Objective:

2

To investigate the effect of chrysophanol

a phytochemical derived from

Radix et Rhizoma Rhei

on HepG2 liver cancer cells.

Methods:

2

HepG2 cell line was treated with different concentrations chrysophanol (0–100 μmol/L) for 24 h. The cell counting kit 8 assay was employed to assess cell viability. Intracellular calcium levels were examined using Fluo-4 AM and Mag-fluo-4 AM staining

followed by flow cytometry analysis. Mitochondrial membrane potential was measured with JC-1 assay kit. Additionally

the expressions of key proteins such as p-JNK

Bax

cytochrome c (Cyt C)

cleaved caspase-3 (cCaspase-3)

and caspase-8 were analyzed by Western blot. The inhibitory effects of chrysophanol on the invasion of cells were determined using a Transwell assay. Analysis of invasiveness was conducted by wound healing assay.

Results:

2

Chrysophanol significantly reduced the proliferation of HepG2 liver cancer cells by affecting intracellular calcium distribution

diminishing mitochondrial membrane potential

and enhancing the expressions of p-JNK

Bax

Cyt C

cCaspase-3

and caspase-8 in the groups treated with 75 or 100 μmol/L chrysophanol compared to the control group (

P

<

0.05). Additionally

75 and 100 μmol/L chrysophanol exhibited inhibitory effects on cell migration and wound healing.

Conclusion:

2

Chrysophanol demonstrates potential against HepG2 liver cancer cells

suggesting its potential use as a therapeutic agent for liver cancer treatment.