FOLLOWUS
Baicalin Ameliorates Corticosterone-Induced Depression by Promoting Neurodevelopment of Hippocampal via mTOR/GSK3β Pathway
- Chinese Journal of Integrative Medicine Vol. 29, Issue 5, Pages: 405-412(2023)
- Affiliations：
1.School of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang (050200), China
2.Hebei Province Academy of Chinese Medicine Sciences, Shijiazhuang (050031), China
- Author bio：
Dr. PEI Lin, E-mail: peilin13831190309@126.com
- Funds：
DOI：10.1007/s11655-022-3590-z
CLC：Published：2023-05，
Published Online：06 January 2023，
Accepted：01 July 2022
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WANG Zhe, CHENG Ya-ting, LU Ye, et al. Baicalin Ameliorates Corticosterone-Induced Depression by Promoting Neurodevelopment of Hippocampal via mTOR/GSK3β Pathway. [J]. Chinese Journal of Integrative Medicine 29(5):405-412(2023)
WANG Zhe, CHENG Ya-ting, LU Ye, et al. Baicalin Ameliorates Corticosterone-Induced Depression by Promoting Neurodevelopment of Hippocampal via mTOR/GSK3β Pathway. [J]. Chinese Journal of Integrative Medicine 29(5):405-412(2023) DOI： 10.1007/s11655-022-3590-z.
摘要
Abstract
Objective:
2
To investigate the role of hippocampal neurodevelopment in the antidepressant effect of baicalin.
Methods:
2
Forty male Institute of Cancer Research mice were divided into control
corticosterone (CORT
40 mg/kg)
CORT+baicalin-L (25 mg/kg)
CORT+baicalin-H (50 mg/kg)
and CORT+fluoxetine (10 mg/kg) groups according to a random number table. An animal model of depression was established by chronic CORT exposure. Behavioral tests were used to assess the reliability of depression model and the antidepressant effect of baicalin. In addition
Nissl staining and immunofluorescence were used to evaluate the effect of baicalin on hippocampal neurodevelopment in mice. The protein and mRNA expression levels of neurodevelopment-related factors were detected by Western blot analysis and real-time polymerase chain reaction
respectively.
Results:
2
Baicalin significantly ameliorated the depressive-like behavior of mice resulting from CORT exposure and promoted the development of dentate gyrus in hippocampus
thereby reversing the depressive-like pathological changes in hippocampal neurons caused by CORT neurotoxicity. Moreover
baicalin significantly decreased the protein and mRNA expression levels of glycogen synthase kinase 3β (GSK3β)
and upregulated the expression levels of cell cycle protein D1
p-mammalian target of rapamycin (mTOR)
doublecortin
and brain-derived neurotrophic factor (all
P
<
0.01). There were no significant differences between baicalin and fluoxetine groups (
P
>
0.05).
Conclusion:
2
Baicalin can promote the development of hippocampal neurons via mTOR/GSK3β signaling pathway
thus protect mice against CORT-induced neurotoxicity and play an antidepressant role.
关键词
Keywords
baicalindepressionneurodevelopmenthippocampal neuronalmammalian target of rapamycin/glycogen synthase kinase 3β pathway
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