's pharmacological basis of therapeutics. Pharmacy (Basel) 2020;8:1-20.

Wambe H, Noubissi PA, Fokam Tagne MA, Fankem GO, Kamtchouing L, Ngakou MJ, et al. Anti-shigellosis activity of cola anomala water/ethanol pods extract on Shigella flexneri-induced diarrhea in rats. Biomed Res Int 2019;6706230.

Samuel ES, Dalia GM, Patricia CE, Francisco AC, Juan CSS. Spasmolytic effect of Mentha pulegium L. involves ionic flux regulation in rat ileum strips. J Smooth Muscle Res 2010;46:107-177.

Aleem A, Janbaz KH. Dual mechanisms of anti-muscarinic and Ca2+ antagonistic activities to validate the folkloric uses of Cyperus niveus Retz. as antispasmodic and antidiarrheal. J Ethnopharmacol 2018;213:138-148.

Guo H, Zhang J, Gao W. Gastrointestinal effect of methanol extract of Radix Aucklandiae and selected active substances on the transit activity of rat isolated intestinal strips. Pharm Biol 2014;52:1141-1149.

Guo H, Zhang J, Gao W. Anti-diarrhoeal activity of methanol extract of Santalum album L. in mice and gastrointestinal effect on the contraction of isolated jejunal in rats. J Ethnopharmacol 2014;154:704-710.

Kase S. Relationship between the antidiarrhoeal effects of Hange-Shashin-To and its active componentes. Phytother Res 1999;13:468-473.

Mahmood H, Chaudhry MA, Masood Z, Saeed MA, Adnan S. A mechanistic evaluation of the traditional uses of Nepeta ruderalis in gastrointestinal and airway disorders. J Pharm Biol 2017;55:1017-1021.

Polyanna SN, Douglas SC, Valdelânia GS, Izabela BCL, Daniel BN, Francisca BMS, et al. Antidiarrheal activity of α-terpineol in mice. Biomed Pharmacother 2019;110:631-640.

Wei YY, Sun LL, Fu ST. HEF-19-induced relaxation of colonic smooth muscles and the underlying mechanisms. World J Gastroenterol 2013;19:5314-5319.

Salud PG, Maria M, Daniel ZM, Laura G, Miguel ZS. Antispasmodic effects and action mechanism of essential oil of Chrysactinia mexicana A. gray on rabbit Ileum. Molecules 2016;21:783-795.

Zhang J, Shao ZW, Gao Y, Wang QY. Diastolic effect of quercetin on isolated intestinal smooth muscle of rabbits and its mechanism. Chin J App Physiol (Chin) 2013;29:162-165.

Xu J, Zhang L, Hou XH, Li TW, Gao QH, Zhang SY, et al. Effects of Lianpo Drink on gastric motility of functional dyspepsia rats. China J Tradit Chin Med Pharm (Chin) 2022;2:1018-1021.

Tezuka A, Ishihata A, Aita T, Katano Y. Aging-related alterations in the contractile responses to acetylcholine, muscarinic cholinoceptors and cholinesterase activities in jejunum and colon of the male Fischer 344 rats. Exp Gerontol 2004;39:91-100.

Gilani AH, Shah AJ, Yaeesh S. Presence of cholinergic and calcium antagonist constituents in Saussurea lappa explains its use in constipation and spasm. Phytother Res 2007;21:541-544.

Ali MZ, Mehmood MH, Saleem M, Gilani AH. The use of Euphorbia hirta L. (Euphorbiaceae) in diarrhea and constipation involves calcium antagonism and cholinergic mechanisms. BMC Complement Alternat Med 2020;20:14-30.

More>

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Chemical Composition, Pharmacological Effects and Clinical Applications of Cinobufacini

Immunomodulatory Function of Pien Tze Huang in T Cell-Mediated Anti-tumor Activity against B16-F10, MC38 and Hep1-6 Tumor Models

Safflower Yellow Inhibits Progression of Hepatocellular Carcinoma by Modulating Immunological Tolerance via FAK Pathway

TRAIL and Celastrol Combinational Treatment Suppresses Proliferation, Migration, and Invasion of Human Glioblastoma Cells via Targeting Wnt/β-Catenin Signaling Pathway

Immunoregulatory and Anti-cancer Activities of Combination Treatment of Novel Four-Herb Formula and Doxorubicin in 4T1-Breast Cancer Bearing Mice

Related Author

No data

Related Institution

Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, Shaanxi University of Chinese Medicine

State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Science, Xiamen University

Fujian Pien Tze Huang Enterprise Key Laboratory of Natural Medicine Research and Development

Department of General Surgery, First People's Hospital of Hangzhou Lin'an District

Research Center of Neuroscience, School of Basic Medical Sciences, Chongqing Medical University

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Ethanol Extract of Glycyrrhiza uralensis Fisch: Antidiarrheal Activity in Mice and Contraction Effect in Isolated Rabbit Jejunum

DOI：10.1007/s11655-022-3536-5

摘要

Abstract

关键词

Keywords

references