Objective:

2

To explore the potential molecular mechanism of tetrahydropalmatine (THP) on acute myocardial ischemia (AMI).

Methods:

2

First

the target genes of THP and AMI were collected from SymMap Database

Traditional Chinese Medicine Database and Analysis Platform

and Swiss Target Prediction

respectively. Then

the overlapping target genes between THP and AMI were evaluated for Grene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and protein-protein interaction network analysis. The binding affinity between the protein and THP was assessed by molecular docking. Finally

the protective effects of THP on AMI model and oxygen and glucose deprivation (OGD) model of H9C2 cardiomyocyte were explored and the expression levels of target genes were detected by RT-qPCR

in vivo

and

in vitro

.

Results:

2

MMP9

PPARG

PTGS2

SLC6A4

ESR1

JAK2

GSK3B

NOS2 and AR were recognized as hub genes. The KEGG enrichment analysis results revealed that the potential target genes of THP were involved in the regulation of PPAR and hormone pathways. THP improved the cardiac function

as well as alleviated myocardial cell damage. Furthermore

THP significantly decreased the RNA expression levels of MMP9

PTGS2

SLC6A4

GSK3B and ESR1 (

P

<

0.05

P

<

0.01) after AMI.

In vitro

THP significantly increased H9C2 cardiomyocyte viability (

P

<

0.05

P

<

0.01) and inhibited the RNA expression levels of PPARG

ESR1 and AR (

P

<

0.05

P

<

0.01) in OGD model.

Conclusions:

2

THP could improve cardiac function and alleviate myocardial injury in AMI. The underlying mechanism may be inhibition of inflammation

the improvement of energy metabolism and the regulation of hormones.