Anti-tumor Effects of <italic style="font-style: italic">Pollen Pini</italic> on Hepatocarcinoma SMMC-7721 Cells via Modulation of PI3K-Akt Signaling Pathway

CHEN Yuan-hong; XU Wei-xin; YU Wen-jun; WANG Chun-fang; GUO Chun; LUO Yan-hong

doi:10.1007/s11655-025-4132-2

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Anti-tumor Effects of Pollen Pini on Hepatocarcinoma SMMC-7721 Cells via Modulation of PI3K-Akt Signaling Pathway

Original Article | Updated：2026-08-04

- Anti-tumor Effects of Pollen Pini on Hepatocarcinoma SMMC-7721 Cells via Modulation of PI3K-Akt Signaling Pathway
- Chinese Journal of Integrative Medicine Vol. 32, Issue 2, Pages: 120-127(2026)
- Affiliations：
  
  1.Department of Pathogenic Biology and Immunology, Youjiang Medical University for Nationalities, Baise, Guangxi Zhuang Autonomous Region (533000), China
  2.Key Laboratory of Basic Research and Transformation of Tumor Immunity and Infectious Diseases, Youjiang Medical University for Nationalities, Baise, Guangxi Zhuang Autonomous Region (533000), China
  3.Department of Pharmacy, Yancheng Clinical College of Xuzhou Medical University/the First People's Hospital of Yancheng, Yancheng, Jiangsu Province (224001), China
  4.Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC (27707), USA
  5.Center for Clinical Laboratory Diagnosis and Research, the Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi Zhuang Autonomous Region (533000), China
  6.Department of Anatomy, School of Basic Medicine Science, Youjiang Medical University for Nationalities, Baise, Guangxi Zhuang Autonomous Region (533000), China
  7.Modern Industrial College of Biomedicine and Great Health, Youjiang Medical University for Nationalities, Baise, Guangxi Zhuang Autonomous Region (533000), China
- Author bio：
  
  Prof. LUO Yan-hong, E-mail: 00505@ymun.edu.cn
- Funds：
- DOI：10.1007/s11655-025-4132-2
  CLC：
- Accepted：01 January 2025，
  
  Online First：15 August 2025，
  
  Published：2026-02
- Accepted：
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CHEN Yuan-hong, XU Wei-xin, YU Wen-jun, et al. Anti-tumor Effects of Pollen Pini on Hepatocarcinoma SMMC-7721 Cells via Modulation of PI3K-Akt Signaling Pathway[J]. Chinese Journal of Integrative Medicine, 2026, 32(2): 120-127. DOI: 10.1007/s11655-025-4132-2.
DOI：

CHEN Yuan-hong, XU Wei-xin, YU Wen-jun, et al. Anti-tumor Effects of Pollen Pini on Hepatocarcinoma SMMC-7721 Cells via Modulation of PI3K-Akt Signaling Pathway[J]. Chinese Journal of Integrative Medicine, 2026, 32(2): 120-127. DOI: 10.1007/s11655-025-4132-2. DOI：

摘要

Abstract

Objectives:

To explore the effect of

Pollen Pini

(PP) on SMMC-7721 hepatoma cells.

Methods:

The anti-proliferative effects of PP on SMMC-7721 cells were evaluated using cell counting kit-8 assay following 48 h treatment with concentrations ranging from 1.25 to 37.50 μg/μL. Flow cytometry analysis at the half maximal inhibitory concentration (IC

) revealed significant apoptosis induction and cell cycle alterations. For

in vivo

evaluation

SMMC-7721 xenograft-bearing nude mice were administered either vehicle (0.9% NaCl) or PP(500 mg/kg) via intraperitoneal injection every other day for 3 weeks. Subsequent tumor analysis included mass spectrometry-based proteomics and examination of phosphoinositide-3 kinase (PI3K)/protein kinase B (Akt)/Myc pathway proteins by immunohistochemistry and Western blot. Combination therapy with 25 μmol/L PI3K inhibitor and PP (IC

) showed optimal efficacy

with Western blot revealing maximal protein modulation at 24 h.

Results:

PP had an anti-tumor effect on SMMC-7721 cells

in vitro

with the IC

concentration of 18.94 mg/mL. PP could promote the death of SMMC-7721 cells (

0.01) and regulate the cell cycle more in G

phase cells (

0.01). After the treatment effect of PP

the protein content of SMMC-7721 cells changed and the contents of cancer-promoting proteins PI3K

Akt and Myc decreased (

0.01). PI3K inhibitor could reduce the proliferation of SMMC-7721 cells

and PI3K inhibitor combined with PP significantly re

duced the expression of PI3K in SMMC-7721 cells(

0.01).

Conclusion:

PP has an anti-tumor effect on SMMC-7721 cells through the PI3K-Akt signaling pathway.

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Anti-tumor Effects of Pollen Pini on Hepatocarcinoma SMMC-7721 Cells via Modulation of PI3K-Akt Signaling Pathway

DOI：10.1007/s11655-025-4132-2

摘要

Abstract

关键词

Keywords

references