Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP<sup>+</sup>-Induced PC12 Cells

LUAN Zhen-xian; TANG Xiang-lin; HAO Fei-ran; LI Min; LI Shao-dan; YANG Ming-hui

doi:10.1007/s11655-025-3707-2

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Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP⁺-Induced PC12 Cells

Original Article | Updated：2025-08-25

- Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP⁺-Induced PC12 Cells
- Chinese Journal of Integrative Medicine Vol. 31, Issue 9, Pages: 830-836(2025)
- Affiliations：
  
  Medical Department of Traditional Chinese Medicine, the Sixth Medical Center of the PLA General Hospital, Beijing (100048), China
- Author bio：
  
  Prof. YANG Ming-hui, E-mail: Ymh9651@sina.com
- Funds：
- DOI：10.1007/s11655-025-3707-2
  CLC：
- Accepted：05 May 2024，
  
  Published Online：25 March 2025，
  
  Published：2025-09
- Accepted：
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LUAN Zhen-xian, TANG Xiang-lin, HAO Fei-ran, et al. Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP⁺-Induced PC12 Cells[J]. Chinese journal of integrative medicine, 2025, 31(9): 830-836.
DOI：

LUAN Zhen-xian, TANG Xiang-lin, HAO Fei-ran, et al. Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP⁺-Induced PC12 Cells[J]. Chinese journal of integrative medicine, 2025, 31(9): 830-836. DOI： 10.1007/s11655-025-3707-2.

摘要

Abstract

Objective:

To investigate the effects of Bushen Huoxue Granule on the ubiquitin-proteasome system (UPS) in an

in vitro

model of Parkinson's disease.

Methods:

After treated with 1-methyl-4-phenylpyridinium (MPP

1 mmol/L) for 24 h

the cells were incubated with drug-free serum

Madopar-containing serum or Bushen Huoxue Granule-containing serum (BCS

10%

and 20%) for another 24 h. The levels of α-synuclein (α-syn)

tyrosine hydroxylase (TH) and UPS-related proteins were detected by Western blot. The expression levels of α-syn in PC12 cells were also analyzed by Western blot after treated with proteasome inhibitor MG132 and WT-α-syn plasmid transfection

respectively

as well as the alterations induced by subsequent BCS intervention. Immunocytochemistry was performed to determine the changes in α-syn phosphorylation at serine 129 (pSer129-α-syn) expression. The 20S proteasome levels were measured by enzyme-linked immunosorbnent assay.

Results:

BCS (volume fraction 20%) intervention could alleviate the MMP

-induced cell viability decrease (

0.05). In the MPP

treated cells

α-syn was up-regulated

while TH and proteins of UPS such as ubiquitin (Ub)

Ub binding with Ub-activating enzyme (UBE1)

Parkin and Ub C-terminal hydrolase-1 (UCHL-1) were down-regulated (

0.05). BCS intervention could attenuate the above changes (

0.05). The activity of BCS on blocking α-syn accumulation was weakened by MG132 (

0.05). While α-syn level was significantly increased in cells transfected with plasmid

and reduced by BCS intervention (

0.05). pSer129-α-syn was increased in MPP

-induced PC12 cells

whereas decreased by later BCS intervention (

0.05). The 20S proteasome activity of MPP

-induced PC12 cells was decreased

but increased after BCS intervention (

0.05).

Conclusion:

BCS intervention protected UPS function

increased 20S proteasome activity

promoted pathological α-syn clearance

restored cell viability

and reversed the damage caused by MPP

in the

in vitro

model of Parkinson's disease.

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Related Author

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Related Institution

Department of Neurology, Shanghai Public Health Clinical Center, Fudan University

Department of Neurology, Zhongshan Hospital South Branch, Fudan University

Institute of Neurology, Institutes of Integrative Medicine, Fudan University

Geriatrics Department, the Third Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine

College of Integrative Medicine, Fujian University of Traditional Chinese Medicine

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Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP+-Induced PC12 Cells

DOI：10.1007/s11655-025-3707-2

摘要

Abstract

关键词

Keywords

references

Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP⁺-Induced PC12 Cells