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Acupoint Autohemotherapy Attenuates Atopic Dermatitis Lesions by Regulating Th1/Th2 Balance in DNCB-Induced BALB/c Mice
- Chinese Journal of Integrative Medicine Vol. 28, Issue 7, Pages: 612-619(2022)
- Affiliations：
1.School of Traditional Chinese Medicine, Southern Medical University, Guangzhou (510515), China
2.Department of Traditional Chinese Medicine, Affiliated Dongguan People's Hospital, Southern Medical University, Dongguan, Guangdong Province (523058), China
3.Department of Rheumatology and Immunology, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Shenzhen, Guangdong Province (518020), China
4.School of Basic Medical Sciences,Southern Medical University, Guangzhou (510515), China
5.Guangzhou Center for Disease Control and Prevention, Guangzhou (510515), China
- Author bio：
Prof. ZHANG Dong-shu, E-mail: nyzds@sina.com
- Funds：
DOI：10.1007/s11655-022-3579-7
CLC：Published：01 July 2022，
Accepted：25 March 2022
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Zhi-wen ZENG, Jin-quan HUANG, Yong CHEN, et al. Acupoint Autohemotherapy Attenuates Atopic Dermatitis Lesions by Regulating Th1/Th2 Balance in DNCB-Induced BALB/c Mice. [J]. Chinese Journal of Integrative Medicine 28(7):612-619(2022)
Zhi-wen ZENG, Jin-quan HUANG, Yong CHEN, et al. Acupoint Autohemotherapy Attenuates Atopic Dermatitis Lesions by Regulating Th1/Th2 Balance in DNCB-Induced BALB/c Mice. [J]. Chinese Journal of Integrative Medicine 28(7):612-619(2022) DOI： 10.1007/s11655-022-3579-7.
摘要
目的:
2
评价自血穴位注射疗法 (Acupoint Autohemotherapy
A-AHT) 对1-氯-2
4-二硝基苯 (1-chloro-2
4-dinitrobenzene
DNCB) 诱导的特应性皮炎 (Atopic dermatitis
AD) 小鼠Th1/Th2免疫失衡的调节作用.
方法:
2
将30只BALB/c小鼠随机分为5组
包括正常对照组 (Normal control
NC) 、AD模型组 (AD) 、自血穴位注射组 (A-AHT) 、自血非穴注射组 (Sham acupoint Autohemotherapy
sA-AHT) 、生理盐水穴位注射组 (Acupoint injection of normal saline
A-NS)
每组6只小鼠. 采用DNCB 致敏小鼠建立实验性AD小鼠模型. 实验第8天起
除NC组和AD组外
其余各组小鼠隔日干预一次
共28天. A-AHT组小鼠抽取自体全血 (autologous whole blood
AWB) 注入双侧足三里 (ST 36) 、曲池 (LI 11) 穴
每穴0.05 mL; sA-AHT组小鼠则将AWB注入对应的非穴 (双侧足三里、曲池穴外旁开5 mm) . A-NS组小鼠于双侧足三里、曲池穴注射生理盐水0.05mL. 采用特应性皮炎严重程度评分 (SCORAD)评价小鼠背部皮损严重程度
每周一次; 运用酶联免疫吸附试验 (ELISA) 检测小鼠血清总免疫球蛋白E (Ig-E) 、干扰素γ (IFN-γ) 和白细胞介素4 (IL-4) 表达水平
流式细胞术 (FCM) 分析小鼠脾细胞Th1/Th2比值
免疫组织化学法 (IHC) 评估皮损组织T-bet和GATA-3的表达.
结果:
2
与AD组相比
A-AHT和sA-AHT两治疗组小鼠SCORAD评分与血清IgE水平下降 (
P
<
0.05或
P
<
0.01) ; A-AHT、sA-AHT和A-NS三组小鼠血清IL-4表达下降
IFN-γ/IL-4比值上升 (
P
<
0.05或
P
<
0.01) ; 而只有A-AHT治疗能够调节AD小鼠Th1/Th2失衡
增加其转录因子T-bet表达并上调T-bet/GATA3比值 (
P
<
0.05) .
结论:
2
自血穴位注射可以通过调节Th1/Th2免疫失衡改善AD小鼠症状.
Abstract
Objective:
2
To evaluate the therapeutic effects of acupoint autohemotherapy (A-AHT) on 1-chloro- 2
4-dinitrobenzene (DNCB)-induced atopic dermatitis (AD) in mice focusing on regulating T helper 1/T helper 2 (Th1/Th2) immune responses.
Methods:
2
Thirty BALB/c mice were divided into 5 groups by a random number table
including normal control (NC)
AD model (AD)
A-AHT
sham A-AHT (sA-AHT)
and acupoint injection of normal saline (A-NS) groups
6 mice per group. Mice were challenged by DNCB for the establishment of experimental AD model. On the 8th day
except for the NC and AD groups
the mice in the other groups received management once every other day for a total of 28 days. For the A-AHT and sA-AHT groups
0.05 mL of autologous whole blood (AWB) was injected into bilateral Zusanli (ST 36) and Quchi (LI 11) and sham-acupoints (5 mm lateral to ST 36 and LI 11)
respectively. The A-NS group was administrated with 0.05 mL of normal saline by acupoint injection into ST 36 and LI 11. Dermatitis severity for dorsal skin of mice was determined using the Severity Scoring of Atopic Dermatitis (SCORAD) every week. The total immunoglobulin E (IgE)
interleukin-4 (IL-4)
and interferon-gamma (IFN-γ) cytokine levels in serum were examined by enzyme-linked immunosorbent assay (ELISA). Spleen Th1/Th2 expression were analyzed via flow cytometry and immunohistochemical assay was used to detect T-box expressed in T cell (T-bet) and GATA-binding protein 3 (GATA3) expressions in skin lesions of mice.
Results:
2
Compared with the AD group
both A-AHT and sA-AHT reduced the SCORAD index and serum IgE level (
P
<
0.05 or
P
<
0.01); A-AHT
sA-AHT and A-NS down-regulated serum IL-4 level and upregulated IFN-γ/IL-4 ratio (
P
<
0.05 or
P
<
0.01); A-AHT regulated the Th1/Th2 shift specifically and increased the related transcription factors such as T-bet expression and T-bet/GATA3 ratio (
P
<
0.05).
Conclusion:
2
A-AHT showed significant effectiveness on the AD model mice
through regulating Th1/Th2 immune responses.
关键词
Keywords
acupoint autohemotherapyatopic dermatitisTh1/Th2immunoglobulin E
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