Effects of Total Saponins from <italic style="font-style: italic">Dioscorea Nipponica</italic> Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An <italic style="font-style: italic">in vitro</italic> Study

ZHOU Qi; SUN Hui-juan; LIU Shu-min

doi:10.1007/s11655-022-3721-6

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Effects of Total Saponins from Dioscorea Nipponica Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An in vitro Study

Original Article | Updated：2023-01-05

- Effects of Total Saponins from Dioscorea Nipponica Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An in vitro Study
- Chinese Journal of Integrative Medicine Vol. 29, Issue 1, Pages: 44-51(2023)
- Affiliations：
  
  1.Research Institute of Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin (150040), China
  2.Graduate School, Heilongjiang University of Chinese Medicine, Harbin (150040), China
  3.Technological Innovation Team of Basic Theory Study Research of Institution of Higher Education in Heilongjiang Province, Heilongjiang University of Chinese Medicine, Harbin (150040), China
- Author bio：
  
  Prof. LIU Shu-min, E-mail: keji-liu@163.com
- Funds：
- DOI：10.1007/s11655-022-3721-6
  CLC：
- Published：01 January 2023，
  
  Published Online：12 July 2022，
  
  Accepted：30 October 2021
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ZHOU Qi, SUN Hui-juan, LIU Shu-min. Effects of Total Saponins from Dioscorea Nipponica Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An in vitro Study. [J]. Chinese Journal of Integrative Medicine 29(1):44-51(2023)
DOI：

ZHOU Qi, SUN Hui-juan, LIU Shu-min. Effects of Total Saponins from Dioscorea Nipponica Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An in vitro Study. [J]. Chinese Journal of Integrative Medicine 29(1):44-51(2023) DOI： 10.1007/s11655-022-3721-6.

摘要

Abstract

Objective:

To investigate and reveal the underlying mechanism of the effect of total saponins from

Dioscoreae nipponica

Makino (TSDN) on the arachidonic acid pathway in monosodium urate (MSU) crystal-induced M1-polarized macrophages.

Methods:

M1 polarization of RAW264.7 cells were induced by 1 μg/mL lipopolysaccharide (LPS). The methylthiazolyldiphenyl-tetrazolium bromide method was then used to screen the concentration of TSDN. MSU (500 μg/mL) was used to induce the gouty arthritis model. Afterwards

10 μg/L TSDN and 8 μmol/L celecoxib

which was used as a positive control

were added to the above LPS and MSU-induced cells for 24 h. The mRNA and protein expressions of cyclooxygenase (COX) 2

5-lipoxygenase (5-LOX)

microsomal prostaglandin E synthase derived eicosanoids (mPGES)-1

leukotriene B (LTB)4

cytochrome P450 (CYP) 4A

and prostaglandin E

(PGE

) were tested by real-time polymerase chain reaction and Western blotting

respectively. The enzyme-linked immunosorbent assay was used to test the contents of M1 markers

including inducible nitric oxid synthase (NOS) 2

CD80

and CD86.

Results:

TSDN inhibited the proliferation of M1 macrophages and decreased both the mRNA and protein expressions of COX2

5-LOX

CYP4A

LTB4

and PGE

(

0.01) while increased the mRNA and protein expression of mPGES-1 (

0.05 or

0.01). TSDN could also significantly decrease the contents of NOS2

CD80

and CD86 (

0.01).

Conclusion:

TSDN has an anti-inflammation effect on gouty arthritis in an

in vitro

model by regulating arachidonic acid signaling pathway.

关键词

Keywords

gouty arthritisDioscorea nipponica Makinoarachidonic acidsignaling pathwayM1/M2 polarization

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Related Author

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Related Institution

Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, National Urological Cancer Center

Department of Gastroenterology, Xiyuan Hospital, China Academy of Chinese Medical Sciences

Graduate School of China Academy of Chinese Medical Sciences

Department of Internal Medicine, Jining Medical College Affiliated Hospital

Department of Gastroenterology, Henan University of Chinese Medicine Affiliated Hospital

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Effects of Total Saponins from Dioscorea Nipponica Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An in vitro Study

DOI：10.1007/s11655-022-3721-6

摘要

Abstract

关键词

Keywords

references