Objective:

2

To study the effect of Shexiang Tongxin Dropping Pill (STDP) on angiogenesis in diabetic cardiomyopathy mice with coronary microcirculation dysfunction (CMD).

Methods:

2

According to a random number table

6 of 36 SPF male C57BL/6 mice were randomly selected as the control group

and the remaining 30 mice were injected with streptozotocin intraperitoneally to replicate the type 1 diabetes model. Mice successfully copied the diabetes model were randomly divided into the model group

STDP low-dose group [15 mg/(kg•d)]

medium-dose group [30 mg/(kg•d)]

high-dose group [60 mg/(kg•d)]

and nicorandil group [15 mg/(kg•d)]

6 in each group. The drug was given by continuous gavage for 12 weeks. The cardiac function of mice in each group was detected at the end of the experiment

and coronary flow reserve (CFR) was detected by chest Doppler technique. Pathological changes of myocardium were observed by hematoxylin-eosin staining

collagen fiber deposition was detected by masson staining

the number of myocardial capillaries was detected by platelet endothelial cell adhesion molecule-1 staining

and the degree of myocardial hypertrophy was detected by wheat germ agglutinin staining. The expression of the vascular endothlial growth factor (VEGF)/endothelial nitric oxide synthase (eNOS) signaling pathway-related proteins in myocardial tissue was detected by Western blot.

Results:

2

Compared with the model group

medium- and high-dose STDP significantly increased the left ventricular ejection fraction and left ventricular fraction shortening (

P

<

0.01)

obviously repaired the disordered cardiac muscle structure

reduced myocardial fibrosis

reduced myocardial cell area

increased cap

illary density

and increased CFR level (all

P

<

0.01). Western blot showed that high-dose STDP could significantly increase the expression of VEGF and promote the phosphorylation of vascular endothelial growth factor receptor 2

phosphoinositide 3-kinase

protein kinase B

and eNOS (

P

<

0.05 or

P

<

0.01).

Conclusion:

2

STDP has a definite therapeutic effect on diabetic CMD

and its mechanism may be related to promoting angiogenesis through the VEGF/eNOS signaling pathway.