Objective:

2

To reveal the anti-inflammatory mechanism of Guanxin Ⅴ

which is prescribed for ventricular remodeling in clinical practice.

Methods:

2

Guanxin Ⅴ-

ventricular remodeling-

and inflammation-related targets were obtained through an integrated strategy of virtual screening and systematic pharmacology

and then the shared targets were visualised with a Venn diagram. Guanxin Ⅴ network and the protein-protein interaction network were drawn

and enrichment analysis was conducted. Finally

the main results obtained from the integrated strategy were validated by molecular docking and

in vivo

experiments.

Results:

2

A total of 251

11

425

and 15

246 Guanxin Ⅴ-

ventricular remodeling-

and inflammation-related targets were acquired

respectively. Then

211 shared targets were considered to contribute to the mechanism of ventricular remodeling treated by Guanxin Ⅴ. Guanxin network and the protein-protein interaction network were drawn

and enrichment analysis showed some cardiovascular-related biological processes and signaling pathways. Molecular docking revealed that the Guanxin Ⅴ-derived compounds could align with key targets. Final

in vivo

experiments proved that Guanxin Ⅴ reverses ventricular remodeling by inhibiting inflammation.

Conclusion:

2

Guanxin Ⅴ relieves ventricular remodeling by regulating inflammation

which provides new ideas for the anti-ventricular remodeling mechanism of Guanxin Ⅴ.