Dihydromyricetin Alleviates H9C2 Cell Apoptosis and Autophagy by Regulating CircHIPK3 Expression and PI3K/AKT/mTOR Pathway

ZHANG Zhi-ying; LIU Chao; WANG Peng-xiang; HAN Yi-wei; ZHANG Yi-wen; HAO Mei-li; SONG Zi-xu; ZHANG Xiao-ying

doi:10.1007/s11655-022-3687-4

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Dihydromyricetin Alleviates H9C2 Cell Apoptosis and Autophagy by Regulating CircHIPK3 Expression and PI3K/AKT/mTOR Pathway

Original Article | Updated：2023-04-27

- Dihydromyricetin Alleviates H9C2 Cell Apoptosis and Autophagy by Regulating CircHIPK3 Expression and PI3K/AKT/mTOR Pathway
- Chinese Journal of Integrative Medicine Vol. 29, Issue 5, Pages: 434-440(2023)
- Affiliations：
  
  1.Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, Shaanxi Province (712082), China
  2.Key Laboratory of High Altitude Environment and Gene Related to Disease of Tibet Ministry of Education, School of Medicine, Xizang Minzu University, Xianyang, Shaanxi Province(712082), China
  3.School of Finance Economics, Xizang Minzu University, Xianyang, Shaanxi Province (712082), China
  4.School of Information Engineering, Xizang Minzu University, Xianyang, Shaanxi Province (712082), China
- Author bio：
  
  Dr. ZHANG Xiao-ying, E-mail:zzy20210726@163.com
- Funds：
- DOI：10.1007/s11655-022-3687-4
  CLC：
- Published：2023-05，
  
  Published Online：07 December 2022，
  
  Accepted：29 June 2022
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ZHANG Zhi-ying, LIU Chao, WANG Peng-xiang, et al. Dihydromyricetin Alleviates H9C2 Cell Apoptosis and Autophagy by Regulating CircHIPK3 Expression and PI3K/AKT/mTOR Pathway. [J]. Chinese Journal of Integrative Medicine 29(5):434-440(2023)
DOI：

ZHANG Zhi-ying, LIU Chao, WANG Peng-xiang, et al. Dihydromyricetin Alleviates H9C2 Cell Apoptosis and Autophagy by Regulating CircHIPK3 Expression and PI3K/AKT/mTOR Pathway. [J]. Chinese Journal of Integrative Medicine 29(5):434-440(2023) DOI： 10.1007/s11655-022-3687-4.

摘要

Abstract

Objective:

To investigate the effect and potential mechanism of dihydromyricetin (Dmy) on H9C2 cell proliferation

apoptosis

and autophagy.

Methods:

H9C2 cells were randomly divided into 7 groups

namely control

model

EV (empty pCDH-CMV-MCS-EF1-CopGFP-T2A-Puro vector)

IV (circHIPK3 interference)

Dmy (50 μmol/L)

Dmy+IV

and Dmy+EV groups. Cell proliferation and apoptosis were detected by cell counting kit-8 assay and flow cytometry

respectivley. Western blot was used to evaluate the levels of light chain 3 Ⅱ/Ⅰ (LC3Ⅱ/Ⅰ)

phospho-phosphoinositide 3-kinase (p-PI3K)

protein kinase B (p-AKT)

and phospho-mammalian target of rapamycin (p-mTOR). The level of circHIPK3 was determined using reverse transcriptase polymerase chain reaction. Electron microscopy was used to observe autophagosomes in H9C2 cells.

Results:

Compared to H9C2 cells

the expression of circHIPK in H9C2 hypoxia model cells increased significantly (

0.05). Compared to the control group

the cell apoptosis and autophagosomes increased

cell proliferation rate decreased significantly

and the expression of LC3Ⅱ/Ⅰ significantly increased (all

0.05). Compared to the model group

the rate of apoptosis and autophagosomes in IV

Dmy

and Dmy+IV group decreased

the cell proliferation rate increased

and the expression of LC3Ⅱ/Ⅰ decreased significantly (all

0.05). Compared to the control group

the expressions of p-PI3K

p-AKT

and p-mTOR in the model group significantly reduced (

0.05)

whereas after treatment with Dmy and sh-circHIPK3

the above situation was reversed (

0.05).

Conclusion:

Dmy plays a protective role in H9C2 cells by inhibiting circHIPK expression and cell apoptosis and autophagy

and the mechanism may be related to PI3K/AKT/mTOR pathway.

关键词

Keywords

dihydromyricetincircHIPKautophagycell apoptosisPI3K/AKT/mTOR

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