Objective:

2

To investigate the inhibitory effect of

Celastrus orbiculatus

extracts (COE) on the proliferation of lymphoma cells and the immune regulation ability on inflammation and thrombophilia

in vivo

.

Methods:

2

The 38B9 lymphoma cells were treated with COE (160μg/mL) and CTX (25 μmol/L). The apoptosis rate and cell cycle of each group were detected by flow cytometry. The secretion of inflammatory factors

including interleukin (IL)-6

IL-10

and tumor necrosis factor α (TNF-α)

in cell supernatant was detected by enzyme-linked immunosorbent assay (ELISA).

In vivo

BALB/c mice were subcutaneously injected with 38B9 lymphoma cells to establish lymphoma model. COE (3 mg•kg

-1

•d

-1

) and CTX (40 mg•kg

-1

•d

-1

) were administered to the model mice

respectively. The expression of plasma inflammatory factors (IL-6

IL-10 and TNF-α) and thrombus indexes

including D-dimer (D-D)

von Willebrand factor (vWF) and tissue factor (TF)

were detected by ELISA before tumor bearing (1 d)

after tumor formation (14 d) and after intervention (21 d). PicoGreen dsDNA was used to detect the level of serum neutrophil extracellular traps (NETs). Flow cytometry was used to detect the expression of platelet activation marker calcium-dependent lectin-like receptor 2 (CLEC-2). The tumor growth and survival of mice were recorded.

Results:

2

The 38B9 lymphoma cells were apoptotic after the intervention of COE and CTX. The ratio of G

2

-M phase cells decreased in COE intervented cells compared with the control cells (

P

<

0.05)

and S phase cells

decreased in CTX intervented cells (

P

<

0.05). Also

the secretion level of IL-6 was significantly reduced after COE or CTX intervention (

P

<

0.05)

and IL-10 was significantly increased (

P

<

0.05). Furthermore

the tumor mass was reduced

and the median survival time was longer in COE and CTX intervented tumor-bearing mice than in non-intervented mice. The significantly lower levels of TNF-α

IL-6

NETs

TF

DD and CLEC-2

as well as higher IL-10 were observed in COE and CTX treatment mice in comparision with the control mice (

P

<

0.05).

Conclusion:

2

COE has a mild and stable anti-tumor effect

which can reduce the secretion of inflammatory factors by lymphoma cells and regulate thrombophilic state caused by tumor inflammatory microenvironment.