Objective:

2

To explore the anti-photoaging properties of salvianolic acid B (Sal B).

Methods:

2

The optimal photoaging model of human immortalized keratinocytes (HaCaT cells) were constructed by expose to ultraviolet B (UVB) radiation. The cells were divided into control

model and different concentrations of Sal B groups. Cell viability was measured via cell counting kit-8. Subsequently

the levels of oxidative stress

including reactive oxygen species (ROS)

hydroxyproline (Hyp)

catalase (CAT)

and glutathione peroxidase (GSH-Px) were detected using the relevant kits. Silent information regulator 1 (SIRT1) protein level was detected using Western blot. The binding pattern of Sal B and SIRT1 was determined via molecular docking.

Results:

2

Sal B significantly increased the viability of UVB-irradiated HaCaT cells (

P

<

0.05 or

P

<

0.01). Sal

B effectively scavenged the accumulation of ROS induced by UVB (

P

<

0.05 or

P

<

0.01). In addition

Sal B modulated oxidative stress by increasing the intracellular concentrations of Hyp and CAT and the activity of GSH-Px (

P

<

0.05 or

P

<

0.01). The Western blot results revealed a substantial increase in SIRT1 protein levels following Sal B administration (

P

<

0.05). Moreover

Sal B exhibited good binding affinity toward SIRT1

with a docking energy of –7.5 kCal/mol.

Conclusion:

2

Sal B could improve the repair of photodamaged cells by alleviating cellular oxidative stress and regulating the expression of SIRT1 protein.