Objective:

2

To explore the potential of metanephric mesenchymal cells (MMCs) for osteogenesis and naringin's ability to enhance this process and its molecular mechanism.

Methods:

2

Porcine MMCs at 70 days of gestation were used as tool cells

cultured in osteogenic induction medium

identified by immunocytochemistry staining. Osteogenic potential of porcine MMCs and naringin's ability to enhance this process was tested by detecting changes in cell viability

alkaline phosphatase (ALP) activity

the expression of runt-related transcription factor 2 (Runx2)

osteopontin (OPN) and osteocalcin (OCN)

and the formation of mineralized nodules

and the application of the p38 signaling pathway inhibitor SB203580 vitiated the osteogenesis-promoting effect of naringin.

Results:

2

Immunocytochemical staining showed that the cells were Vimentin and Six2(+)

E-cadherin and CK-18(–). Naringin can activate the p38 signaling pathway to enhance the osteogenesis of porcine MMCs by increasing cell viability

ALP activity

the expressions of Runx2

OPN and OCN

and t

he formation of mineralized nodules (

P

<

0.05). The application of p38 signaling pathway inhibitor SB203580 vitiated the osteogenesis-promoting effect of naringin

manifested by decreased ALP activity

the expressions of Runx2

OPN and OCN

and the formation of mineralized nodules (

P

<

0.05).

Conclusion:

2

Naringin

the active ingredient of Chinese herbal medicine

Rhizoma Drynariae

for nourishing Shen (Kidney) and strengthening bone

enhances the osteogenic differentiation of renal MMCs through the p38 signaling pathway.